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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (12): 2139-2149.doi: 10.3724/SP.J.1006.2009.02139

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS •     Next Articles

  QTL Mapping of Protein Related Traits in Soybean[Glycine max (L.) Merr.]

LIU Shun-Hu1,4,ZHOU Rui-Bao2,*, YU De-Yue1,CHEN Shou-Yi3,GAI Jun-Yi1,*   

  1. 1Soybean Research Institute of Nanjing Agricultural University,National Center for Soybean Improvement,and National Key Laboratory for Crop Genetics and Germplasm Enhancement,Nanjing 210095,China;2Soybean Processing Research Institute,Henan University of Technology,zhengzhou 450012,China,3Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101;4Shandong Jining College,Qufu 273155,China
  • Received:2009-05-07 Revised:2009-09-24 Online:2009-12-10 Published:2009-10-13
  • Contact: GAI Jun-Yi，E-mail: sri@njau.edu.cn; Tel: 025-84395405;ZHOU Rui-Bao，E-mail: rbzhou0615@163.com

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Abstract:

Soybean processing industry places emphasis on the quality of soybean protein which is related to the protein components, mainly 11S, 7S and 11S/7S, and their subunit constituents. In the improvement of soybean protein quality, the knowledge of genetic structure of the traits related to protein quality is of great importance. Therefore, the present paper was aimed at mapping QTLs of 16 traits, including protein content, protein plus fat content, fat content, 11S, 7S, 11S/7S, and subunit groups. Two populations, RIKY population with 184 recombinant inbred lines derived from Kefeng 1×Nannong 1138-2 and BIEX population with 114 BC1F2 lines derived from (Essex×ZDD2315) ×ZDD2315, were used to map QTLs with the softwares of composite interval mapping (CIM), multiple interval mapping (MIM) of WinQTL Cartographer Ver. 2.5 and the inclusive composite interval mapping (ICIM) of IciMapping. The results showed that there were totally 17+ QTLs detected with 11 for protein content, fat content and total content of protein and fat, and 1+ and 3+ for 11S subunit groups and 7S subunit groups, respectively, in RIKY, as well as totally 21+ detected with only 2+ for protein content, fat content and protein plus fat content, but 9+ and 6+ for 11S subunit groups and 7S subunit groups, respectively, in BIEX. There was no shared QTL detected for all 16 traits in both populations, indicating that the 16 traits between RIKY and BIEX have completely different genetic systems, and there existed obvious genetic differences between two parents of RIKY in protein content, fat content and protein plus fat content but less genetic differences in 11S subunit groups and 7S subunit groups, and those of BIEX were on the contrary. The group of protein content, fat content and protein plus fat content and the group of 11S, 7S and 11S/7S had common QTLs, showing their common genetic base, but there were no common QTLs in the groups of 11S subunit and 7S subunit. The results from QTL mapping and segregation analysis showed jointly that both major genes and minor genes contributed a large part of phenotypic variations for all 16 traits, suggesting that both major genes and minor genes should be considered in the breeding for protein-related traits.

Key words: Soybean, Protein content, Protein plus fat content, 11S, 7S, 11S/7S, Subunit group, QTL mapping

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