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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (1): 36-46.doi: 10.3724/SP.J.1006.2010.00036

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Density-Enhanced Genetic Linkage Map of RIL Population NJRIKY and Its Impacts on Mapping Genes and QTLs and QTLs in Soybean

ZHOU Bin1,XING Han1,CHEN Shou-Yi2,GAI Jun-Yi1,*   

  1. 1Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvemet/State Key Laboratory of Crop Genetics and Germplasm Enhancement， Nanjing 210095，China；2 State Key Laboratory of Plant Gemomics，Institute of Genetics and developmental Biology,Chinese Academy of Sciences,Being 100101,China
  • Received:2009-05-08 Revised:2009-10-02 Online:2010-01-12 Published:2009-11-17
  • Contact: Gai Jun-Yi, E-mail: sri@njau.edu.cn; Tel: 025-84395405

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Abstract:

A high-density genetic linkage map with informative markers is essential for plant genome analysis, including gene mapping, identification of quantitative trait locus (QTL), map-based cloning, and physical map construction. Though four genetic linkage maps for the recombinant inbred line population NJRIKY derived from (Kefeng 1×Nannong 1138-2) have been established already, there are still problems of precision and accuracy in mapping genes and QTLs due to insufficiency of genetic information and number of markers. A total of 401 polymorphic SSR markers were screened out from 967 ones for density-enhancement of the previous maps. Along with other marker data, a new genetic linkage map was constructed by using Mapmaker/Exp 3.0b, with 553 markers, including 316 SSR, 197 RFLP, 39 EST and one morphologic markers, spanning 25 linkage groups, covering total length 2 071.6 cM of the soybean genome, with an average marker interval distance of 3.70 cM. In comparison with the old map, the number of gaps larger than 20 cM decreased from forty-two to two on the enhanced map. Using this map to relocate the seven SMV resistant genes, Rsc-3, Rsc-7, Rsc-9, Rsc-13, Rsa, Rn1 and Rn3 were mapped on LG D1b again with distances to the flanking markers all less than 6 cM, among them, Rsc-9, Rn1 and Rsa less than 1 cM and Rsc-13 co-segregating with EST-SSR marker GMKF168a. After re-mapping the QTLs for eight agronomic traits, 42 QTLs were detected on 12 linkage groups, with 20 of them accounted for more than 10% of the total variation, respectively, and their marker intervals obviously shortened.

Key words: Soybean, Genetic linkage map, SSR markers, QTL

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