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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (01): 74-78.doi: 10.3724/SP.J.1006.2011.00074

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Transferability Analysis of Cassava EST-SSR and Genomic-SSR Markers in Jatropha and Rubber Tree

WEN Ming-Fu,CHEN Xin,WANG Hai-Yan,LU Cheng,WANG Wen-Quan*   

  1. Institute of Tropical Bioscience & Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
  • Received:2010-05-04 Revised:2010-08-01 Online:2011-01-12 Published:2010-10-09
  • Contact: WANG Wen-Quan,E-mail:wquanw@hainan.net,Tel:0898-66894533

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Abstract: Euphorbiaceae family includes abundant economic species, such as rubber tree, cassava, castor bean and Jatropha. Cassava (Manihot esculenta Crantz) ranks in the sixth food crop in the world. In China, cassava is also an important tropical economic crop. The genomic-SSRs derived from cassava genome, and EST-SSRs derived from expressed sequence tags (ESTs). In this study, the transferability of 419 pairs of EST-SSR primer and 182 pairs of genomic-SSR primer from cassava was tested in five Jatropha lines and two rubber tree lines. The result showed that the transferability rate of cassava EST-SSR in Jatropha and rubber tree was 55.85% and 38.90%, and the transferability rate of cassava genomic-SSR in Jatropha and rubber tree was 37.36% and 26.37%, respectively. The transferability EST-SSR was higher for cssava than for genomic-SSR. Meanwhile, the transferability of cassava EST-SSR and genomic-SSR was higher in Jatropha than in rubber tree. These results suggested that the cassava SSR can be used for comparative mapping, gene tagging and QTL mapping among cassava, Jatropha and rubber tree.

Key words: Jatropha, Rubber tree, Transferability, EST-SSR, Genomic-SSR

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