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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (07): 1221-1231.doi: 10.3724/SP.J.1006.2012.01221

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity of Arachis Accessions Detected by EST-SSR from Cultivated Peanut (Arachis hypogaea L.)

TANG Mei1,2,CHEN Yu-Ning2,REN Xiao-Ping2,HUANG Li2,ZHOU Xiao-Jing2,YAN Hai-Yan1,JIANG Hui-Hang2,*   

  1. 1 College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China; 2 Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China
  • Received:2012-01-13 Revised:2012-04-16 Online:2012-07-12 Published:2012-05-11
  • Contact: 姜慧芳, E-mail: peanutlab@oilcrops.cn E-mail:tangmeitangyong@yahoo.com.cn

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Abstract: The amplification efficiency and application potential for the research on peanut genetic diversity by using cultivars-derived EST-SSR primers in 89 Arachis aeecssions were assessed. Based on randomly selected 235 EST-SSR primer pairs, we detected 223 amplification bands in the 89 accessions with the amplification efficiency of 94.89%. Among them 53 primer pairs exhibited 206 polymorphic bands, which showed 1–12 bands per primer pair with an average of 3.89. The polymorphism index ranged from 0.044 to 4.040 with an average of 1.173. The average similarity coefficient was 0.685 among 89 peanut accessions, ranging from 0.442 to 0.976. The accessions from the same section were mainly clustered together, peanut cultivars were grouped into the section of Arachis, and A. duranensis had a close relation to cultivated peanut, which is coincident with botanical classification. According to the statistical analysis from the results of oil content and SSR data in wild peanuts, some interesting bands such as POCR437-180/170 were found to be potential for candidate markers related to oil content. BLAST analysis indicated that the alignment sequence of primer POCR437 corresponded to the coding genes of malonyl-CoA-ACP transacylase and acyl-carrier-protein, both of which are involved in fatty acid synthesis.

Key words: Wild peanut, EST-SSR, Effectiveness, Cluster analysis

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