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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (5): 861-866.doi: 10.3724/SP.J.1006.2009.00861

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Increasing Density of Wheat Genetic Linkage Map with Molecular Makers

LI Yan-Qiu1，SU Zhi-Fang12**，WANG Li-Xin1，JI Wei1，YAO Ji3，ZHAO Chang-Ping1*   

  1. 1Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing 100097,China;2Farming-grazing Science Department of Hetao University,Linhe 015000,China;3College of Life Science and technology,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2008-10-15 Revised:2008-12-31 Online:2009-05-12 Published:2009-03-23
  • Contact: ZHAO Chang-Ping E-mail:bjhwc2003@yahoo.com.cn

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Abstract:

High density genetic linkage map is the groundwork for mapping gene or quantitative trait loci, map-based cloning and marker-assisted selection. To increase the marker density on genetic linkage map of wheat (Triticum aestivum L.), the double haploid (DH) population derived from Jinghua 1/Xiaobai Dongmai and the recombinant inbred lines (RILs) of Nongda 015/Fuzhuang 30 were used in this study. A total of 339 polymorphic markers between the DH lines and 343 polymorphic markers between the RIL lines were detected. Using the DH population, 208 markers were mapped on 21 chromosomes, covering 3 493.6 cM; and using the RIL population, 299 markers were mapped on 34 linkage groups with the average distance of 15.5 cM. The two linkage maps had 56 consistent markers in the similar regions of chromosomes. Using Joinmap 4.0 software, ten linkage groups from the two linkage maps were integrated. This linkage map was composed of 217 markers and covered 956.2 cM of wheat genome with an average distance of 4.4 cM between markers. The proportion of segregation distortion loci was 3.2–55.6% on eight chroosomes. Most markers in this map had the consistent locations with those mentioned in previous report, however, five SSR markers were located on different chromosomes. The results enhance the density of wheat linkage map and provide more information for users.

Key words: Wheat, SSR, EST-SSR, SCAR, Linkage map


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