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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (03): 469-476.doi: 10.3724/SP.J.1006.2011.00469

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Epistatic and Additive Effects of QTLs for Grain Shape Using Single Segment Substitution Lines in Rice (Oryza sativa L.)

ZHAO Fang-Ming1,ZHANG Gui-Quan2,ZENG Rui-Zhen2,YANG Zheng-Lin1,LING Ying-Hua1,SANG Xian-Chun1,HE Guang-Hua1,*   

  1. 1 Rice Research Institute, Southwest University / Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Chongqing 400716, China; 2 Guangdong Key Laboratory of Plant Molecular Breeding / South China Agricultural University, Guangzhou 510642,China
  • Received:2010-06-19 Revised:2010-12-01 Online:2011-03-12 Published:2011-01-17
  • Contact: 何光华, E-mail: hegh@swu.edu.cn

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Abstract: It is important to accelerate breeding process by pyramiding genes purposefully with molecular marker-assisted selection methods. Additive and epistatic effects of genes are the key to the success in genes pyramiding breeding. In this paper, additive and epistatic effects of QTLs for rice grain shape were analyzed using 16 single segment substitution lines (SSSL) and 15 double segment substitution lines (DSSL). A total of nine QTLs were identified on the Chromosomes 2, 3, 4, and 10 respectively, containing four for grain length, one for grain width and four for ratio of grain length to width. Furthermore, seven pairs of digenic interactions were detected for the grain shape. Among them, three interactions occurred between two loci with obvious effects on the traits, one interaction did not have obvious effects at both two loci, and three interactions occurred between one locus with and another without main effect at the single-locus level. Again, the results also indicated that the epistatic effects were different when the same QTL controlling grain length was pyramided with various SSSLs. Only when the epistatic effects were syntropic with the additive effects of the target genes, the pyramiding effect could be obvious. Moreover, longer or shorter grain was difficult to be obtained by pyramiding two QTLs controlling long grain or two QTLs for short grain. The results are important for improving grain shape by molecular marker assisted selection.

Key words: Rice, Single segment substitution lines, Grain shape QTL, Additive effects, Epistatic effects

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