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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (04): 717-722.doi: 10.3724/SP.J.1006.2011.00717

• RESEARCH NOTES • Previous Articles     Next Articles

Genetic Analysis and Mapping of glup-t Gene for 57H Mutant in Rice

TIAN Meng-Xiang1,2,CHEN Tao1,ZHANG YA-Dong1,ZHU Zhen1,ZHAO Qing-Yong1,ZHOU Li-Hui1,YAO Shu1,WANG Yan-Ping1,WANG Cai-Lin1,*   

  1. 1 Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice R&D Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China; 2 College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2010-08-16 Revised:2010-11-28 Online:2011-04-12 Published:2011-02-24
  • Contact: 王才林, E-mail: clwang@jaas.ac.cn, Tel: 025-84390307

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Abstract: Glutelins are major storage protein and account for 80% of the total protein found in starchy endosperm in rice. They are synthesized on rough endoplasmic reticulum as 57 kD precursors, and then processed into acidic and basic subunits in PB-II. Mutation of the gene controlling the synthesis of glutelin will result in an formation with high amount of 57 kD glutelin precursor, named 57H mutant. In this study, we reported a novel rice 57H spontaneous mutant, Xilaoshuya, which showed increased amount of 57 kD and 13 kD polypeptides, the decreased amounts of 37–39 kD and 22–23 kD polypeptides. Four F2 segregation populations derived from crosses between Xilaoshuya and Wuyunjing 7, 02428 were used to study the inheritance and gene mapping for 57H mutant. The results suggested that, the mutant trait in Xilaoshuya was controlled by a pair of recessive nuclear gene and designated tentatively as glup-t. By means of molecular marker technique, the glup-t gene was mapped between CAPS4-3 and Indel4-7, Indel4-8 on the long arm of chromosome 4, and all of the genetic distances to the three markers were 0.26 cM.

Key words: Rice, Glutelin, 57H mutant, glup-t, Gene mapping

[1]Liu Q-Q(刘巧泉), Zhou L-H(周丽慧), Wang H-M(王红梅), Gu M-H(顾铭洪). Advances on biosynthesis of rice seed storage proteins in molecular biology. Mol Plant Breed (分子植物育种), 2008, 6(1): 1–15 (in Chinese with English abstract)
[2]Yamagata H, Sugimoto T, Tanaka K, Kasai Z. Biosynthesis of storage proteins in developing rice seeds. Plant Physiol, 1982, 170: 1094–1100
[3]Sarker S C, Ogawa M, Takahashi M, Asada K. Processing of a 57-kD precursor peptide to subunits of rice glutelin. Plant Cell Physiol, 1986, 27: 1579–1586
[4]Kumamaru T, Uemura Y, Inoue Y, Takemoto Y, Siddiqui S U, Ogawa M, Hara-Nishimura I, Satoh H. Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed. Plant Cell Physiol, 2010, 51: 38–46
[5]Kumamaru T, Satoh H, Iwata N, Omura T, Ogawa M, Tanaka K. Mutants for rice storage proteins: 1. Screening of mutants for rice storage proteins of protein bodies in the starchy endosperm. Theor Appl Genet, 1988, 76: 11–16
[6]Zheng T Q, Shen W B, Zhu S S, Zhai H Q, Wan J M. Status and perspectives on the researches of rice glutelin mutants. Agric Sci China, 2003, 2(3): 237–244
[7]Tian H-D(田怀东), Zhang B-X(张变香). Diversity of phenotypes of endosperm storage proteins of rice 57H mutants and their classification. Bull Bot Res (植物研究) 2010, 30(3): 283–288 (in English with Chinese abstract)
[8]Kumamaru T, Satoh H, Iwata N, Omura T, Ogawa M. Mutants for rice storage proteins: III. Genetic analysis of mutants for storage proteins of protein bodies in the starchy endosperm. Jpn J Genet, 1987, 62: 333–339
[9]Satoh H, Kumamaru T, Yoshimura S, Ogawa M. New 57 kDa glutelin genes on chromosome 9 in rice. Rice Genet Newslett, 1994, 11: 158–161
[10]Kumamaru T, Uemura Y, Takemoto Y, Ogawa M, Satoh H. High-resolution mapping of glup3 gene accumulating high amount of glutelin precursor. Rice Genet Newslett, 2002, 19: 62–63
[11]Wang Y H, Zhu S S, Liu S J, Jiang L, Chen L M, Ren Y L, Han X H, Liu F, Ji S L, Liu X, Wan J M. The vacuolar processing enzyme OsVPE1 is required for efficient glutelin processing in rice. Plant J, 2009, 58: 606–617
[12]Sato M, Takemoto Y, Kumamaru T, Satoh H. Mapping of glup4 gene for highly accumulating glutelin precursor polypeptides in rice. Rice Genet Newslett, 2003, 20: 42–43
[13]Ueda Y, Satoh H, Satoh M, Takemoto Y, Kumamaru T, Ogawa M. Inheritance mode of Glup5 gene and the genetic relationships with other 57 mutant genes. Rice Genetics Newsletters, 2004, 21: 49–50
[14]Satoh M, Ishihara D, Tian H D, Takemoto Y, Kumamaru, T, Satoh H. Gene mapping and immunocytochemistry of rice glup6 mutant. Rice Genetics Newsletters, 2003, 20: 43–44
[15]Ueda Y, Sugino A, Takemoto Y, Satoh M, Kumamaru T, Ogawa M, Satoh H. A novel 57H mutant gene, glup7, located on chromosome 4. Rice Genet Newslett, 2004, 21: 51–54
[16]Takemoto Y, Coughlan S J, Okita T W, Satoh H, Ogawa M, Kumamaru T. The rice mutant esp-2 greatly accumulates the glutelin Precursor and deletes the protein disulfide isomerase. Plant Physiol, 2002, 4: 1212–1222
[17]Wang Y-P(王艳平), Tian M-X(田孟祥), Tang L-H(汤陵华), Fang X-W(方先文), Wang C-L(王才林). The glutelin content analysis of rice 57H glutelin mutants. Jiangsu Agric Sci (江苏农业科学)，2009, (6): 34–36 (in Chinese)
[18]Jiang S-M(江绍玫), Zhu S-S(朱速松), Shen W-B(沈文飚), Zheng T-Q(郑天清), Wang Y-H(王益华), Jiang L(江玲), Wang C-M(王春明), Xu L-L(徐朗莱), Wan J-M(万建民). Research on methods for screening rice mutant s of storage proteins. J Nanjing Agric Univ (南京农业大学学报), 2003, 26(4): 17–20 (in Chinese with English abstract)
[19]Tian M-X(田孟祥), Chen T(陈涛), Zhang Y-D(张亚东), Zhu Z(朱镇), Zhao L(赵凌), Zhao Q-Y(赵庆勇), Zhou L-H(周丽慧), Wang Y-P(王艳平), Wang C-L(王才林). Design and validation of two indel markers for low glutelin content gene (lgc1) in rice. Mol Plant Breed (分子植物育种), 2010, 8(2): 340–344 (in Chinese with English abstract)
[20]Dellaport S L, Wood J, Hicks J B. A plant DNA minipreparation: Plant Mol Biol Rep, 1983, 1: 19–21
[21]Zhao Q-Y(赵庆勇), Zhu Z(朱镇), Zhang Y-D(张亚东), Zhao L(赵凌), Chen T(陈涛), Zhang Q-F(张巧凤), Wang C-L(王才林). Analysis on correlation between heterosis and genetic distance based on simple sequence repeat markers in japonica rice. Chin J Rice Sci (中国水稻科学), 2009, 23(2): 141–147 (in Chinese with English abstract)
[22]Temnykh S, Declerck G, Lukashova A, Lipovich L, Cartinhour S, Mccouch S. Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): frequency, length variation, transposon association, and genetic marker potential. Genome Res, 2001, 11: 1441–1452
[23]Satoh H, Kumamaru T, Ogawa M, Shiraishi M, Im B G, Son M Y, Takemoto Y. Spontaneous 57 kDa mutant in rice. Rice Genet Newslett, 1995, 12: 194–196
[24]Satoh H, Li W X, Takemoto Y, Ito T, Kumamaru T, Qu L Q, Ogawa M. glup4 controlling a 57H character was located on chromosome 12 in rice. Rice Genet Newslett, 1999, 16: 98–100
[25]Tian H D, Kumamaru T, Takemoto Y, Ogawa M, Satoh H. Gene analysis of new 57H mutant gene, glup6, in rice. Rice Genet Newsl, 2001, 18: 48–50
[26]Ahmed S U, Bar-Peled M, Raikhel N V. Cloning and subcellular location of an Arabidopsis receptor-like protein that shares common features with protein-sorting receptors of eukaryotic cells. Plant Physiol, 1997, 114: 325–336
[27]Shimada T, Fuji K, Tamura K, Kondo M, Nishimura M, Hara-Nishimura I. Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana. Proc Natl Acad Sci USA, 2003, 100: 16095–16100
[28]Kim H, Kang H, Jang M, Chang J H, Miao Y S, Jiang L W, Hwang I. Homomeric interaction of AtVSR1 is essential for its function as a vacuolar sorting receptor. Plant Physiol, 2010, 154: 134–148
[29]Shimada T, Koumoto Y, Li L X, Yamazaki M, Kondo M, Nishimura M, Hara-Nishimura I. AtVPS29, a putative component of a retromer complex, is required for the efficient sorting of seed storage proteins. Plant Cell Physiol, 2006, 47: 1187–1194
[30]Li L X, Shimada T, Takahashi H, Ueda Y, Fukao Y, Kondo M, Nishimura M, Hara-Nishimura I. MAIGO2 is involved in exit of seed storage proteins from the endoplasmic reticulum in Arabidopsis thaliana. Plant Cell, 2006, 18: 3535–3547
[31]Tamura K, Takahashi H, Kunieda T, Fuji K, Shimada T, Hara-Nishimura I. Arabidopsis KAM2/GRV2 is required for proper endosome formation and functions in vacuolar sorting and determination of the embryo growth axis. Plant Cell, 2007, 19: 320–332
[32]Shimada T, Yamada K, Kataoka, M, Nakaune S, Koumoto Y, Kuroyanagi M, Tabata S, Kato T, Shinozaki K, Seki M, Kobayashi M, Kondo M, Nishimura M, Hara-Nishimura I. Vacuolar processing enzymes are essential for proper processing of seed storage proteins in Arabidopsis thaliana. J Biol Chem, 2003, 278: 32292–32299
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