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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (04): 723-728.doi: 10.3724/SP.J.1006.2011.00723

• RESEARCH NOTES • Previous Articles     Next Articles

Cloning and Analysis of β-Glucan Synthase Gene AsCSLH in Avena sativa L.

U Bin,ZHANG Zong-Wen*   

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2010-09-10 Revised:2011-01-06 Online:2011-04-12 Published:2011-02-24
  • Contact: 张宗文, E-mail:zongwenz@163.com, Tel: 010-82105685

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Abstract: Beta-glucan [(1→3, 1→4)-β-D-glucan] is one of the important components in oat (Avena sativa L.) that has the function of health protection for human beings. Cloning the β-glucan synthase gene is of significance to reveal the molecular mechanism of β-glucan accumulation and guide oat breeding for β-glucan enhancement. A β-glucan synthase gene, designated AsCSLH, and its genomic DNA sequence were cloned from the oat landrace Xiayoumai using rapid amplification of cDNA ends (RACE) and GenomeWalk from the cDNA, respectively. The AsCSLH gene contains a 2 277 bp open reading frame and encodes a peptide of 758 amino acid residues. Six introns were found in AsCSLH gene with typical GT/AG characteristic after comparison of its sequences of genomic DNA and cDNA. Sequences analysis showed that AsCSLH gene shared the highest identity with rice CSLH gene (BK000084). Structure analysis of predicted encoded protein also confirmed that this cDNA sequence was the oat β-glucan synthase gene. The tissue-specific expression analysis by semiquantitative RT-PCR assays suggested that AsCSLH was expressed in different tissues of oat with the highest level in immature seeds. These results suggested that AsCSLH plays an important role in oat β-glucan synthesis.

Key words: Oat (Avena sativa L.), β-glucan synthase, Gene cloning, Expression analysis

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