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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (04): 717-722.doi: 10.3724/SP.J.1006.2011.00717

• RESEARCH NOTES • Previous Articles     Next Articles

Genetic Analysis and Mapping of glup-t Gene for 57H Mutant in Rice

TIAN Meng-Xiang1,2,CHEN Tao1,ZHANG YA-Dong1,ZHU Zhen1,ZHAO Qing-Yong1,ZHOU Li-Hui1,YAO Shu1,WANG Yan-Ping1,WANG Cai-Lin1,*   

  1. 1 Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice R&D Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China; 2 College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2010-08-16 Revised:2010-11-28 Online:2011-04-12 Published:2011-02-24
  • Contact: 王才林, E-mail: clwang@jaas.ac.cn, Tel: 025-84390307

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Abstract: Glutelins are major storage protein and account for 80% of the total protein found in starchy endosperm in rice. They are synthesized on rough endoplasmic reticulum as 57 kD precursors, and then processed into acidic and basic subunits in PB-II. Mutation of the gene controlling the synthesis of glutelin will result in an formation with high amount of 57 kD glutelin precursor, named 57H mutant. In this study, we reported a novel rice 57H spontaneous mutant, Xilaoshuya, which showed increased amount of 57 kD and 13 kD polypeptides, the decreased amounts of 37–39 kD and 22–23 kD polypeptides. Four F2 segregation populations derived from crosses between Xilaoshuya and Wuyunjing 7, 02428 were used to study the inheritance and gene mapping for 57H mutant. The results suggested that, the mutant trait in Xilaoshuya was controlled by a pair of recessive nuclear gene and designated tentatively as glup-t. By means of molecular marker technique, the glup-t gene was mapped between CAPS4-3 and Indel4-7, Indel4-8 on the long arm of chromosome 4, and all of the genetic distances to the three markers were 0.26 cM.

Key words: Rice, Glutelin, 57H mutant, glup-t, Gene mapping

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