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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 264-274.doi: 10.3724/SP.J.1006.2012.00264

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of QTL for Rice Starch RVA Profile Properties under Different Ecological Sites

YANG Ya-Chun, NI Da-Hu, SONG Feng-Shun, LI Li, LU Xu-Zhong, LI Ze-Fu,YANG Jian-Bo*   

  1. Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
  • Received:2011-05-25 Revised:2011-09-12 Online:2012-02-12 Published:2011-11-07
  • Contact: 杨剑波, E-mail: yjianbo@263.net, Tel: 0551-2160212

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Abstract: Two rice varieties with similar apparent amylose content, Nipponbare (japonica) and 93-11 (indica), were used as parents to establish a recombinant inbred lines population consisting of 190 lines by single seed descent method. The genetic linkage map was constructed with 202 SSR, CAPs and STS markers. Quantitative trait loci (QTLs) were identified for eight rice starch RVA profile properties including peak paste viscosity(PKV), hot paste viscosity(HPV), cool paste viscosity(CPV), breakdown viscosity(BDV), setback viscosity(SBV), consistence viscosity(CSV), peaktime (PeT), pasting temperature (PaT) by composite interval mapping method in three different ecological sites (Lingshui, Hefei, and Huaiyuan). A total of 57 QTLs were identified, with 1 to 14 for each trait, indicating that rice starch RVA profile properties were controlled by multiple genes. Thirteen stable QTLs were detected at two or three sites, among which qCPV-3, qCPV-10b, qSBV-10b, qCSV-3b,and qCSV-10b (explaining, 26.9%, 29.5%, 29.7%, 25.2%, and 28.3% of variance) were detected in all the three sites. Sixteen QTLs were found to have pleiotropy with a single QTL controlling two to six traits (RVA profile properties), the interval RM25032–RM1375 on chromosome 10 controlling six traits (PKV, HPV, CPV, SBV, PaT and CSV).

Key words: Rice, RVA profile properties, Quantitative trait locus, QTL stability, QTL pleiotropy

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