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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 264-274.doi: 10.3724/SP.J.1006.2012.00264

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of QTL for Rice Starch RVA Profile Properties under Different Ecological Sites

YANG Ya-Chun, NI Da-Hu, SONG Feng-Shun, LI Li, LU Xu-Zhong, LI Ze-Fu,YANG Jian-Bo*   

  1. Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
  • Received:2011-05-25 Revised:2011-09-12 Online:2012-02-12 Published:2011-11-07
  • Contact: 杨剑波, E-mail: yjianbo@263.net, Tel: 0551-2160212

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Abstract: Two rice varieties with similar apparent amylose content, Nipponbare (japonica) and 93-11 (indica), were used as parents to establish a recombinant inbred lines population consisting of 190 lines by single seed descent method. The genetic linkage map was constructed with 202 SSR, CAPs and STS markers. Quantitative trait loci (QTLs) were identified for eight rice starch RVA profile properties including peak paste viscosity(PKV), hot paste viscosity(HPV), cool paste viscosity(CPV), breakdown viscosity(BDV), setback viscosity(SBV), consistence viscosity(CSV), peaktime (PeT), pasting temperature (PaT) by composite interval mapping method in three different ecological sites (Lingshui, Hefei, and Huaiyuan). A total of 57 QTLs were identified, with 1 to 14 for each trait, indicating that rice starch RVA profile properties were controlled by multiple genes. Thirteen stable QTLs were detected at two or three sites, among which qCPV-3, qCPV-10b, qSBV-10b, qCSV-3b,and qCSV-10b (explaining, 26.9%, 29.5%, 29.7%, 25.2%, and 28.3% of variance) were detected in all the three sites. Sixteen QTLs were found to have pleiotropy with a single QTL controlling two to six traits (RVA profile properties), the interval RM25032–RM1375 on chromosome 10 controlling six traits (PKV, HPV, CPV, SBV, PaT and CSV).

Key words: Rice, RVA profile properties, Quantitative trait locus, QTL stability, QTL pleiotropy

[1]Shu Q-Y(舒庆尧), Wu D-X(吴殿星), Xia Y-W(夏英武), Gao M-W(高明尉), Anna M C. Relationship between RVA profile character and eating quality in Oryza sativa L. Sci Agric Sin (中国农业科学), 1998, 31(3): 25–29 (in Chinese with English abstract)

[2]Wu D-X(吴殿星), Shu Q-Y(舒庆尧), Xia Y-W(夏英武). Rapid identification of starch viscosity property of early indica rice varieties with different apparent amylose content by RVA profile. Chin J Rice Sci (中国水稻科学), 2001, 15(1): 57–59 (in Chinese with English abstract)

[3]Jia L(贾良), Ding X-Y(丁雪云), Wang P-R(王平荣), Deng X-J(邓晓建). Rice RVA profile characteristics and correlation with the physical/chemical quality. Acta Agron Sin (作物学报), 2008, 34(5): 790–794 (in Chinese with English abstract)

[4]Cai Y-X(蔡一霞), Wang W(王维), Zhu Z-W(朱智伟), Zhang Z-J (张祖建), Yang J-C(杨建昌), Zhu Q-S(朱庆森). The physiochemical characteristics of amylopectin and their relationships to pasting properties of rice flour in different varieties. Sci Agric Sin (中国农业科学), 2006, 39(6): 1122–1129 (in Chinese with English abstract)

[5]Bao J S, Harold C, He P, Zhu L H. Analysis of quantitative trait loci for starch properties of rice based on an RIL population. Acta Bot Sin, 2003, 45(8): 986–994

[6]Bao J S, Wu Y R, Hu B, Wu P, Cui H R, Shu Q Y. QTL for rice grain quality based on a DH population derived from parents with similar apparent amylose content. Euphytica, 2002, 128: 317–324

[7]Bao J S, Xia Y W. Genetic control of paste viscosity characteristics in indica rice. Theor Appl Genet, 1999, 98: 1120–1124

[8]Bao J S, Zheng X W, Xia Y W, He P, Shu Q Y, Lu X, Chen Y, Zhu L H. QTL mapping for the paste viscosity characteristics in rice (Oryza sativa L.). Theor Appl Genet, 2000, 100: 280–284

[9]Zhang Q-F(张巧凤), Zhang Y-D(张亚东), Zhu Z(朱镇), Zhao L(赵凌), Zhao Q-Y(赵庆勇), Xu L(许凌), Wang C-L(王才林). Analysis of inheritance and QTLs of rice starch viscosity (RVA Profile) characteristics. Chin J Rice Sci (中国水稻科学), 2007, 21(6): 592–598 (in Chinese with English abstract)

[10]Gravois K A. Genetic and genotype ×environment effects for rough rice and head rice yield. Crop Sci, 1991, 31: 907–911

[11]Gravois K A, Webb B D. Inheritance of long grain rice amylograph viscosity characteristics. Euphytica, 1997, 97: 25–29

[12]Rogers S O, Bendch A J. Extraction of DNA from plant tissues. Plant Mol Biol Man, 1988, 6: 1–10

[13]McCouch S R, Cho Y G, Yano M. Report on QTL nomenclature. Rice Genet Newsl, 1997, 14: 11–13

[14]Lee M H, Hettiarachchy N S, McNew R W. Physicochemical properties of calcium fortified rice. Cereal Chem, 1995, 72: 352–355

[15]Jane J, Chen Y Y, Lee L F. Effects of amylopectin branch length and amylose content on the gelatinization and pasting properties of starch. Cereal Chem, 1999, 76(5): 629–637

[16]Han X Z, Hamaber B R. Amylopectin fine structure and rice starch paste breakdown. J Cereal Sci, 2001, 34: 279–284

[17]Jiang H W, Dian W M, Wu P. Effect of high temperature on fine structure of amylopectin in rice endosperm by reducing the activity of the starch branching enzyme. Phytochemistry, 2003, 63: 53–59

[18]Cai Y-X(蔡一霞), Wang W(王维), Zhang Z-J(张祖建), Xia G-H(夏广宏), Zhang H-X(张洪熙), Yang J-C(杨建昌), Zhu Q-S(朱庆森). Comparative studies on cooking quality and RVA Profile of several rice varieties under water-and-Dry cultivation. Acta Agron Sin (作物学报), 2003, 29(4): 508–513 (in Chinese with English abstract)

[19]Wei M-G(魏蒙关). QTL Analysis for Stover Yield and Quality Traits and Their Genetic Relationship Using Two Connected F2:3 Populations in Maize. PhD Dissertation of Henan Agriculture University, 2009 (in Chinese with English abstract)

[20]Jansen R C, Van Ooijen J W, Stam P. Genotype-by-environment interaction in genetic mapping of multiple quantitative trait loci. Theor Appl Genet, 1995, 91: 33–37

[21]Wan X Y, Wan J M, Weng J F, Jiang L, Bi J C, Wang C M, Zhai H Q. Stability of QTLs for rice grain dimension and endosperm chalkiness characteristics across eight environments. Theor Appl Genet, 2005, 110(7): 1334–1346

[22]Ryoo N, Yu C, Park C S, Baik M Y, Park I M, Cho M H, Bhoo S H, An G H, Hahn T R, Seong J. Knockout of a starch synthase gene OsSSIIIa/Flo5 causes white-core floury endosperm in rice (Oryza sativa L.). Plant Cell Rep, 2007,26(7):1083–1095

[23]Wu H-K(吴洪恺), Liang G-H(梁国华), Gu Y-J(顾燕娟), Shan L-L(单丽丽), Wang F(王芳),Han Y-P(韩月膨), Gu M-H(顾铭洪). The effect of the Starch-Synthesizing genes on RVA Profile characteristics in rice (Oryza sativa L.). Acta Agron Sin (作物学报), 2006, 32(11): 1597–1603(in Chinese with English abstract)

[24]Jiang H W, Dian W M, Liu F Y, Wu P. Molecular cloning and expression analysis of three genes encoding Starch Synthase II in rice. Planta, 2004, 218: 1062–1070

[25]Zhang Y-S(张永生), Jang L(江玲), Liu X(刘喜), Liu S-J(刘世家), Chen L-M(陈亮明), Zhai H-C(翟虎渠), Wan J-M(万建民). Analysis of QTLs for starch RVA Profile Properties in the superior rice cultivar Koshihikari. Chin J Rice Sci (中国水稻科学), 2010, 24(2): 137–144 (in Chinese with English abstract)

[26]Xiao J, Li J, Yuan L, Tanksley S D. Identification of QTLs affecting traits of agronomic importance in a recombinant inbred population derived from a subspecific rice cross. Theor Appl Genet, 1996, 92: 230–244

[27]Hittalmani S, Huang N, Courtois B, Venuprasad R, Shashidhar H, Zhuang J Y, Zheng K L, Lui G F, Wang G C, Sidhu J S. Identification of QTL for growth and grain yield-related traits in rice acrossnine locations of Asia. Theor Appl Genet, 2003, 107: 679–690

[28]Bao J-S(包劲松), He P(何平), Xia Y-W(夏英武), Chen Y(陈英), Zhu L-H(朱立煌). RVA Profile of rice starch was controlled by Wx gene. Chin Sci Bull, 1999, 44(18): 1973–1976 (in Chinese)

[29]Shen S-Q(沈圣泉), Zhuang J-Y(庄杰云), Shu Q-Y(舒庆尧), Bao J-S(包劲松), Wu D-X(吴殿星), Xia Y-W(夏英武). Analysis of QTLs with main, epistasis and G×E interaction effects of Starch Paste Viscosity in rice. Acta Agron Sin (作物学报), 2005, 31(10): 1289–1294 (in Chinese with English abstract)

[30]Weng J-F(翁建峰), Wan X-Y(万向元), Wu X-J(吴秀菊), Wang H-L(王海莲), Zhai H-C(翟虎渠), Wan J-M(万建民). Stable expression of QTL for AC and PC of milled rice (Oryza sativa L.) using a CSSL population. Acta Agron Sin (作物学报), 2006, 32(1): 14–19 (in Chinese with English abstract)

[31]Wu C-M(吴长明), Sun C-Q(孙传清), Fu X-L(付秀林), Wang X-K(王象坤), Li Z-C(李自超), Zhang Q(张强). Relationship between quality yield characters or indica-japonica differentiation in rice (Oryza sativa L.). Acta Agron Sin (作物学报), 2003, 29(6): 822–828 (in Chinese with English abstract)
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