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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (03): 514-521.doi: 10.3724/SP.J.1006.2012.00514

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Element Colocalization in Wheat Seed Revealed by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS)

WANG Yun-Xia1,2,YANG Lian-Xin1,*,Walter J. Horst2   

  1. 1 Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China; 2 Institute of Plant Nutrition, Leibniz University of Hannover, 30419 Hannover, Germany
  • Received:2011-07-07 Revised:2011-10-12 Online:2012-03-12 Published:2012-01-04
  • Contact: 杨连新, E-mail: lxyang@yzu.edu.cn

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Abstract: For enhancement of micronutrient concentrations in edible parts of food crops, element uptake and partition in plants, especially in seeds, should be better understanded. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a recently developed technology for examining mineral elements distribution in plant tissues. By using this technique, we quantitatively measured distributions of manganese (Mn), copper (Cu), zinc (Zn), and phosphorus (P) in different parts of wheat seeds. The concentrations of Cu, Zn, and P were the highest in aleurone layer and the lowest in endosperm with the difference of 15, 42, and 33 folds, respectively. The Mn concentration was the highest in embryo, which was 9-fold higher than the lowest concentration in endosperm. The concentration gradients of measured elements were also found in same parts of wheat grain. The concentrations of P, Mn, Cu, and Zn in endosperm close to aleurone layer were higher than those in the middle of wheat seed. Similarly, the element concentrations in scutellum were higher than those in embryo axis. The four elements had similar distribution pattern in wheat seed with a clear synchronization. This phenomenon suggested the colocalization of these elements in wheat seeds. Therefore, the translocations and accumulations of P, Mn, Cu, and Zn in wheat seeds might be closely related to each other, and the finding is useful for wheat biofortification programs in the future.

Key words: Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), Wheat, Seed, Elements, Colocalization

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