Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1710-1715.doi: 10.3724/SP.J.1006.2012.01710

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Modulation of Exogenous Nitric Oxide on Photosystem II Functions in Wheat Seedlings under Drought Stress

SHAO Rui-Xin1,2,XIN Long-Fei1,YANG Qing-Hua1,SHANG-GUAN Zhou-Ping2,*   

  1. 1 Agronomy College of Henan Agriculture University / Key Laboratory of Physiology, Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, China; 2 State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Yangling, 712100, China
  • Received:2011-12-21 Revised:2012-04-20 Online:2012-09-12 Published:2012-07-03
  • Contact: 上官周平, E-mail: shangguan@ms.iswc.ac.cn

PDF

1441

Abstract: Nitric oxide (NO) has emerged as a key molecule involving in tolerating various abiotic stresses that induce physiological responses in plants. To characterize the role of NO in tolerance of photosystem II (PSII) to drought stress induced by polyethylene glycol (PEG), we used sodium nitroprusside (SNP) of 0.1 mmol L-1as exogenous NO donor to study the biologically protective effects of NO at a low concentration on PSII functions in wheat (Triticum aestivum L.) seedlings hydroponically grown under -0.5 MPa PEG stress. Under drought stress, SNP supplement increased the water potential (Ψw) and chlorophyll content, maintained favorable status of electron transport (ФPSII and Fm/Fo) and potential high activity of PSII reaction centers (Fv/Fo) on the first and third days of treatment. The open proportion of PSII reaction centers (qP) and absorbed-light allocation to photochemical reaction(Pr)were higher in the treatment of SNP supplement under drought stress than in only PEG treatment. SNP alleviated drought-induced inhibition to absorbed-energy conversion of open PSII (Fv′/Fm′), safely dissipating excessive light energy in PSII reaction centers. Therefore, NO could modulate the PSII functions in photosynthesis of wheatseedlings hydroponically exposed to PEG stress.

Key words: Nitric oxide, PSII functions, Chlorophyll content, Wheat, Drought stress

[1]Kaoua M E, Serraj R, Benichou M, Hsissou D. Comparative sensitivity of two Moroccan wheat varieties to water stress: the relationship between fatty acids and proline accumulation. Bot Stud, 2006, 47: 51-60

[2]Baquedano F J, Castillo F J. Comparative ecophysiological effects of drought on seedlings of the Mediterranean water-saver Pinus halepensis and water-spenders Quercus coccifera and Quercus ilex. Trees, 2006, 20: 689-700

[3]Maxwell K, Johnson G N. Chlorophyll fluorescence: a practical guide. J Exp Bot, 2000, 51: 659-668

[4]Panda D, Dash P K, Dhal N K, Rout N C. Chlorophyll fluorescence parameters and chlorophyll content in mangrove species grown in different salinity. Gen Appl Plant Physiol, 2006, 32: 175-180

[5]Calatayud A. Chlorophyll a fluorescence as indicator of atmospheric pollutant effects. Toxicol Environ Chem, 2007, 89: 627-639

[6]Golding A J, Johnson G N. Down-regulation of linear and activation of cyclic electron transport during drought. Planta, 2003, 218: 107-114

[7]Duan H G, Yuan S, Liu W J, Xi D H, Qing D H, Liang H G, Lin H H. Effects of exogenous spermidine on photosystem II of wheat seedlings under water stress. J Integr Plant Biol, 2006, 48: 920-927

[8]Szepesi A, Csiszár J, Bajkán S, Gémes K, Horváth F, Erdei L, Deér A K, Simon M L, Tari I. Role of salicylic acid pretreatment on the acclimation of tomato plants to salt- and osmotic stress. Acta Biol Szeged, 2005, 49: 123-125

[9]Dodd I C, Critchley C, Woodall G S, Stewart G R. Photoinhibition in differently colored juvenile leaves of Syzygium species. J Exp Bot, 1998, 49: 1437-1445

[10]Dulai S, Molnár I, Prónay J, Csernák Á, Tarnai R, Láng M M. Effects of drought on photosynthetic parameters and heat stability of PSII in wheat and in Aegilops species originating from dry habitats. Acta Biol Szeged, 2006, 50: 11-17

[11]Baroli I, Melis A. Photoinhibitory damage is modulated by the rate of photosynthesis and by the photosystem II light-harvesting chlorophyll antenna size. Planta, 1998, 205: 288-296

[12]Jasid S, Simontacchi M, Bartoli C G, Puntarulo S. Chloroplasts as a nitric oxide cellular source. Effect of reactive nitrogen species on chloroplastic lipids and proteins. Plant Physiol, 2006, 142: 1246-1255

[13]Bright J, Desikan R, Hancock J T, Weir I S, Neill S J. ABA-induced NO generation and stomatal closure in Arabidopsis are dependent on H2O2 synthesis. Plant J, 2006, 45: 113-122

[14]Oh J I, Kaplan S. Generalized approach to the regulation and integration of gene expression. Mol Microbiol, 2001, 39: 1116-1123

[15]Pfannschmidt T. Chloroplast redox signals: how photosynthesis controls its own genes. Trends Plant Sci, 2003, 8: 33-41

[16]Wendehenne D, Durner J, Klessig D F. Nitric oxide: a new player in plant signaling and defense responses. Curr Opin Plant Biol, 2004, 7: 449-455

[17]Grün S, Lindermayr C, Sell S, Durner J. Nitric oxide and gene regulation in plants. J Exp Bot, 2006, 57: 507-516

[18]Beligni M V, Lamattina L. Nitric oxide stimulates seed germination and de-etiolation, and inhibits hypocotyl elongation, three light-inducible responses in plants. Planta, 2000, 210: 215-221

[19]Zhang Y Y, Wang L L, Liu Y L, Zhang Q, Wei Q P, Zhang W H. Nitric oxide enhances salt tolerance in maize seedlings through increasing activities of proton-pump and Na+/H+ antiport in the tonoplast. Planta, 2006, 224: 545-555

[20]Wang Y S, Yang Z M. Nitric oxide reduces aluminum toxicity by preventing oxidative stress in the roots of Cassia tora L. Plant Cell Physiol, 2005, 46: 1915-1923

[21]Beligni M V, Lamattina L. Nitric oxide in plants: the history is just beginning. Plant Cell Environ, 2001, 24: 267-278

[22]Shao R-X(邵瑞鑫), Shang-Guan Z-P(上官周平). Effects of exogenous nitric oxide donor sodium nitroprusside on photosynthetic pigment content and light use capability of PSII in wheat under water stress. Acta Agron Sin (作物学报), 2008, 34: 818-822 (in Chinese with English abstract)

[23]Strasser R J, Srivastava A, Govindjee. Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria. Photochem Photobiol, 1995, 61: 32-42

[24]Strasser R J, Sivastava A, Tsimilli-Michael M. The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Yunus M, Pathre U, Mohanty P, eds. Probing photosynthesis: mechanisms, regulation, and adaptation. London: Taylor and Francis Press, 2000. pp 445-483

[25]Strasser R J, Srivastava A, Tsimilli-Michael M. Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou, Govindjee G, eds. Advances in Photosynthesis and Respiration. Dordrecht, the Netherlands: KAP Press, 2004. pp 1-42

[26]Sayed O H. Chlorophyll fluorescence as a tool in cereal crop research. Photosynthetica, 2003, 41: 321-330

[27]Wilson K E, Ivanov A G, Öquist G, Grodzinski B, Sarhan F, Huner N P A. Energy balance, organellar redox status, and acclimation to environmental stress. Can J Bot, 2006, 84: 1355-1370

[28]Delledonne M, Xia Y, Dixon R A, Lamb C. Nitric oxide functions as a signal in plant disease resistance. Nature, 1998, 394: 585-588

[29]Scholander P F, Bradstreet E D, Hemmingsen E A, Hammel H T. Sap pressure in vascular plants negative hydrostatic pressure can be measured in plants. Science, 1965, 148: 339-346

[30]Demmig-Adams B, Adams III W W, Barker D H, Logan B A. Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation. Physiol Plant, 1996, 98: 253-264

[31]Neill S, Barros R, Bright J, Desikan R, Hancock J, Harrison J, Morris P, Ribeiro D, Wilson I. Nitric oxide, stomatal closure, and abiotic stress. J Exp Bot, 2008, 59: 165-176

[32]Beligni M V, Lamattina L. Nitric oxide protects against cellular damage produced by methylviologen herbicides in potato plants. Nitric Oxide, 1999, 3: 199-208

[33]Shao R-X(邵瑞鑫), Shang-Guan Z-P(上官周平). Effects of exogenous nitric oxide at different concentrations on the growth and physiology of winter wheat seedlings. Acta Ecol Sin (生态学报), 2008, 28: 302-309 (in Chinese with English abstract)

[34]Torres M A, Jones J D G, Dangl J L. Reactive oxygen species signaling in response to pathogens. Plant Physiol, 2006, 141: 373-378

[35]Tas S, Tas B. Some physiological responses of drought stress in wheat genotypes with different ploidity in Turkiye. World J Ggric Sci, 2007, 3: 178-183

[36]Hetherington A M. Guard cell signaling. Cell, 2001, 107: 711-714

[37]Schroeder J I, Allen G J, Hugouvieux V, Kwak J M., Waner D. Guard cell signal transduction. Annu Rev Plant Physiol Plant Mol Biol, 2001, 52: 627-658

[38]Lesser M P. Oxidative stress in marine environments: biochemistry and physiological ecology. Annu Rev Physiol, 2006, 68: 253-278

[39]Mallick N, Mohn F H, Soeder C J, Grobbelaar J U. Ameliorative role of nitric oxide on H2O2 toxicity to a chlorophycean alga Scenedesmus obliquus. J Gen Appl Microbiol, 2002, 48: 1-7

[40]Akio U, Andre T J, Takashi H, Temhiro T, Tetsuko T. Effects of hydrogen peroxide and nitric oxide on both salt and Heat stress tolerance in rice. Plant Sci, 2002, 163: 515-523

[41]Lamattina L, Mata C G, Graziano M, Pagnussat G. Nitric oxide: the versatility of an extensive signal molecule. Annu Rev Plant Biol, 2003, 54: 109-136

[42]Lazalt A M, Beligni M V, Lamattina L. Nitric oxide preserves the level of chlorophyll in potato leaves infected by Phytophthora infestans. Eur J Plant Pathol, 1997, 103: 643-651

[43]Kitao M, Lei T T, Koike T, Tobita H, Maruyama Y. Higher electron transport rate observed at low intercellular CO2 concentration in long-term drought-acclimated leaves of Japanese mountain birch (Betula ermanii). Physiol Plant, 2003, 118: 406-413

[44]Yang J-D(杨甲定), Zhao H-L(赵哈林), Zhang T-H(张铜会), Yun J-F(云建飞). Effects of exogenous nitric oxide on photochemical activity of photosystem II in potato leaf tissue under non-stress condition. Acta Bot Sin (植物学报), 2004, 46:1009-1014 (in Chinese with English abstract)
[1] Zhai Sheng-Nan, Cao Xin-You, Li Hao-Sheng, Li Ji-Hu, Li Fa-Ji, Liu Jin-Dong, Xia Xian-Chun, Lyu Ying-Ying, Ma Rui-Feng, Wang Ying, Geng Hong-Wei, Liu Jian-Jun. Analysis of the genetic effects of allelic variation at the Pod-A1, Pod-D1, and Pod-2D loci on peroxidase activity in wheat grains [J]. Acta Agronomica Sinica, 2026, 52(6): 1593-1603.
[2] Xi Qian-Hui, Xu Zi-Yuan, Liu Meng-Meng, Wang Hong-Yi, Lang Kai-Lin, Jing Zhen-Hai, Chen Feng, Zhao Lei. Genome-wide association study and candidate gene prediction of grain copper content in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1604-1617.
[3] Mao Jia-Qi, Huang Peng-Yu, Zhao Jia-Jia, Zheng Xing-Wei, Wu Bang-Bang, Hao Yu-Qiong, Qu Fei, Liu Cheng, Ma Peng-Tao, Zheng Jun. Evaluation of powdery mildew resistance in wheat cultivars and molecular detection of resistance genes in Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(6): 1669-1681.
[4] Hu Chuan, Zhao Kai-Nan, Huang Xiu-Li, Wu Jin-Zhi, Ren Kai-Ming, Wang He-Zheng, Fu Guo-Zhan, Huang Ming, Li You-Jun. Effects of tillage methods and nitrogen rates on yield and quality of dryland wheat under one-off irrigation [J]. Acta Agronomica Sinica, 2026, 52(6): 1830-1846.
[5] Chen Xue-Yan, He Hua-Chuan, Li Zheng-Jia, Dong Xin-Pan, Li Ou-Qi, Liu Xiao-Yun, Li Dan-Ping, Chen Zhi-Wei, Liu Guo-Xia, Lyu Sheng-Yuan, Wu Yin-Ying, Zhao Zhen-Dong, Cao Xin-You, Wan He-Ping. Dynamic changes in root organic acid secretion and its transcriptional regulatory mechanisms in ‘Jimai 60’ seedlings under combined salinity-alkalinity stress in hydroponics [J]. Acta Agronomica Sinica, 2026, 52(6): 1859-1875.
[6] Gao Pei-Yang, Li Jin-Xuan, Dong Yu-Kui, Shi Yu, Zhang Zhen, Zhang Yong-Li. Response of wheat tillering and spike formation to nitrogen rate under supplementary irrigation based on soil moisture content [J]. Acta Agronomica Sinica, 2026, 52(6): 1847-1858.
[7] Zhang Xian-Feng, Guo Li-Jian, Li Kang-Chun, Kong Bin-Xue, Liu Yu-Fang, Che Zhuo, Yang De-Long. Identification of the ABHD6 gene family and development of functional markers for grain weight in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1711-1727.
[8] Wang Zhuang-Zhuang, Wu Zi-Jun, Zhang Yong-Xin, Zhang Xin-Yuan, Yuan Li-Xue, Chen Ru-Xue, Liu Shi-Ju, Duan Jian-Zhao, Feng Wei, Wang Tong-Chao, Wang Yong-Hua. Optimized water-nitrogen synergy enhances winter wheat yield and nitrogen use efficiency in clay-loam fluvo-aquic soils of southeastern Henan, China [J]. Acta Agronomica Sinica, 2026, 52(5): 1501-1521.
[9] He Wan-Long, Geng Hong-Wei, Zhang Fei-Fei, Mikereayi·Ababaikere , Luo Zi-Yang, Li Peng-Cheng, Zhou Zhao-Yu, Cheng Yu-Kun. Development of a deep learning-based image recognition system for major wheat diseases [J]. Acta Agronomica Sinica, 2026, 52(5): 1401-1417.
[10] Zhang Zhen, Feng Lian-Jie, Shi Yu, Yu Zhen-Wen, Zhang Yong-Li. Yield formation of wheat with different ear types under water-saving supplementary irrigation conditions [J]. Acta Agronomica Sinica, 2026, 52(5): 1522-1535.
[11] Hou Si-Yu, Wang Guo-Cui, Wei Jin-Gui, Xie Wei-Xin, Yin Wen, Fan Zhi-Long, Chai Qiang, Hu Fa-Long. Effects of green manure combined with chemical nitrogen fertilizer on dry matter accumulation and yield formation of wheat in arid irrigation areas of northwestern China [J]. Acta Agronomica Sinica, 2026, 52(4): 1208-1219.
[12] Shang Yun-Qiu, Zhao Zhu, Chen Huan, Ding Yong-Gang, Qiao Yu-Qiang, Li Wei, Zhang Xiang-Qian, Cao Cheng-Fu, Du Shi-Zhou. Effects of long-term tillage practices on grain-filling and yield formation in rain-fed wheat [J]. Acta Agronomica Sinica, 2026, 52(4): 1236-1250.
[13] Qiao Yu-Xin, Li Cheng-Yue, Kang Xiao-Yu, Zhang Xin-Qi, Jia Shao-Hui, Liu Qian, Cao Ya-Li, Shi Xin-Rui, Hao Xing-Yu, Li Ping. Study on the effects of long-term no-tillage straw mulching on wheat yield improvement in dryland areas based on the APSIM model [J]. Acta Agronomica Sinica, 2026, 52(4): 1181-1192.
[14] Li Can, Zhang Xi-Wei, Zhu Bo-Tao, Zhang Pei-Pei. Functional characterization of wheat GSK kinase TaSK41 and screening for interacting proteins [J]. Acta Agronomica Sinica, 2026, 52(3): 677-687.
[15] Hou Jie, Fu Duo-Duo, Wu Hai-Feng, Hao Yu-Qiong, Zheng Xing-Wei, Wu Bang-Bang, Zhou Kai, Li Xiao-Hua, Zheng Jun, Zhao Jia-Jia. Chromosome diversity and its effects in wheat landraces from Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(3): 746-763.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd