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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (06): 1039-1044.doi: 10.3724/SP.J.1006.2013.01039

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis and Gene Mapping of a degraded hull 2 (dh2) Mutant in Rice (Oryza sativa)

GUO Shuang**, LI Yun-Feng**, REN De-Yong, ZHANG Tian-Quan, and HE Guang-Hua*   

  1. Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing 400716, China
  • Received:2012-10-31 Revised:2013-01-15 Online:2013-06-12 Published:2013-02-19
  • Contact: 何光华, E-mail: hegh@swu.edu.cn

Abstract:

The identification and cloning of novel mutant genes of floral organ in rice play an important role in understanding the molecular genetic mechanisms and molecular signal pathways regulating floral organ development. A rice mutant, degraded hull 2(dh2), which was derived from ethylmethane sulfonate (EMS)-treated Jinhui 10 (Oryza sativa), exhibited defects in hull development. The dh2 floretsdisplayed open hull in whorl 1, however, the rest floral parts in other three whorls had no obvious change. Further analysis indicated that the number of transverse cells decreased, making the lemma or palea narrow, and causing the hull open. The genetic analysis revealed that the dh2 trait is controlled by a single recessive gene. Using the BSA method, the DH2 gene was finally mapped between IND-5 and IND-14 on chromosome 3 with geneticdistances of 0.99 cM and 1.49 cM, respectively. These results are useful for the map-based cloning of DH2 gene, and very important in the studies of floral development biology.

Key words: Rice (Oryza sativa), degraded hull 2 (dh2), Genetic analysis, Gene mapping

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