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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (06): 1027-1034.doi: 10.3724/SP.J.1006.2014.01027


Cloning and Expression Analysis of an F-box Gene (SiFBX) Rapidly Responsive to Drought Stress

IN Heng,YU Qin-Yang,AN Li-Jia,LI Wen-Li   

  1. School of Life Science & Biotechnology, Dalian University of Technology, Dalian 116023, China
  • Received:2013-09-26 Revised:2014-01-12 Online:2014-06-12 Published:2014-03-24


F-box proteins, components of the Skp1-Cullin1-F-box (SCF) protein E3 ubiquitin ligase complex, serve as the variable component responsible for substrate recognition and recruitment in SCF-mediated proteolysis. The anti-drought relative gene of SiFBX (GenBank accession number KC252635.1) which belongs to the F-box super family was cloned from foxtail millet (Setaria italic). The full-length cDNA of SiFBX was 510 bp, which encoded 170 amino acid residues. Protein analysis and structure predication showed that it had a higher proportion of arginine (R), leucine (L), and serine (S) and a lack of trans-membrane domains and signal peptide. Phylogenetic analysis demonstrated that SiFBX had similarity with EID1 and FBW4. Many abiotic stress-related cis-acting elements and transcription factors were discovered in the 1.9 kb upstream region of SiFBX. The results of real-time PCR showed that there were remarkable changes in the expectation level of SiFBX for the treatments with PEG, water-withholding and ABA.

Key words: Setaria italica, Drought response, F-box protein, qRT-PCR

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