Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (08): 1339-1344.doi: 10.3724/SP.J.1006.2013.01339


Expression Characteristics of Key Genes in Lignin Pathway among Different Lodging Resistance Lines of Brassica napus L.

HUANG Jie-Heng**,LI Wei**,QU Cun-Min,LIU Lie-Zhao,XU Xin-Fu,WANG Rui,LI Jia-Na*   

  1. Engineering Research Center of South Upland Agriculture / College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
  • Received:2013-01-10 Revised:2013-04-22 Online:2013-08-12 Published:2013-05-21
  • Contact: 李加纳, E-mail: ljn1950@swu.edu.cn, Tel: 023-68250642


Lignin is related to not only plant disease resistance but also lodging resistance. The lignin content and six key genes (PAL, 4CL, C4H, CCR1, CCR2, and CAD) expression characteristics of the middle stem were analysed at early flowering stage and podding stage of 15 Brassica napus L. varieties with different lodging resistance level. The results indicated that the lignin content generally increased by 28% from early flowering stage to podding stage, especially by 33.5% in the varieties with stronger lodging resistance. Lignin content was significantly different between the varieties with different lodging resistance levels. The expression of key enzyme genes was remarkably different between two stages, and the expression of genes PAL, 4CL, and CCR1 was remarkably different between the varieties with different lodging resistance levels. The expression of gene PAL was significantly correlated with lignin content at both stages, and the expression of gene 4CL was significantly correlated with that of most other genes. Our results suggested that all the genes studied in the paper could control the lignin synthesis, and PAL and 4CL were the most important genes in lignin pathway.

Key words: Lignin, qRT-PCR, Lignin pathway, Lodging resistance

[1]Franker R, Mcmichael C M, Meyer K, Shirley A M, Cusumano J C, Chapple C. Modified lignin in tobacco and poplar plants over-expressing the Arabidopsis gene encoding ferulate-5-hydroxylase. Plant J, 2000, 22: 223–234

[2]Soltani B M, Ehlting J, Douqlas C J. Genetic analysis and epigenetic silencing of At4CL1 and At4CL2 expression in transgenic Arabidopsis. Biotechnology, 2006, 1: 1124–1136

[3]Sibout R, Baucher M, Gatineau M, Doorsselaere J V, Mila I, Pollet B, Maba B, Pilate G, Lapierre C, Boerjan W, Jouanin L. Expression of a poplar cDNA encoding a ferulate-5-hydroxylase/coniferaldehyde5-hydroxylase increases S lignin deposition in Arabidopsis thaliana, Plant Physiol Biochem, 2002, 40: 1087–1096

[4]Zhong R, W. Morrison W H III, Negrel J, Ye Z H. Dual methylation pathway in lignin biosynthesis. Plant Cell, 1998, 10: 2033–2045

[5]Zhong R, W. Morrison W H III, Himmelsbach D S, Farris L, Poole II, Ye Z H. Essential role of cafeoylcoenzyme o-methy l transferase in lignin biosynthesis in woody poplar plants. Plant Physiol, 2000, 124: 563–578

[6]Hu W J, Harding S A, Lung J, Popko J L, Ralph J, Stokke D D, Tsai C J, Chiang V L. Repression of lignin biosynthesis promotes cellulose accumulation and growth in transgenic trees. Nat Biotechnol, 1999, 17: 808–812

[7]Zhao Y L, Lu H, Tao X J, Chen X M, Jiang X N. Modulate the lignin biosynthesis by expression GRP1.8 promoter: anti-4CL1 gene in transgenic tobacco. J Beijing For Univ, 2003, 25(4): 16–20

[8]Guo D J, Chen F, Inoue K, Blount K J, Dixon R A. Down-regulation of caffeic acid 3-o-methyl transferase in transgenic alfalfa: impacts on lignin structure and implications for the biosynthesis of G and S lignin. Plant Cell, 2001, 13: 73–88

[9]Piquemal J, Chamayou S, Nadaud I, Beckert M, Barrière Y, Mila I, Lapierre C, Rigau J, Puigdomenech P, Jauneau A, Digonnet C, Boudet A M, Goffner D, Pichon M, Down-regulation of caffeic acid o-methyl transferase in maize revisited using a transgenic approach. Plant Physiol, 2002, 130: 1675–1685

[10]Thumma B R, Nolan M F, Evans R, Moran G F. Polymorphisms in cinnamoyl CoA reductase (CCR) are associated with variation in microfibril angle in Eucalyptus spp. Genetics, 2005, 171: 1257–1265

[11]Bate N J, Orr J, Ni W, Meromi A, Nadler-Hassar T, Doerner P W, Dixon R A, Lamb C J, Elkind Y. Quantitative relationship between phenylalanine ammonialyase levels and phenylpropanoid accumulation in transgenic tobacco identifies a rate-determining step in natural product synthesis. Proc Natl Acad Sci USA, 1994, 91: 7608–7612

[12]Hu W J, Akiyoshi K, Tsaich J. Compartmentalized expression of two structurally and functionally distinct 4-coumarate: CoA ligase genes in aspen (Populus tremuloides). Proc Natl Acad Sci USA, 1998, 95: 5407–5412

[13]Sewalt V J H, Ni W, Jung H G, Dixon R A. Lignin impact on fiber degradation: increased enzymatic digestibility of genetically engineered tobacco (Nicotiana tabacum) stems reduced in lignin content. J Agric Food Chem, 1997, 45: 1977–1983

[14]Jones L, Ennos A R, Turner S R. Cloning and characterization of irregular xylem4 (irx4): a severely lignin-deficient mutant of Arabidopsis. Plant J, 2001, 26: 205–216

[15]Halpin C, Knight M E, Foxon G A. Manipulation of lignin quality by down-regulation of cinnamyl alcohol dehydrogenase. Plant J, 1994, 6: 339–350

[16]Jiang W-M(姜维梅), Zhang D-Q(张冬青), Xu C-X(徐春霄). Studies on the stem anatomy of Brsssica oil to lodging. J Zhejiang Agric Univ (Agric Life Sci)(浙江大学学报?农业与生命科学版), 2001, 27(4): 439–442 (in Chinese with English abstract)

[17]Thompson D L. Stalk strength of corn as measured by crushing strength and rind thickness. Crop Sci, 1963, 3: 323–329

[18]Zuber M S, Grogan C O. A new technique for measuring stalk strength in corn. Crop Sci, 1961, 1: 378–380

[19]Li X-C(李尧臣), Qi C-K(戚存扣). Lignin content and key gene expression in lignin synthesis of Brassica napus L. with lodging resistance. Jiangsu J Agricl Sci (江苏农业学报), 2011, 27(3): 481–48 (in Chinese with English abstract)

[20]Zhang J(张建), Chen J-C(陈金城), Tang Z-L(唐章林), Wang R(王瑞). Study on the physic-chemical properties of stem as related to lodging in rape. J Southwest Agric Univ (西南农业大学学报), 2006, 28(5): 763–765 (in Chinese with English abstract)

[21]Yang X-D(杨向东). The Study on the Relationship between Lignin Biosynthsis Manipulation and Brassica napus Resistance to Sclerotinia sclerotiorum and Lodging. PhD Dissertation of Chinese Academy of Agricultural Sciences, 2006 (in Chinese with English abstract)

[22]Yang X-D(杨向东), Wang H-Z(王汉中), Hu Z-M(胡赞民), Liu G-H(刘贵华), Shen J-X(沈金雄). The cloning, expression and activity assays of 4-CL cDNA from the Populus tomentosa. J Huazhong Agric Univ (华中农业大学学报), 2006, 25(2): 101–105 (in Chinese with English abstract)

[23]Yamauchi K, Yasuda S, Hamada K, Tsutsumi Y, Fukushima K. Multiform biosynthetic pathway of syringyl lignin in angiosperms. Planta, 2003, 216: 496–501

[24]Bugos R, Chiang V, Campbell W. cDNA cloning, sequence analysis and seasonal expression of lignin-bispecific caffeic acid/5-hydroxyferulic acid O-methyl transferase of aspen. Plant Mol Biol, 1991, 17: 1203–1215

[25]Tamagnone L, Merida A, Parr A, Mackay S, Culianez-Macia FA, Roberts K, Martin C. The AmMYB308 and AmMYB330 transcription factors from antirrhinum regulate phenylpropanoid and lignin biosynthesis in transgenic tobacco. Plant Cell, 1998, 10: 135–154

[26]Vanholme R, Storme V, Vanholme B, Sundin L, Christensen J H, Goeminne G, Halpin C, Rohde A, Morreel K, Boerjan W. A systems biology view of responses to lignin biosynthesis perturbations in Arabidopsis. Plant Cell, 2012, 24: 3506–3529

[27]Baucher M, Monties B, Van M M, Boerjan W. Biosynthesis and genetic engineer in lignin. Crit Rev Plant Sci, 1998, 17: 125–197

[28]Zhang W-H(张文华). Genetic Analysis and QTL Mapping of Lodging Resistance Related Traits in Brassica napus. MS Thesis of Huazhong Agricultural University, 2010 (in Chinese with English abstract)

[1] WANG Xia, YIN Xiao-Yu, Yu Xiao-Ming, LIU Xiao-Dan. Effects of drought hardening on contemporary expression of drought stress memory genes and DNA methylation in promoter of B73 inbred progeny [J]. Acta Agronomica Sinica, 2022, 48(5): 1191-1198.
[2] MENG Ying, XING Lei-Lei, CAO Xiao-Hong, GUO Guang-Yan, CHAI Jian-Fang, BEI Cai-Li. Cloning of Ta4CL1 and its function in promoting plant growth and lignin deposition in transgenic Arabidopsis plants [J]. Acta Agronomica Sinica, 2022, 48(1): 63-75.
[3] LI Zeng-Qiang, DING Xin-Chao, LU Hai, HU Ya-Li, YUE Jiao, HUANG Zhen, MO Liang-Yu, CHEN Li, CHEN Tao, CHEN Peng. Physiological characteristics and DNA methylation analysis under lead stress in kenaf (Hibiscus cannabinus L.) [J]. Acta Agronomica Sinica, 2021, 47(6): 1031-1042.
[4] ZHENG Ying-Xia, CHEN Du, WEI Peng-Cheng, LU Ping, YANG Jin-Yue, LUO Shang-Ke, YE Kai-Mei, SONG Bi. Effects of planting density on lodging resistance and grain yield of spring maize stalks in Guizhou province [J]. Acta Agronomica Sinica, 2021, 47(4): 738-751.
[5] LU Hai, LI Zeng-Qiang, TANG Mei-Qiong, LUO Deng-Jie, CAO Shan, YUE Jiao, HU Ya-Li, HUANG Zhen, CHEN Tao, CHEN Peng. DNA methylation in response to cadmium stress and expression of different methylated genes in kenaf [J]. Acta Agronomica Sinica, 2021, 47(12): 2324-2334.
[6] ZHAO Xiao-Hong,BAI Yi-Xiong,WANG Kai,YAO You-Hua,YAO Xiao-Hua,WU Kun-Lun. Effects of planting density on lodging resistance and straw forage characteristics in two hulless barley varieties [J]. Acta Agronomica Sinica, 2020, 46(4): 586-595.
[7] WANG Kai,ZHAO Xiao-Hong,YAO Xiao-Hua,YAO You-Hua,BAI Yi-Xiong,WU Kun-Lun. Relationship of stem characteristics and lignin synthesis with lodging resistance of hulless barley [J]. Acta Agronomica Sinica, 2019, 45(4): 621-627.
[8] Zuo-Min WANG,Jin LIU,Shi-Chao SUN,Xin-Yu ZHANG,Fei XUE,Yan-Jun LI,Jie SUN. Identification and Expression Analysis of Multidrug and Toxic Compound Extrusion Protein Family Genes in Colored Cotton [J]. Acta Agronomica Sinica, 2018, 44(9): 1380-1392.
[9] Hai-Chao FAN,Wan-Rong GU,De-Guang YANG,Ju-Ping YU,Lin PIAO,Qian ZHANG,Li-Guo ZHANG,Xiu-Hong YANG,Shi WEI. Effect of Chemical Regulators on Physical and Chemical Properties and Lodging Resistance of Spring Maize Stem in Northeast China [J]. Acta Agronomica Sinica, 2018, 44(6): 909-919.
[10] YIN Neng-Wen**,LI Jia-Na**,LIU Xue,LIAN Jian-Ping,FU Chun,LI Wei,JIANG Jia-Yi,XUE Yu-Fei,WANG Jun,CHAI You-Rong*. Lignification Response and the Difference between Stem and Root of Brassica napus under Heat and Drought Compound Stress [J]. Acta Agron Sin, 2017, 43(11): 1689-1695.
[11] CHEN Xue-Ping**,JING Ling-Yun**,WANG Jia,JIAN Hong-Ju,MEI Jia-Qin,XU Xin-Fu,LI Jia-Na,LIU Lie-Zhao*. Correlation Analysis of Sclerotinia Resistance with Lignin Content and Monomer G/S and its QTL Mapping in Brassica napus L. [J]. Acta Agron Sin, 2017, 43(09): 1280-1289.
[12] HU Wen-Ran,FAN Ling,LI Xiao-Rong,XIE Li-Xia,YANG Yang,LI Bo,CHEN Fang-Yuan. Relative Molecular Weight of Lignin in Cotton Fiber [J]. Acta Agron Sin, 2017, 43(06): 940-944.
[13] XIA Min,HU Qun,LIANG Jian,ZHANG Hong-Cheng,GUO Bao-Wei,CAO Li-Qiang,CHEN Hou-Cun. Effect of Variety and Application Amount of Stalk Strengthening Agent on Yield and Lodging Resistance in Rice [J]. Acta Agron Sin, 2017, 43(02): 296-306.
[14] LIU Xing-Bei,WANG Can,HU Dan,YANG Hao,SHE Heng-Zhi,RUAN Ren-Wu,WU Dong-Qian,YI Ze-Lin. Effects of Seed Dressing with Uniconazole Powder on Lodging Resistance of Culm in Common Buckwheat [J]. Acta Agron Sin, 2016, 42(01): 93-103.
[15] XU Jun-Wei,MENG Tian-Yao,JING Pei-Pei,ZHANG Hong-Cheng,LI Chao,DAI Qi-Gen,WEI Hai-Yan,GUO Bao-Wei. Effect of Mechanical-Transplanting Density on Lodging Resistance and Yield in Different Types of Rice [J]. Acta Agron Sin, 2015, 41(11): 1767-1776.
Full text



No Suggested Reading articles found!