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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (08): 1345-1351.doi: 10.3724/SP.J.1006.2013.01345

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Overexpression of Elicitor Protein Encoding Gene Hrip1 Improves Tolerance to Drought and Salt Stresses in Arabidopsis

PENG Xue-Cong,YANG Xiu-Fen,QIU De-Wen,ZENG Hong-Mei,GUO Li-Hua,LIU Zheng*   

  1. Key Laboratory of Pest Management in Crops, Ministry of Agriculture / Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-01-15 Revised:2013-04-22 Online:2013-08-12 Published:2013-05-22
  • Contact: 刘峥, E-mail: liuzheng@caas.cn

Abstract:

Hrip1 is a novel hypersensitive response-inducing protein secreted by necrotrophic fungus,Alternaria tenuissima. The Arabidopsis thaliana transgenic plants overexpressing Hrip1 showed increased tolerance to drought and salt stresses. Five transformed lines of Arabidopsis were confirmed by PCR, RT-PCR and Western blot analysis. T4 progenies of transgenic Arabidopsis lines and the wild-type plants were tested. The results showed the seed germination rate and root length of transgenic plants were all significantly higher than those of wild type plants (P<0.05) under stress conditions. The average seed relative germination rate of transgenic Arabidopsis lines was 32.1% and 77.9%, which were 3.72 and 5.61 times higher than those of wild-type plants in the two days incubation with 50 mmol L−1 NaCl and 75 mmol L−1 mannitol, respectively. The average relative root length in transgenic plants was 81.79% and 93.25%, which were 1.53 and 1.34 times of that in wild type plants at 7th day on the medium containing 150 mmol L−1 NaCl and 50 mmol L−1 mannitol, respectively. Stress tolerances were improved in the three-week old soil-grown seedlings. Survival rate of transgenic plants and wild type plants was 67 % and 42 % under 250 mmol L−1 NaCl stress for 20 days, respectively and 72 % of the transgenic plants and 44 % of wild-type plants were survived at 5th day re-watering after dehydration stress for 25 days. Otherwise, POD and CAT activities were increased in transgenic plants compared with three wild type plants under salt and drought stresses. At 24 h of treatment with 200 mmol L−1 NaCl and 200 mmol L−1 mannitol, the average POD activity in transgenic seedlings was 1.56 and 1.85 times, CAT activity was 1.64 and 1.86 times higher than those in wild plants, respectively. These data indicated thatthe expression of Hrip1 gene positively regulates salt and drought tolerance in transgenic Arabidopsis.

Key words: Protein elicitor, Hrip1, transgenic Arabidopsis, Drought stress, Salt stress

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