Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (08): 1345-1351.doi: 10.3724/SP.J.1006.2013.01345

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Overexpression of Elicitor Protein Encoding Gene Hrip1 Improves Tolerance to Drought and Salt Stresses in Arabidopsis

PENG Xue-Cong,YANG Xiu-Fen,QIU De-Wen,ZENG Hong-Mei,GUO Li-Hua,LIU Zheng*   

  1. Key Laboratory of Pest Management in Crops, Ministry of Agriculture / Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-01-15 Revised:2013-04-22 Online:2013-08-12 Published:2013-05-22
  • Contact: 刘峥, E-mail: liuzheng@caas.cn

Abstract:

Hrip1 is a novel hypersensitive response-inducing protein secreted by necrotrophic fungus,Alternaria tenuissima. The Arabidopsis thaliana transgenic plants overexpressing Hrip1 showed increased tolerance to drought and salt stresses. Five transformed lines of Arabidopsis were confirmed by PCR, RT-PCR and Western blot analysis. T4 progenies of transgenic Arabidopsis lines and the wild-type plants were tested. The results showed the seed germination rate and root length of transgenic plants were all significantly higher than those of wild type plants (P<0.05) under stress conditions. The average seed relative germination rate of transgenic Arabidopsis lines was 32.1% and 77.9%, which were 3.72 and 5.61 times higher than those of wild-type plants in the two days incubation with 50 mmol L−1 NaCl and 75 mmol L−1 mannitol, respectively. The average relative root length in transgenic plants was 81.79% and 93.25%, which were 1.53 and 1.34 times of that in wild type plants at 7th day on the medium containing 150 mmol L−1 NaCl and 50 mmol L−1 mannitol, respectively. Stress tolerances were improved in the three-week old soil-grown seedlings. Survival rate of transgenic plants and wild type plants was 67 % and 42 % under 250 mmol L−1 NaCl stress for 20 days, respectively and 72 % of the transgenic plants and 44 % of wild-type plants were survived at 5th day re-watering after dehydration stress for 25 days. Otherwise, POD and CAT activities were increased in transgenic plants compared with three wild type plants under salt and drought stresses. At 24 h of treatment with 200 mmol L−1 NaCl and 200 mmol L−1 mannitol, the average POD activity in transgenic seedlings was 1.56 and 1.85 times, CAT activity was 1.64 and 1.86 times higher than those in wild plants, respectively. These data indicated thatthe expression of Hrip1 gene positively regulates salt and drought tolerance in transgenic Arabidopsis.

Key words: Protein elicitor, Hrip1, transgenic Arabidopsis, Drought stress, Salt stress

[1]Xiong L Z, Yang Y. Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase. Plant Cell Online, 2003, 15: 745–759



[2]Fujita M, Fujita Y, Noutoshi Y, Takahashi F, Narusaka Y, Shinozaki K Y, Shinozaki K. Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks. Curr Opin Plant Biol, 2006, 9: 436–442



[3]Zhang Y H, Yang X F, Liu Q, Zhang Y L, Zeng H M, Yuan J J, Mao J J. Purification of novel protein elicitor from Botrytis cinerea that induces disease resistance and drought tolerance in plants. Microbiol Res, 2010, 165: 142–151



[4]Zhao M-Z(赵明治), Yang X-F(杨秀芬), Zhang M(张明), Yuan J-J(袁京京), Qiu D-W(邱德文). Purification and Bioactivities of a Protein Growth-activator from Aternaria tenuissima. Chin J Biol Control (中国生物防治), 2007, 23(2): 170–173 (in Chinese with English abstract)



[5]Yang Y Y, Zhang H J, Li G J, Li W, Wang X E, Song F M. Ectopic expression of MgSM1, a Cerato-platanin family protein from Magnaporthe grisea, confers broad-spectrum disease resistance in Arabidopsis. Plant Biotechnol J, 2009, 7(8): 763–777



[6]Qiu D W, Mao J J, Yang X F, Zeng H M. Expression of an elicitor-encoding gene from Magnaporthe grisea enhances resistance against blast disease in transgenic rice. Plant Cell Rep, 2009, 28: 925–933



[7]Kulye M, Liu H, Zhang Y L, Zeng H M, Yang X F, Qiu D W. Hrip1, a novel protein elicitor from necrotrophic fungus, Alternaria tenuissima, elicits cell death, expression of defence-related genes and systemic acquired resistance in tobacco. Plant Cell Environ, 2012, 35: 2104–2120



[8]Clough S J, Bent A F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J, 1998, 16: 735–743



[9]Kobayashi K, Cabral S, Calamante G, Maldonado S. Mentaberry A. Transgenic tobacco plants expressing the potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus. Mol Plant-Microbe Interact, 2001, 14: 1274−1285



[10]Dai X Y, Xu Y Y, Ma Q B, Xu W Y, Wang T, Xue Y B, Chong K. Overexpression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to freezing, drought, and salt stress in transgenic Arabidopsis. Plant Physiol, 2007, 143: 1739–1751



[11]Liu Y-C(刘颖超). Resistance Mechanism and Pathogensis-related Genes ExPression in Different Arabidopsis thaliana Ecotypes to Phytophthora infestans. PhD Dissertation of Agricultural University of Hebei, 2004 (in Chinese with English abstract)



[12]Zhang Y-H(张云华), Qiu D-W(邱德文), Zhang L-J(张立军), Yang X-F(杨秀芬), Zeng H-M(曾洪梅), Yuan J-J(袁京京). Purification and its bioactivity of an activator protein PEBC2 from Botrytis cinerea. Acta Phytophyl Sin (植物保护学报), 2009, 35(2): 123–126 (in Chinese with English abstract)



[13]Tang H-K(唐宏琨), Zeng H-M(曾洪梅). Recent advances in fungal protein elicitor and its transgenic plants. Chin J Biol Control (中国生物防治), 2010, 26(4): 480–485 (in Chinese with English abstract)



[14]Kong X P, Pan J W, Zhang M Y, Xing X, Zhou Y, Liu Y, Li D P, Li D Q. ZmMKK4, a novel group C mitogen‐activated protein kinase kinase in maize (Zea mays), confers salt and cold tolerance in transgenic Arabidopsis. Plant Cell Environ, 2011, 34: 1291–1303



[15]Ali Q, Ashraf M. Induction of drought tolerance in maize (Zea mays L.) due to exogenous application of trehalose: growth, photosynthesis, water relations and oxidative defence mechanism. J Agron Crop Sci, 2011, 197: 258–271



[16]Zhang Y M, Yang J F, Lu S Y, Cai J L, Guo Z F. Overexpressing SgNCED1 in tobacco increases ABA level, antioxidant enzyme activities, and stress tolerance. J Plant Growth Regul, 2008, 27: 151–158

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