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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (06): 940-944.doi: 10.3724/SP.J.1006.2017.00940

• RESEARCH NOTES • Previous Articles    

Relative Molecular Weight of Lignin in Cotton Fiber

HU Wen-Ran,FAN Ling,LI Xiao-Rong,XIE Li-Xia,YANG Yang,LI Bo,CHEN Fang-Yuan   

  1. Xinjiang Key Laboratory of Crop Biotechnology / Laboratory of Molecular Mechanism and Molecular Breeding in Cotton of Xinjiang Academy of Agricultural Sciences / Xinjiang Academy of Agricultural Sciences Biotechnology Institute of Nuclear Technology, Urumqi 830091, China
  • Received:2016-09-12 Revised:2017-03-02 Online:2017-06-12 Published:2017-03-19
  • Supported by:

    This study was supported by the Key Laboratory Projects of Xinjiang Uygur Autonomous Region (2016D03013) and the Talents Engaging in Scientific and Technological Innovations of Xinjiang (2014721025


Molecular weight is one of the important characteristics of polymers, lignin molecular weight and its distribution is one of the contents for studying responses of phenylpropanoid structure, physicochemical characteristics and evaluation of the modified product quality. In this research, the relative molecular weight of lignin extracted respectively by enzyme hydrolysis mild acidolysis and dioxane from mature cotton fibers was studied combined with gel permeation chromatography (GPC). The dioxane lignin (DL) had an average molecular weight (Mw) of 2924 g mol–1, and a number-average molecular weight (Mn) of 2403 g mol–1, which were slightly higher than the enzymatic hydrolysis-mild acidolysis lignin (EMAL, Mw was 2169 g mol–1 and Mn was 1970 g mol–1). The polydispersity coefficient of EMAL was slightly lower than that of DL, showing the higher uniformity in EMAL than in DL. The method of EMAL was more suitable to analyze the relative molecular weight of lignin in cotton fiber. The relative molecular weights of lignin in different cotton varieties, extracted by EMAL, were analyzed, showing that the MW distribution range of lignin was 938–2169 g mol–1, while Mn was 857–1970 g mol–1 and the polydispersity coefficient was 1.09–1.74 (less than 2). The weight average molecular weight had significantly negative correlation with the micronaire value (P<0.05). The number average molecular weight was negatively correlated with fiber length (P<0.05), and the micronaire value (P<0.01), respectively.

Key words: Cotton fiber, Lignin, Relative molecular weight, Enzymatic hydrolysis-mild acidolysis, Gel Permeation Chromatography (GPC)

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