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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (07): 1304-1310.doi: 10.3724/SP.J.1006.2014.01304

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Cloning and Expression Analysis of Two Homologous Genes Coding sn-Glycerol-3-Phosphate Acyltransferase 6 in Brassica napus

LIU Cong1,XIAO Dan-Wang1,HU Xue-Fang1,WU Ke-Bin1,GUAN Chun-Yun1,2,XIONG Xing-Hua1,2,*   

  1. 1 Crop Gene Engineering Key Laboratory of Hunan Province, Hunan Agricultural University, Changsha 410128, China; 2 Oilseed Crops Institute, Hunan Agricultural University, Changsha 410128, China
  • Received:2013-11-16 Revised:2014-04-16 Online:2014-07-12 Published:2014-05-16
  • Contact: 熊兴华, E-mail: ndxiongene@yahoo.com, Tel: 13508487613

Abstract:

A key enzyme sn-glycerol-3-phosphate acyltransferase (GPAT) catalyzes the initial step of TAG biosynthetic pathway, and is involved in many processes including plant growth, development and response to abiotic stresses. In this study, two B. napus GPAT6 homologs were cloned from the leaf of the cultivar xiangyou 15 using RT-PCR, and designated as BnGPAT6-1 and BnGPAT6-2, respectively. The coding DNA sequences (CDS) of two BnGPAT6 genes are 1506 bp in length, encoding two different polypeptides of 501 amino acid residues. The proteins were predicted to consist of a haloacid dehalogenase-like hydrolase domain and a lysophospholipid acyltransferase domain. Multiple sequence alignments and phylogenetic analysis of GPAT proteins showed that BnGPAT6-1 and BnGPAT6-2 share high similarity with GPAT6 genes from B. rapa, B. oleracea, Arabidopsis thaliana,and A. lyrata. Tissue expression amounts of BnGPAT6 genes showed that their mRNA were more abundant in flower than the other organs, and the patterns rise up at first and then down in the developing embryo. The expression of BnGPAT6 genes are inhibited by ABA, while go up simultaneously under drought and 6-BA conditions. In the treatment of salt, the expression of BnGPAT6 genes are uptrend in a short time and then down, and there isn’t obvious change under stress of water logging.

Key words: Brassica napus, sn-glycerol-3-phosphate acyltransferase, Gene cloning, Expression analysis, Abiotic stresses

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