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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (05): 758-767.doi: 10.3724/SP.J.1006.2016.00758

• RESEARCH NOTES • Previous Articles     Next Articles

Meta-analysis and Validation of QTL for Resistance to Gray Leaf Spot in Maize

YAN Wei,LI Yuan,SONG Mao-Xing,ZHANG Kuang-Ye,SUN Ming-Ze,QU Hui,LI Feng-Hai,ZHONG Xue-Mei,ZHU Min,DU Wan-Li,LÜ Xiang-Ling*   

  1. Special Maize Institute, Shenyang Agricultural University, Shenyang 110866, China
  • Received:2015-10-15 Revised:2016-03-02 Online:2016-05-12 Published:2016-03-14
  • Contact: 吕香玲, E-mail: lvxiangling521@126.com; Tel:13664105185 E-mail:yanwei1452@hotmail.com
  • Supported by:

    This study was supported by the NationalNatural Science Foundation of China (31301322) and the Special Talent Appointment in Technology of Liaoning Province (2014215031).

Abstract:

Gray leaf spot (GLS) is one of the most severe leaf diseases of maize worldwide. The breeding progress for resistance to GLS has been seriously hindered by less knowledge about QTL number, QTL intervals and mechanism of GLS. Based on meta-analysis, we conformed 13 consensus QTL regionsfrom 14 articles on resistance to gray leaf spot of maize. One backcross with inbred line 81162 as recurrent parent and inbred line CN165 as donor parent, was used to test the consensus of those 13 QTL regions on the basic of linkage disequilibrium gettingmore than 20 partial separated loci. High level of partial separationindicated that there were QTLs with high effects of resistance to GLS on chromosome 1 and chromosome 4. On chromosome 1, the donor genes closed to markers of umc2227, bnlg1832, umc1243, umc2025, umc1515, umc1297, umc1461 showed the frequency over 50%. Therefore, we inferred that there were a few of highly linked QTLs on chromosome 1. The resistant QTL on chromosome 4 was located between markers bnlg2291 and umc1194. Consequently, this study could lay a foundation for the QTL fine mapping on chromosome 1 and 4 in the donor parent CN165.

Key words: Gray leaf spot, Meta-analysis, Consensus QTLs, Backcross introgression lines, Major QTL

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