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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (02): 277-285.doi: 10.3724/SP.J.1006.2017.00277


Effects of Shading on Leaf Structure and Photosynthetic Fluorescence Characteristics of Soybean seedlings in Maize-Soybean Relay Intercropping System

FAN Yuan-Fang,YANG Feng*,LIU Qin-Lin,CHEN Jun-Xu,WANG Rui,LUO Shi-Ling,YANG Wen-Yu*   

  1. College of Agronomy, Sichuan Agricultural University / Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China.
  • Received:2016-05-17 Revised:2016-09-18 Online:2017-02-12 Published:2016-09-27
  • Contact: 杨峰, E-mail: f.yang@sicau.edu.cn; 杨文钰, E-mail: mssiyangwy@sicau.edu.cn E-mail:851301600@qq.com
  • Supported by:

    The study was supported by the National Natural Science Foundation of China (31571615), the National Key Research and development Program of China (2016YFD0300209), and the Innovation Program of Sichuan Agricultural University ( 04060658).


Shading in relay intercropping system is a major factor affecting the low growing of crop growth and development. This study attempted to compare leaf structure and photosynthetic characteristics of cultivar Nandou 12 under maize-soybean relay intercropping systems. Three treatments were designed including one row maize to one row soybean with 50 cm of row space between maize and soybean (A1); two rows maize to two rows soybean with 40 cm of row space for maize or soybean and 60 cm of row space between maize and soybean (A2), sole cropping of soybean. The result indicated that shading caused the changes of both anatomical structure of leaf and photosynthetic characteristics in maize/soybean relay strip intercropping systems. During the V5 stage of soybean, the PAR density in treatments (A1 and A2) were lower than that in CK by 91.2% and 66.8%, respectively. The content of Chl a of treatments (A1 and A2) was lower than that in CK by 50.24% and 27.9%. The Pn of treatments (A1 and A2) was lower than that in CK by 63.2% and 37.8%. The Fv/Fm, qP, NPQ in leaf of treatments (A1 and A2) were lower than there in CK significantly, Fv'/Fm' and Fq'/Fm' of treatments (A1 and A2) were higher than that in CK. Under light microscope, compared with CK, A1 and A2 treatments decreased palisade tissue thickness and spongy tissue thickness of leaf. The leaf of treatment CK was 2.15 and 1.69 times as thick as that of treatments A1 and A2. In A1 and A2 treatments leaf chloroplast morphology was normal, the number of chloroplasts, granule lamella and osmophilic globule in chloroplast increased; the structure of chloroplast was not damaged; There were few starch grains in chloroplasts; and the structure of cell organelles was normal. Therefore, the different spatial patterns of maize-soybean intercropping system result in different light environments above the soybean canopy, and directly affect soybean leaf structure and photosynthetic fluorescence characteristics.

Key words: Soybean, Intercropping, Photosynthetic fluorescence characteristics, Chloroplast ultrastructure

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