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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (08): 1139-1148.doi: 10.3724/SP.J.1006.2017.01139

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Establishment of 20 PCR DetectionSystem with InDel Molecular Markers in Maize

FENG Bo1,2,**,XU Li-Wen1,**,WANG Feng-Ge1,*,XUE Ning-Ning1,LIU Wen-Bin1,2,YI Hong-Mei1,TIAN Hong-Li1,LV Yuan-Da3,ZHAO Han3,JIN Shi-Qiao4,ZHANG Li-Ke4,YU Rong-Hai2,ZHAO Jiu-Ran1   

  1. 1 Maize Research Center, Beijing Academy of Agriculture & Forestry Sciences / Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing 100097, China; 2 College of Agriculture, Jilin Agricultural University, Changchun 130118, China; 3 Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 4 National Agricultural Technical Extension and Service Center, Beijing 100125, China
  • Received:2016-12-11 Revised:2017-04-20 Online:2017-08-12 Published:2017-04-27
  • Contact: 王凤格，E-mail: gege0106@163.com* E-mail:fengbo02220108@163.com
  • Supported by:

    This research was supported by Grants from National Science and Technology Project from the Ministry,of Science and Technology of China (2015BAD02B02), and Science and Technology Innovation Special Project from Beijing Academy of Agriculture and Forestry Sciences (KJCX20161501).

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Abstract:

In order to improve the detection efficiency with molecular marker , the multiple PCR detection system was constructed. In this study, 10 major materials were used to evaluate the single pair PCR with 238 pairs of InDel primers. According to the software quality evaluation results, product range, and the principle of chromosome uniform distribution, 30 pairs of primers were selected from 192 primers with better performance to form two groups of core primer combinations with amplified products in the range of 80-200 bp and 200-400 bp, There were10 pairs of primers distributing in different chromosomes for each primer combination. Based on core primer combination and comprehensive consideration on chromosome distribution, base fragment, and primers fluorescent color, we established two groups of corn test 20 PCR system and a group of 40 fluorescent capillary electrophoresis.

Key words: Maize, InDel, Multiplex PCR

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