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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (11): 1622-1631.doi: 10.3724/SP.J.1006.2017.01622

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Pyramiding Pi-ta, Pi-b and Wx-mq Genes by Marker-assisted Selection in Rice (Oryza sativa L.)

YAO Shu, CHEN Tao,ZHANG Ya-Dong,ZHU Zhen,ZHAO Qing-Yong,ZHOU Li-Hui,ZHAO Ling,ZHAO Chun-Fang,WANG Cai-Lin*   

  1. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R&D Center,/Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China
  • Received:2017-03-03 Revised:2017-07-23 Online:2017-11-12 Published:2017-08-02
  • Contact: 王才林, E-mail: clwang@jaas.ac.cn, Tel: 025-84390307
  • Supported by:

    This study was supported by the Agricultural Science and Technology Independent Innovation Fund of Jiangsu (CX [12]1003), the Key Research and Development Projects of Jiangsu Province (BE2016370), and the Special Funds for the Construction of Modern Agricultural Industrial Technology System (CARS-01-47).

Abstract:

In recent years, japonica rice variety Nanjing 46, Nanjing 5055 and Nanjing 9108 were planted in a large area in Jiangsu province because of their good eating quality. However, these varieties are not resistant to rice blast, and there is no japonica rice variety with good eating quality and suitable for planting in Huaibei area, Jiangsu province. The goal of modern rice breeding is to develop new varieties with good quality, high yield and multi-resistance. Marker-assisted selection combined with traditional breeding methods can greatly improve breeding efficiency. In this study, the high yield rice variety “Wujing 15” contained two blast resistance genes of Pi-ta and Pi-b was used as gene recipient to be crossed with the rice variety “Nanjing 5055”, which carries the low-amylose content gene Wx-mq. Self-designed multiple PCR system to detect rice blast resistance genes of Pi-ta and Pi-b and Tetra-primer Amplification Refractory Mutation PCR System for Wx-mq were used to detect the target loci in separation generation. Combining with many generations of breeding in the field, resistance identification, and classification of the grain endosperm appearance, a new japonica rice line “Nanjing 0051” with excellent agronomic traits and suitable for planting in Huaibei area, Jiangsu province was bred by multi-generational selection on resistance and endosperm appearance. This study successfully used the three sets of PCR detection system in the marker-assisted selection, providing a rapid selection method in rice breeding also important intermediate materials for quality and disease-resistance.

Key words: Rice, Blast, Low-amylose content, Pi-ta, Pi-b, Wx-mq, Marker-assisted selection

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