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Acta Agron Sin ›› 2018, Vol. 44 ›› Issue (01): 15-23.doi: 10.3724/SP.J.1006.2018.00015

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping of QTLs for Seed Dormancy in Oryza Rufipogon Griff.

SUN Ai-Ling1,WU Hong-Ming1,CHEN Gao-Ming1,ZHANG Tian-Yu1,CAO Peng-Hui1,LIU Shi-Jia1,JIANG Ling1,*,WAN Jian-Min1,2   

  1. 1State Key Loboratory of Crop Genetics and Germplasm Enhancement / Key Laboratory of Biology, Genetics and Breeding of Japonica Rice in Mid-lower Yangtze River, Ministry of Agriculture / Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; 2National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2017-04-06 Revised:2017-09-10 Online:2018-01-12 Published:2017-10-31
  • Supported by:

    This study was supported by the National Key Research and Development Program of China (2016YFD0100101-08), the Agricultural Science and Technology Independent Innovation Fund Project of Jiangsu Province (CX[16]1029), Science and Technology Major Project of Anhui Province (16030701068), and Jiangsu Collaborative Innovation Center for Modern Crop Production.

Abstract:

Seed dormancy of rice is an important agronomic trait related to rice quality and quantity. Studies on genetics and molecular mechanisms of rice seed dormancy are of great significance in breeding fine rice varieties with moderate dormancy. In this research, a set of chromosome segment substitution lines (CSSLs), derived from an indica rice variety 9311 as the recurrent parent and the Oryza rufipogon Griff. as the donor parent, were used to detect the QTLs for dormancy of seeds at different storage dates after harvest. A total of 14 QTLs were detected on chromosomes 3, 4, 5, 6, 7, 10, 11, and 12. The lines with significantly stronger dormancy than the background parent 9311 were selected, showing the more dormancy loci in the lines the more strong dormancy. The F2 population of the cross between Q14 and 9311 was used to verify the QTLs for seed dormancy. A significant dormancy locus qSD-7-2 was mapped on chromosome 7 between the markers RM180 and RM21323, its LOD was 18.49 and the phenotypic variation rate was 33.53%. On this major stable inherited QTL, the allele gene from Oryza rufipogon Griff. significantly increased the dormancy of seeds. These results are available for map-based cloning of major QTLs for seed dormancy, and provide the breeding materials for cultivating appropriate dormant rice varieties.

Key words: Oryza rufipogon Griff., seed dormancy, CSSL, QTL

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