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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (11): 1810-1816.doi: 10.3724/SP.J.1006.2020.04080

• RESEARCH NOTES • Previous Articles    

Salicylic acid improved salinity tolerance of Glycyrrhiza uralensis Fisch during seed germination and seedling growth stages

LI Run-Zhi1(), JIN Qing2, LI Zhao-Hu2, WANG Ye1, PENG Zhen1, DUAN Liu-Sheng1,2,*()   

  1. 1 Beijing University of Agriculture / Beijing Key Laboratory of New Technology in Agricultural Application / National Demonstration Center for Experimental Plant Production Education, Beijing 102206, China
    2 College of Agronomy, China Agricultural University / Engineering Research Center of Plant Growth Regulator, Beijing 100193, China
  • Received:2020-03-29 Accepted:2020-07-02 Online:2020-11-12 Published:2020-07-13
  • Contact: Liu-Sheng DUAN E-mail:lirunzhi7639@163.com;dls@bua.edu.cn

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Abstract:

The present study investigated the effects of salicylic acid treatments on morphology, physiological and biochemical parameters and their relationship to salt stress of G. uralensis Fisch seeds and seedlings. Under the salt stress of 200 mmol L-1 NaCl, the application of 0.5 mmol L-1 salicylic acid on G. uralensis Fisch seeds could significantly promote the elongation of radicle, increase fresh weight of seedlings, reduce the content of malondialdehyde (MDA) and proline in the radicle, and improve the activity of peroxidase (POD). Under the salt stress (i.e. 100 mmol L -1 and 200 mmol L-1 NaCl), the application of 0.5 mmol L-1 salicylic acid on G. uralensis Fisch seedling could reduce MDA and proline content, and increase the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in different degrees. Under salt stress condition, the application of salicylic acid could increase the root glycyrrhizinic acid content. In summary, the application of salicylic acid could improve the tolerance to salt stress by alleviating the inhibition of salt stress on the germination of seeds, increasing the activity of antioxidant enzymes, and reducing the degree of membrane lipid peroxidation.

Key words: salicylic acid, Glycyrrhiza uralensis, salt stress, stress resistance

Table 1

Effect of salicylic acid on the fresh weight of G. uralensis seeds under salt stress (mg)"

处理
Treatment		 处理时间 Treatment time
1 h 6 h 12 h 24 h 72 h
CK0 35.3±1.0 ab 43.6±2.3 a 44.5±2.4 a 48.5±2.6 a 62.4±3.6 a
SA0 36.4±1.5 a 44.7±1.7 a 45.2±2.5 a 49.1±1.9 a 64.1±2.1 a
CK200 32.3±1.6 c 33.2±0.9 c 34.8±2.1 c 37.4±1.0 c 46.8±2.0 c
SA200 34.0±1.4 bc 35.3±1.0 b 38.6±0.5 b 40.3±1.6 b 52.9±1.9 b

Fig. 1

Effect of salicylic acid on the radicle length of G. uralensis seeds under salt stress Treatments are the same as those given in Table 1. Different lowercase letters above the bar mean significant difference at the 0.05 probability level."

Fig. 2

Effects of salicylic acid on the MDA content (A) and proline content (B) in G. uralensis seeds under salt stress Treatments are the same as those given in Table 1. Different lowercase letters above the bar mean significant difference at the 0.05 probability level. MDA: malondialdehyde."

Fig. 3

Effect of salicylic acid on the MDA contents in G. uralensis seedling leaves under salt stress CK0: no SA treatment + no salt stress; SA0: SA treatment + no salt stress; CK100: no SA treatment +100 mmol L-1 NaCl stress; SA100: SA treatment +100 mmol L-1 NaCl stress; CK200: no SA treatment +200 mmol L-1 NaCl stress; SA200: SA treatment +200 mmol L-1 NaCl stress. MDA: malondialdehyde. Different letters above the bar mean significant difference at the 0.05 probability level."

Fig. 4

Effect of salicylic acid on the proline in G. uralensis seedling leaves under salt stress Treatments are the same as those given in Fig. 3. Different lowercase letters above the bar mean significant difference at the 0.05 probability level."

Table 2

Effect of salicylic acid on the antioxidant enzyme activities in G. uralensis seeds under salt stress"

处理
Treatment		 SOD活性
SOD activity (U g-1 FW)		 POD活性
POD activity (OD470 g-1 FW min-1)		 CAT活性
CAT activity (U g-1 FW min-1)
CK0 14±1.3 c 33±1.8 c 2.3±0.2 c
SA0 16±1.2 c 25±1.7 d 2.5±0.2 c
CK200 58±3.6 a 56±2.7 b 4.1±0.3 a
SA200 35±2.0 b 67±3.7 a 3.2±0.2 b

Table 3

Effect of salicylic acid on the antioxidant enzyme activities in G. uralensis seedling leaves under salt stress"

处理
Treatment		 SOD活性
SOD activity (U g-1 FW)		 POD活性
POD activity (OD470 g-1 FW min-1)		 CAT活性
CAT activity (U g-1 FW min-1)
CK0 64.4±4.3 a 9.0±1.1 a 27.3±1.6 b
SA0 64.4±3.6 a 10.6±0.7 a 39.5±1.2 a
CK100 108.2±8.1 a 13.3±0.8 a 27.6±1.2 b
SA100 80.5±5.6 b 11.1±1.3 a 39.9±0.9 a
CK200 130.9±7.6 a 12.3±1.0 b 27.2±2.0 a
SA200 144.3±9.2 a 16.8±0.9 a 32.6±1.5 b

Table 4

Effect of salicylic acid on the glycyrrhizinic acid content in G. uralensis seeds under salt stress (μg g-1 FW)"

处理
Treatment		 1 h 6 h 12 h 24 h 72 h
CK0 0.35±0.02 b 0.45±0.12 ab 0.73±0.09 ab 1.69±0.29 ab 4.62±0.52 b
SA0 0.45±0.03 a 0.65±0.12 a 0.95±0.10 a 1.89±0.36 a 5.81±0.32 a
CK200 0.19±0.01 c 0.25±0.07 b 0.38±0.15 b 0.79±0.04 c 2.64±0.21 c
SA200 0.33±0.03 b 0.43±0.11 ab 0.71±0.19 ab 1.18±0.09 b 3.86±0.45 b

Fig. 5

Effect of salicylic acid on the glycyrrhizinic acid content in G. uralensis seedling roots under salt stress Treatments are the same as those given in Fig. 3. Different lowercase letters above the bar mean significant difference at the 0.05 probability level."

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