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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (5): 661-667.doi: 10.3724/SP.J.1006.2020.94119

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Novel peanut genotype with low behenic acid developed from recombinant inbred lines

Jian-Bin GUO,Li HUANG,Nian LIU,Huai-Yong LUO,Xiao-Jing ZHOU,Wei-Gang CHEN,Bei WU,Dong-Xin HUAI,Xiao-Ping REN,Hui-Fang JIANG()   

  1. Oil Crops Research Institute, China Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, the Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China
  • Received:2019-08-14 Accepted:2019-12-26 Online:2020-05-12 Published:2020-01-16
  • Contact: Hui-Fang JIANG E-mail:peanutlab@oilcrops.cn
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31571713);This study was supported by the National Natural Science Foundation of China(31761143005);This study was supported by the National Natural Science Foundation of China(31801403);This study was supported by the National Natural Science Foundation of China(31871666);the Crop Germplasm Resources Protection Project(2017NWB033);the Plant Germplasm Resources Sharing Platform(NICGR2017-36);the China Agricultural Research System (CARS-13-Germplasm Resource Evaluation for Peanut)

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Abstract:

Peanut is an important source of edible vegetable oil in China, and improving the quality of peanut oil is an important goal in peanut breeding. In this study, the contents of very long-chain saturated fatty acids (arachidic acid, behenic acid, and tetracosanoic acid) were determined by using RIL population containing 140 lines derived from the cross between Zhonghua 10 and ICG12625 with different genetic backgrounds. The content of very long- chain saturated fatty acid in the RILs was 4.27%-7.05%, with an average of 5.54%, the minimum value was 1.46 and 1.63 percentage points lower than that of male and female parents, with reduction rates of 25.27% and 27.62%, respectively. The behenic acid content ranged from 1.86% to 3.37%, with an average of 2.53%. Three lines of QT0002, QT0075, and QT0120 had behenic acid content lower than 2%, which was 0.57, 0.51, and 0.51 percentage points lower than that of Zhonghua10 with the reduction rate of 23.45%, 20.98%, and 20.98%, respectively. A stable QTL located in 13.31-16.34 M of B04 was detected using a linkage map and WinQTLcart software. In this region of 13.31-16.34 M contained 131 prediction genes. The results lay a foundation for the genetic improvement of low behenic acid and high-oleic peanut varieties.

Key words: peanut, recombined inbreed lines (RILs), fatty acid, behenic acid

Fig. 1

Parents’ difference in very long-chain saturated fatty acid "

Fig. 2

Correlationship in very long-chain saturated fatty acid"

Table 1

Statistical analysis of behenic acid in parents and RIL populations"

年份
Year		 亲本 Parent (%) RIL群体 RIL population (%)
中花10号
Zhonghua 10		 ICG12625 最小值
Min		 最大值
Max		 均值
Mean		 标准差
SD		 变异系数
CV		 偏度
Skewness		 峰度
Kurtosis
2015 2.17 2.70 1.80 3.51 2.53 0.31 12.39 0.44 0.34
2016 2.58 3.23 1.90 3.69 2.66 0.37 13.96 0.48 0
2017 2.52 2.81 1.68 3.21 2.40 0.29 11.99 0.08 -0.12

Table 2

Information of other fatty acids of the three lines with low behenic acid (%)"

家系
Line		 山嵛酸
Behenic acid		 棕榈酸
Palmitic acid		 硬脂酸
Stearic acid		 油酸
Oleic acid		 亚油酸
Linoleic acid		 花生酸
Arachidic acid		 花生烯酸
Arachidonic acid		 二十四碳烷酸
Tetracosanoic acid		 超长链饱和脂肪酸
Very long-chain
saturated fatty acid
QT0002 1.86 11.86 2.93 48.49 31.67 1.32 0.88 1.09 4.27
QT0075 1.92 12.73 3.51 42.92 35.78 1.41 0.66 1.13 4.46
QT0120 1.92 11.83 3.69 50.47 28.76 1.54 0.69 1.20 4.65
Zhonghua 10 2.43 10.45 5.30 48.54 29.29 2.09 0.65 1.39 5.90
ICG12625 2.91 13.48 2.48 40.02 37.29 1.32 1.13 1.49 5.73

Fig. 3

Phenotypic distribution of behenic acid in the RIL population across three years"

Table 3

Phenotypic effect of GM2246-2 and AGGS1236 on content of behenic acid in RIL population"

标记
Marker		 基因型
Genotype		 山嵛酸含量Content of behenic acid (%)
2015 2016 2017 均值 Mean
GM2246-2 AA 2.43±0.25 2.57±0.34 2.30±0.25 2.44±0.23
aa 2.67±0.35 2.81±0.39 2.52±0.31 2.66±0.31
aa-AA 0.24** 0.24** 0.22** 0.22**
AGGS1236 AA 2.42±0.28 2.58±0.67 2.31±0.45 2.45±0.25
aa 2.70±0.32 2.78±0.75 2.52±0.31 2.66±0.29
aa-AA 0.28** 0.20** 0.21** 0.21**

Fig. 4

Phenotypic effect of marker on content of VLCSFA in RIL population VLCSFA: very long-chain saturated fatty acid; a: GM2246-2; b: AGGS1236. "

Fig. 5

Analysis of gene ontology enrichments"

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