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Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (5): 1222-1234.doi: 10.3724/SP.J.1006.2022.14060

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Characteristics of endophytic bacterial community structure in roots of sugarcane under different fertilizer applications

XIAO Jian1(), CHEN Si-Yu1, SUN Yan1, YANG Shang-Dong1,*(), TAN Hong-Wei2,*()   

  1. 1Agricultural College, Guangxi University, Nanning 530004, Guangxi, China
    2Guangxi Key Laboratory of Sugarcane Genetic Improvement / Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
  • Received:2021-04-13 Accepted:2021-07-12 Online:2022-05-12 Published:2021-08-03
  • Contact: YANG Shang-Dong,TAN Hong-Wei E-mail:1318513279@qq.com;ysd706@gxu.edu.cn;hongwei_tan@163.com
  • Supported by:
    National Key Research and Development Program of China(2020YFD1000505);National Natural Science Foundation of China(31760368);Guangxi Zhuang Autonomous Region Academy of Agricultural Sciences Guangxi Sugarcane Genetic Improvement Key Laboratory Open Project(16-K-04-01);China Agricuture Research System (Sugarcane Host Cultivation, CARS170206);Guangxi Innovation-Driven Development Special Project (Guike AA17202042);Guangxi Sugarcane Industry Innovation Team Sugarcane Cultivation Post

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Abstract:

To explore the beneficial endophytic bacteria in sugarcanes and their functions, we analyzed the characteristics of endophytic bacterial community structure in roots of sugarcane under different fertilizer applications. At the phylum level, the proportion of Actinobacteria in roots of sugarcanes under different fertilization levels were lower than that in the control. However, the proportion of Chloroflexi in roots of sugarcanes under different fertilization levels applications were higher than that in CK. At the genus level, Haliangium, Acidicapsa, unclassified_f__Ktedonobacteraceae, and unclassified_f__Acidobacteriaceae_ Subgroup_1 were the specific dominant endophytic bacterial genera in root of sugarcanes under conventional fertilization level (3000 kg hm-2, H treatment); while Sphingomonas was the specific dominant endophytic bacterial genus in roots of sugarcanes under fertilization level (1500 kg hm-2, M treatment); Mesorhizobium, norank_f__BIrii41, Glycomyces, norank_f__67-14, Reyranella, unclassified_f__Steroidobacteraceae, unclassified_f__Xanthobacteraceae, norank_f__Roseiflexaceae, and Dongia were the specific dominant endophytic bacterial genus in roots of sugarcanes under the fertilization level (300 kg hm-2, L treatment). On the contrary, Caulobacter, Pseudonocardia, Nocardia, Kitasatospora, norank_f__norank_o__norank_c__Actinobacteria, Oceanibaculum, and Amycolatopsis were the unique dominant endophytic bacterial genera in root of sugarcane without fertilization application (control). And Actinospica, Catenulispora, Acidothermus, Dyella, and norank_f__Xanthobacteraceae were lost in root of sugarcanes under low nitrogen applications. Gene function prediction also revealed that, compared to no-fertilization treatment, both the endophytic bacterial community structure and the metabolic functions of endophytic bacteria in roots of sugarcanes were affected by different fertilizer applications. However, the structures of endophytic bacterial community in roots of sugarcane were significant differences among different fertilization treatments depending on the amounts of fertilizer applications. Compared to conventional fertilization, the yields of sugarcanes, and the structure, metabolic function of endophytic bacteria in roots of sugarcanes were not significantly changed by fertilizer application under 1500 kg hm-2 treatment. In conclusion, the conventional fertilization at the present stage can be substituted by reducing fertilizer application from 3000 kg hm-2 to 1500 kg hm-2 level in sugarcane.

Key words: sugarcane (Saccharum L.), reduced fertilization, endophytic bacteria, high-throughput sequencing

Table 1

Yields of different fertilization levels in sugarcane (t hm-2)"

样本
Sample name		 产量Yield
2018 2019 2020
CK 56.41±2.59 b 58.02±5.32 b 53.95±5.87 b
H 111.09±2.69 a 114.15±5.21 a 108.04±11.22 a
M 107.78±6.92 a 106.60±2.68 a 99.24±5.36 a
L 61.21±4.13 b 64.29±4.05 b 60.79±7.10 b

Table 2

Numbers of different taxonomic levels of endophytic bacteria under different fertilization levels in sugarcane"

样本
Sample		 分类操作单元数量
Number of operational
taxonomic units (OTU)		 不同分类阶元归类数量 Number of different taxonomic categories
门
Phylum		 纲
Class		 目
Order		 科
Family		 属
Genus		 种
Species
CK 406 14 26 73 110 185 276
H 569 18 34 84 138 231 372
M 570 19 31 84 136 234 362
L 641 20 35 93 152 266 419
总计 Total 963 20 40 105 184 338 565

Table 3

Alpha diversity of endophytic bacteria in root of sugarcane under different fertilization levels"

样本
Sample name		 香农指数
Shannon index		 辛普森指数
Simpson index		 ACE指数
ACE index		 Chao1指数
Chao1 index
CK 3.41±0.02 c 0.082±0.012 a 414.08±29.20 b 309.04±19.74 b
H 4.05±0.06 ab 0.039±0.001 b 522.80±9.85 a 538.11±8.83 a
M 4.45±0.12 a 0.030±0.003 b 584.38±34.07 a 545.31±4.66 a
L 4.20±0.06 ab 0.046±0.017 ab 507.29±12.03 a 460.50±82.69 a

Fig. 1

Endophytes bacterial composition in root of sugarcane at phylum level under different fertilization levels Abbreviations of sample are the same as those given in Table 1."

Fig. 2

Endophytes bacterial composition in root of sugarcane at genus level under different fertilization levels Abbreviations of sample are the same as those given in Table 1."

Fig. 3

Venn diagrams of genus and species classification of endophytic bacterial under different fertilization levels in sugarcane Abbreviations of sample are the same as those given in Table 1."

Table 4

Proportion of predicted functional profiles among different samples (Pathway level 1) (%)"

一级功能层 Pathway level 1 H M L CK
代谢Metabolism 25.86 24.16 21.40 28.58
环境信息处理Environmental information processing 24.76 23.43 23.35 28.47
遗传信息处理Genetic information processing 27.02 24.70 21.22 27.05
细胞过程Cellular processes 24.65 24.85 22.42 28.09
人类疾病Human diseases 24.96 24.85 22.96 27.23
有机系统Organismal systems 26.23 23.71 21.30 28.76

Fig. 4

COG function of endophytic bacterial in root of sugarcane at OTU level under different fertilization levels Abbreviations of sample are the same as those given in Table 1."

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