Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (06): 1045-1053.doi: 10.3724/SP.J.1006.2013.01045

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

In silico Expression Profile of Genes in Response to Drought in Peanut

SUN Ai-Qing1,**,ZHANG Jie-Dao2,**,WAN Yong-Shan1,*,LIU Feng-Zhen1,ZHANG Kun1,SUN Li1   

  1. 1 State Key Laboratory of Crop Biology / Shandong Key Laboratory of Crop Biology / College of Agriculture, Shandong Agricultural University, Tai’an 271018, China; 2 College of Life Sciences, Shandong Agricultural University, Tai’an 271018, China
  • Received:2012-07-06 Revised:2013-01-15 Online:2013-06-12 Published:2013-02-19
  • Contact: 万勇善, E-mail: yswan@sdau.edu.cn, Tel: +86 (0)538 8241540

Abstract:

Drought, one of the most important abiotic stresses, usually causes adverse effects on the productivity and quality of crops. In this study, a drought-resistant variety Fenghua 5 was used to analyse leaf cDNA library of peanut treated with 15% PEG by Solexa high-throughput technology, and detect the differentially expressed genes under drought stress. The results of Solexa sequencing indicated the gene expression in peanut transcriptome presented strong nonhomogeneity and redundancy. The sequenced tags less than 10 copies accounted for 73.1% of the total tag types, however its expression level only accounted for 9.0% of the total. A total of 935 differentially expressed genes were screened out based on the reference tags, of which 64.5% were down-regulated. These differentially expressed genes were involved in metabolisms of carbohydrate, protein, nucleic acid, lipid and energy, and secondary metabolism. Gene expression analysis of peanut also showed that nine transcripts related to flavonoid metabolism significantly up-regulated under drought stress, including four encoding flavonoid biosynthesis enzymes, three encoding methyltransferase and two encoding MYB transcription factor. The gene expression analysisusing semi-quantitative RT-PCR assays indicated that AhPAL was induced significantly by 15% PEG treatment for 6 h. The result showed that flavonoids metabolism might play an important role in peanut responding to drought stress.

Key words: Peanut, Drought, High-throughput sequencing, Gene expression profile

[1]Chen K(陈珂), Jiang Q(蒋祺), Lei Y-B(类延宝), Yin C-Y(尹春英). Physiological responses of plants to drought stress. J Anhui Agric Sci (安徽农业科学), 2009, 37(5): 1907–1908 (in Chinese with English abstract)



[2]Sun D-R(孙大容). Peanut Breeding (花生育种学). Beijing: China Agriculture Press, 1998 (in Chinese)



[3]Boyer J S. Plant productivity and environment. Science, 1982, 218: 443–448



[4]Miao J-S(苗锦山), Wang M-L(王铭伦). Advances in effects of water stress on the growth and development in peanut. J Peanut Sci (花生学报), 2003, 32: 368–371 (in Chinese)



[5]Jain A K, Basha S M, Holbrook C C. Identification of drought-responsive transcripts in peanut (Arachis hypogaea L.). Mol Biotechnol Genet, 2001, 4: 502–514



[6]Bhatnagar-Mathur P, Devi M J, Reddy D S, Lavanya M, Vadez V, Serraj R, Yamaguchi-Shinozaki K, Sharma K K. Stress-inducible expression of At DREB1A in transgenic peanut (Arachis hypogaea L.) increases transpiration efficiency under water-limiting conditions. Plant Cell Rep, 2007, 26: 2071–2082



[7]Yan M-L(严美玲), Li X-D(李向东), Lin Y-J(林英杰), Wang L-L(王丽丽), Zhou L-Y(周录英). Effects of drought during seedling stage on physiological traits, yield and quality of different peanut cultivars. Acta Agron Sin (作物学报), 2007, 33(1): 113–119 (in Chinese with English abstract)



[8]Nautiyal P C, Rachaputi N R, Joshi Y C. Moisture-deficit-induced changes in leaf-water content, leaf carbon exchange rate and biomass production in groundnut cultivars differing in specific leaf area. Field Crops Res, 2002, 74: 67–79



[9]Govind G, Harshavardhan V T, Patricia J K, Dhanalakshmi R, Senthil Kumar M, Sreenivasulu N, Udayakumar M. Identification and functional validation of a unique set of drought induced genes preferentially expressed in response to gradual water stress in peanut. Mol Genet Genom, 2009, 281: 591–605



[10]Zhang Z-B(张正斌), Shan L(山仑). Advances of inheritance of physiological characters for drought resistance in crop. Chin Sci Bull (科学通报), 1998, 43(17): 1812–1817 (in Chinese)



[11]Zhang Z-M(张智猛), Wan S-B(万书波), Dai L-X(戴良香), Song W-W(宋文武), Chen J(陈静), Shi Y-Q(石运庆). Estimating and screening of drought resistance indexes of peanut. Chin J Plant Ecol (植物生态学报), 2011, 35(1): 100–109 (in Chinese with English abstract)



[12]Sankar B, Jaleel C A, Manivannan P, Kishorekumar A, Somasundaram R, Panneerselvam R. Effect of paclobutrazol on water stress amelioration through antioxidants and free radical scavenging enzymes in Arachis hypogaea L. Coll Surf B Biointerfaces, 2007, 60: 229–235



[13]Teng X-K(滕晓坤), Xiao H-S(肖华胜). The prospect analysis of gene chip and high throughput technologies for DNA sequencing. Sci China (Ser C) (中国科学C辑), 2008, 38(10): 891–899 (in Chinese)



[14]Sun A-Q(孙爱清), Wan Y-S(万勇善), Liu F-Z(刘风珍), Zhang K(张昆), Qin X-G(秦兴国). Effects of drought stress on photosynthetic characteristics and yield of different peanut varieties. Shandong Agric Sci (山东农业科学), 2010, (10): 32–38 (in Chinese with English abstract)



[15]Zhang Z-M(张智猛), Wan S-B(万书波), Dai L-X(戴良香), Song W-W(宋文武), Chen J(陈静), Miao H-R(苗华荣). Index screening and comprehensive evaluation for drought resistance of peanut varieties at germination stage. J Agric Sci Technol (中国农业科技导报), 2010, 12(1): 85–91 (in Chinese with English abstract)



[16]Audic S, Claverie J M. The significance of digital gene expression profiles. Genome Res, 1997, 7: 986–995



[17]Seki M, Narusaka M, Kamiya A, Ishida J, Satou M, Sakurai T, Nakajima M, Enju A, Akiyama K, Oono Y, Muramatsu M, Hayashizaki Y, Kawai J, Carninci P, Itoh M, Ishii Y, Arakawa T, Shibata K, Shinagawa A, Shinozaki K. Functional annotation of a full length Arabidopsis cDNA collection. Science, 2002, 296: 141–145



[18]Shinozaki K, Yamaguchi-Shinozaki K. Gene networks involved in drought stress response and tolerance. J Exp Bot, 2007, 58: 221–227



[19]Cominelli E, Galbiati M, Vavasseur A, Conti L, Sala T, Vuylsteke M, Leonhardt N, Dellaporta S L, Tonelli C. A guard-cell-specific MYB transcription factor regulates stomatal movements and plant drought tolerance. Curr Biol, 2005, 15: 1196–1200



[20]Rahaie M, Xue G P, Naghavi M R, Alizadeh H, Schenk P M. A MYB gene from wheat (Triticum aestivum L.) is up-regulated during salt and drought stresses and differentially regulated between salt-tolerant and sensitive genotypes. Plant Cell Rep, 2010, 29: 835–844



[21]Akkasaeng C, Tantisuwichwong N, Chairam I, Prakrongrak N, Jogloy S, Pathanothai A. Isolation and identification of peanut leaf proteins regulated by water stress. Pak J Biol Sci, 2007, 10: 1611–1617



[22]Kralj S, van Geel-Schutten G H, Rahaoui H, Leer R J, Faber E J, van der Maarel MJEC, Dijkhuizen L. Molecular characterization of a novel glucosyltransferase from Lactobacillus reuteri strain 121 synthesizing a unique, highly branched glucan with {alpha}-(1->4) and {alpha}-(1->6) glucosidic bonds. Appl Environ Microbiol, 2002, 68: 4283–4291



[23]Han J, Lühs W, Sonntag K, Zähringer U, Borchardt D S, Wolter F P, Heinz E, Frentzen M. Functional characterization of β-ketoacyl-CoA synthase genes from Brassica napus L. Plant Mol Biol, 2001, 46: 229–239



[24]Wang W X, Vinocur B, Shoseyov O, Altman A. Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends Plant Sci, 2004, 9: 244–252



[25]Sautel C F, Ortet P, Saksouk N, Kieffer S, Garin J, Bastien O, Hakimi M A. The histone methylase KMTox interacts with the redox-sensor peroxiredoxin-1 and targets genes involved in Toxoplasma gondii antioxidant defences. Mol Microbiol, 2009, 71: 212–226



[26]Mittler R, Lam E. Identification, characterization, and purification of a tobacco endonuclease activity induced upon hypersensitive response cell death. Plant Cell, 1995, 7: 1951–1962



[27]Seki M, Narusaka M, Abe H, Kasuga M, Yamaguchi-Shinozaki K, Carninic P, Hayashizaki Y, Shinozaki K. Monitoring the expression pattern of 1300 Arabidopsis L-myo-inosi-genes under drought and cold stresses by using a full-length cDNA microarray. Plant Cell, 2001, 13: 61–72



[28]Jang J Y, Kim D G, Kim Y O, Kim J S, Kang H. An expression analysis of a gene family encoding plasma membrane aquaporins in response to abiotic stresses in Arabidopsis thaliana. Plant Mol Biol, 2004, 54: 713–725



[29]Sreenivasulu N, Radchuk V, Strickert M, Miersch O, Weschke W, Wobus U. Gene expression patterns reveal tissue-specific signaling networks controlling programmed cell death and ABA-regulated maturation in developing barley seed. Plant J, 2006, 47: 310–327



[30]Mittler R. Abiotic stress, the field environment and stress combination. Trends Plant Sci, 2006, 11: 15–19



[31]Canada A T, Giannella E, Nguyen T D, Mason R P. The production of reactive oxygen species by dietary flavonols. Free Radic Biol Med, 1990, 9: 441−449



[32]Liu L(刘蕾), Du H(杜海), Tang X-F(唐晓凤), Wu Y-M(吴燕民), Huang Y-B(黄玉碧), Tang Y-X(唐益雄). The roles of MYB transcription factors on plant defense responses and its molecular mechanism. Hereditas (遗传), 2008, 30(10): 1265−1271 (in Chinese with English abstract)



[33]Christie P J, Alfenito M R, Walbot V. Impact of low-temperature stress on general phenylpropanoid and anthocyanin pathways: Enhancement of transcript abundance and anthocyanin pigmentation in maize seedlings. Planta, 1994, 194: 541−549



[34]Xu Z-R(许志茹), Li C-L(李春雷), Cui G-X(崔国新), Sun Y(孙燕). MYB protein of anthocyanin biosynthesis in plant. Plant Physiol Commun (植物生理学通讯), 2008, 44(3): 597−603 (in Chinese with English abstract)



[35]Su H(苏虎), Zhou C-L(周春丽). Influences of different adversity stress on total flavones content in Sarcandra glabra (Thunb) Nakai. J Anhui Agric Sci (安徽农业科学), 2009, 37(17): 7995−7996 (in Chinese with English abstract)



[36]Zhang C-J(张成军), Guo J-Q(郭佳秋), Chen G-X(陈国祥), Xie H-C(解恒才). Effects of high temperature and/or drought on growth and secondary metabotites in Ginkgo biloba leaves. Rural Eco-Environ (农村生态环境), 2005, 21(3): 11−15 (in Chinese with English abstract)



[37]Saito K, Kobayashi M, Gong Z, Tanaka Y, Yamazaki M. Direct evidence for anthocyanidin synthase as a 2-oxoglutarate-dependent oxygenase: molecular cloning and functional expression of cDNA from a red forma of Perilla frutescens. Plant J, 1999, 17: 181−189



[38]Rizhsky L, Liang H, Shuman J, Shulaev V, Davletova S, Mittler R. When defense pathways collide. The response of Arabidopsis to a combination of drought and heat stress. Plant Physiol, 2004, 134: 1683–1696



[39]Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L. MYB transcription factors in Arabidopsis. Trends Plant Sci, 2010, 15: 573−581

[1] CHEN Song-Yu, DING Yi-Juan, SUN Jun-Ming, HUANG Deng-Wen, YANG Nan, DAI Yu-Han, WAN Hua-Fang, QIAN Wei. Genome-wide identification of BnCNGC and the gene expression analysis in Brassica napus challenged with Sclerotinia sclerotiorum and PEG-simulated drought [J]. Acta Agronomica Sinica, 2022, 48(6): 1357-1371.
[2] ZHOU Wen-Qi, QIANG Xiao-Xia, WANG Sen, JIANG Jing-Wen, WEI Wan-Rong. Mechanism of drought and salt tolerance of OsLPL2/PIR gene in rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1401-1415.
[3] YANG Huan, ZHOU Ying, CHEN Ping, DU Qing, ZHENG Ben-Chuan, PU Tian, WEN Jing, YANG Wen-Yu, YONG Tai-Wen. Effects of nutrient uptake and utilization on yield of maize-legume strip intercropping system [J]. Acta Agronomica Sinica, 2022, 48(6): 1476-1487.
[4] LI Yi-Jun, LYU Hou-Quan. Effect of agricultural meteorological disasters on the production corn in the Northeast China [J]. Acta Agronomica Sinica, 2022, 48(6): 1537-1545.
[5] LI Hai-Fen, WEI Hao, WEN Shi-Jie, LU Qing, LIU Hao, LI Shao-Xiong, HONG Yan-Bin, CHEN Xiao-Ping, LIANG Xuan-Qiang. Cloning and expression analysis of voltage dependent anion channel (AhVDAC) gene in the geotropism response of the peanut gynophores [J]. Acta Agronomica Sinica, 2022, 48(6): 1558-1565.
[6] WANG Xing-Rong, LI Yue, ZHANG Yan-Jun, LI Yong-Sheng, WANG Jun-Cheng, XU Yin-Ping, QI Xu-Sheng. Drought resistance identification and drought resistance indexes screening of Tibetan hulless barley resources at adult stage [J]. Acta Agronomica Sinica, 2022, 48(5): 1279-1287.
[7] LI A-Li, FENG Ya-Nan, LI Ping, ZHANG Dong-Sheng, ZONG Yu-Zheng, LIN Wen, HAO Xing-Yu. Transcriptome analysis of leaves responses to elevated CO2 concentration, drought and interaction conditions in soybean [Glycine max (Linn.) Merr.] [J]. Acta Agronomica Sinica, 2022, 48(5): 1103-1118.
[8] WANG Xia, YIN Xiao-Yu, Yu Xiao-Ming, LIU Xiao-Dan. Effects of drought hardening on contemporary expression of drought stress memory genes and DNA methylation in promoter of B73 inbred progeny [J]. Acta Agronomica Sinica, 2022, 48(5): 1191-1198.
[9] XIAO Jian, CHEN Si-Yu, SUN Yan, YANG Shang-Dong, TAN Hong-Wei. Characteristics of endophytic bacterial community structure in roots of sugarcane under different fertilizer applications [J]. Acta Agronomica Sinica, 2022, 48(5): 1222-1234.
[10] DING Hong, XU Yang, ZHANG Guan-Chu, QIN Fei-Fei, DAI Liang-Xiang, ZHANG Zhi-Meng. Effects of drought at different growth stages and nitrogen application on nitrogen absorption and utilization in peanut [J]. Acta Agronomica Sinica, 2022, 48(3): 695-703.
[11] HUANG Li, CHEN Yu-Ning, LUO Huai-Yong, ZHOU Xiao-Jing, LIU Nian, CHEN Wei-Gang, LEI Yong, LIAO Bo-Shou, JIANG Hui-Fang. Advances of QTL mapping for seed size related traits in peanut [J]. Acta Agronomica Sinica, 2022, 48(2): 280-291.
[12] ZHANG Hai-Yan, XIE Bei-Tao, JIANG Chang-Song, FENG Xiang-Yang, ZHANG Qiao, DONG Shun-Xu, WANG Bao-Qing, ZHANG Li-Ming, QIN Zhen, DUAN Wen-Xue. Screening of leaf physiological characteristics and drought-tolerant indexes of sweetpotato cultivars with drought resistance [J]. Acta Agronomica Sinica, 2022, 48(2): 518-528.
[13] WANG Ying, GAO Fang, LIU Zhao-Xin, ZHAO Ji-Hao, LAI Hua-Jiang, PAN Xiao-Yi, BI Chen, LI Xiang-Dong, YANG Dong-Qing. Identification of gene co-expression modules of peanut main stem growth by WGCNA [J]. Acta Agronomica Sinica, 2021, 47(9): 1639-1653.
[14] WANG Jian-Guo, ZHANG Jia-Lei, GUO Feng, TANG Zhao-Hui, YANG Sha, PENG Zhen-Ying, MENG Jing-Jing, CUI Li, LI Xin-Guo, WAN Shu-Bo. Effects of interaction between calcium and nitrogen fertilizers on dry matter, nitrogen accumulation and distribution, and yield in peanut [J]. Acta Agronomica Sinica, 2021, 47(9): 1666-1679.
[15] SHI Lei, MIAO Li-Juan, HUANG Bing-Yan, GAO Wei, ZHANG Zong-Xin, QI Fei-Yan, LIU Juan, DONG Wen-Zhao, ZHANG Xin-You. Characterization of the promoter and 5'-UTR intron in AhFAD2-1 genes from peanut and their responses to cold stress [J]. Acta Agronomica Sinica, 2021, 47(9): 1703-1711.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!