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Acta Agron Sin ›› 2008, Vol. 34 ›› Issue (08): 1403-1408.doi: 10.3724/SP.J.1006.2008.01403

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of Overexpression of Wheat Superoxide Dismutase (SOD) Genes on Salt Tolerant Capability in Tobacco

ZHANG Hai-Na1,LI Xiao-Juan2,LI Cun-Dong1,XIAO Kai1*   

  1. 1 College of Agronomy; 2 College of Life Science, Agricultural University of Hebei, Baoding 071001, Hebei, China
  • Received:2008-01-04 Revised:1900-01-01 Online:2008-08-12 Published:2008-08-12
  • Contact: XIAO Kai

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Abstract: For identification of the function of wheat novel superoxide dismutase (SOD) genes TaSOD1.1 and TaSOD1.2 in improving the salt tolerance, the transgenic tobacco plants with high expression levels of TaSOD1.1 and TaSOD1.2 were generated by the Agrobacterium tumerficians-mediated transformation approach. On NaCl treatment, the transgenic plants showed a better growth performance with less injured and more greenish leaves than control. The SOD activity was largely elevated in the tested transgenic tobacco plants than that in the control, so were the chlorophyll a, b contents, carotenoid content, soluble sugar content and soluble protein content. In the meantime, the MDA content was dramatically declined. Therefore, the transgenic tobacco plants with high expression levels of TaSOD1.1 and TaSOD1.2 could increase the SOD activity, alleviate the over-oxidation de-gree of cytoplasm membrane caused by NaCl stress, and enhance the NaCl tolerant capacity by the up-regulation of exotic target genes at the transcriptional level.

Key words: Superoxide dismutase (SOD) gene, Genetic transformation, Tobacco, Salt tolerant capacity

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