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Genotype-independent transformation technique development and application in maize

YANG Ya-Wen1,2,3,4,6, ZHU Dong-Jie3, PAN Hong3, ZHANG Yun-Tao5,6, XIA Meng-Yin5,6, HAN Bao-Zhu3,6, JIN Min-Liang3, LI Meng-Jiao3, DONG Lu-Peng3, YANG Ning1,2,6, ZHOU Ying5,6, XU Jie-Ting3,6,*, and YAN Jian-Bing1,2,4,6,   

  1. 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China; 2 Hubei Hongshan Laboratory, Wuhan 430070, Hubei, China; 3 XINMI Biotechnology Co., Ltd., Changzhou 213000, Jiangsu, China; 4 Yazhouwan National Laboratory, Sanya 572024, Hainan, China; 5 Institute of Agricultural Sciences of  Xishuangbanna Prefecture of Yunnan Province, Jinghong 666100, Yunnan, China; 6 Yan Jianbing Expert Workstation of Yunnan Province, Jinghong 666100, Yunnan, China
  • Received:2024-03-31 Revised:2024-06-20 Accepted:2024-06-20 Published:2024-07-11
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (32321005), Yan Jianbing Expert Workstation of Yunnan Province (202305AF150111) and the Independent Innovation Fund for Agricultural Science and Technology of Jiangsu Province (CX (21)1003).  

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Abstract:

The genetic transformation of maize inbred lines via Agrobacterium Tumefaciens is highly genotype-dependent. The morphogenetic genes Baby boom (Bbm) and Wuschel2 (Wus2) significantly enhance transformation efficiency and expand the range of amenable inbred lines. However, achieving transgenic seedlings remain challenging for many maize inbred lines, and the underlying mechanism remains unclear. In this study, we found that mixing the target vector with Bbm and Wus2 in a 10:1 ratio facilitates the generation of somatic embryos in most inbred lines. Transient transfection efficiency and the timing of selection are critical factors influencing the formation of somatic embryos and subsequent seedling development. By optimizing infection conditions and delaying selection, we established an efficient and rapid genetic transformation system that is not restricted by genotype. Using this system, we conducted genetic transformation on 131 inbred lines, resulting in successful transgenic plants in 104 of these lines.

Key words: maize, genetic transformation, morphogenic genes, infection efficiency, selection

 [1] 仇焕广, 李新海, 余嘉玲. 中国玉米产业: 发展趋势与政策建议. 农业经济问题, 2021, 7: 4–15.

Chou H G, Li X H, Yu J L. China maize industry: development trends and policy suggestions. Issues Agric Econ, 2021, 7: 4–15 (in Chinese with English abstract).

 [2] Ishida Y, Hiei Y, Komari T. Agrobacterium-mediated transformation of maize. Nat Protoc, 2007, 2: 1614–1621.

 [3] Zhang Q, Zhang Y, Lu M H, Chai Y P, Jiang Y Y, Zhou Y, Wang X C, Chen Q J. A novel ternary vector system united with morphogenic genes enhances CRISPR/Cas delivery in maize. Plant Physiol, 2019, 181: 1441–1448.

 [4] Sylvie De Buck C D W, Montagu M V, Depicker A. Determination of the T-DNA transfer and the T-DNA integration frequencies upon cocultivation of Arabidopsis thaliana root explants. Mol Plant Microbe Interact, 2000, 13: 658–665.

 [5] Lowe K, La Rota M, Hoerster G, Hastings C, Wang N, Chamberlin M, Wu E, Jones T, Gordon-Kamm W. Rapid genotype “independent” Zea mays L. (maize) transformation via direct somatic embryogenesis. In Vitro Cell Dev Biol Plant, 2018, 54: 240–252.

 [6] Boutilier K, Offringa R, Sharma V K, Kieft H, Ouellet T, Zhang L, Hattori J, Liu C M, van Lammeren A A M, Miki B L A, Custers J B M, van Lookeren Campagne M M. Ectopic expression of BABY BOOM triggers a conversion from vegetative to embryonic growth. Plant Cell, 2002, 14: 1737–1749.

 [7] Lowe K, Wu E, Wang N, Hoerster G, Hastings C, Cho M J, Scelonge C, Lenderts B, Chamberlin M, Cushatt J, Wang L, Ryan L, Khan T, Chow-Yiu J, Hua W, Yu M, Banh J, Bao Z, Brink K, Igo E, Rudrappa B, Shamseer P M, Bruce W, Newman L, Shen B, Zheng P, Bidney D, Falco C, Register J, Zhao Z Y, Xu D, Jones T, Gordon-Kamm W. Morphogenic regulators Baby Boom and wuschel improve monocot transformation. Plant Cell, 2016, 28: 1998–2015.

 [8] Keith Lowe G H, Sun X F, Sonriza R G, Paul L, Sam E, Shane A, Kimberly G, Bill G K. Maize LEC1 improves transfromation in both maize and wheat. Plant Biotechnol, 2002: 283–284.

 [9] McFarland F L, Collier R, Walter N, Martinell B, Kaeppler S M, Kaeppler H F. A key to totipotency: Wuschel-like homeobox 2a unlocks embryogenic culture response in maize (Zea mays L.). Plant Biotechnol J, 2023, 21: 1860–1872.

 [10] Liu X, Bie X M, Lin X, Li M, Wang H, Zhang X, Yang Y, Zhang C, Zhang X S, Xiao J. Uncovering the transcriptional regulatory network involved in boosting wheat regeneration and transformation. Nat Plants, 2023, 9: 908–925.

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