Abstract:

To localize, transfer and use interested genes of Haynaldia villosa, a series of wheat-H. villosa alien chromosome lines including translocation lines induced by irradiation have been developed. To identify H. villosa chromosomes or chromosome segments in these materials, 240 STS primer pairs were designed based on EST sequences of rice and wheat, and 34 of them could amplify specific polymorphic bands between common wheat cv. Chinese Spring and H. villosa. These 34 STS primer pairs were further used for screening markers specific to individual chromosomes of H. villosa using 1V to 7V disomic additional lines and their parents. The results showed that the marker CINAU32_-300 could be used to trace chromosome 1V, CINAU33_-280, CINAU34_-510, CINAU35_-1100, CINAU36_-380 and CINAU37_-400 to 2V, CINAU38_-250 to 3V, CINAU39_-950 and CINAU40_-800 to 4V, CINAU41_-745 and CINAU42_-1050 to 5V, CINAU44_-765 and CINAU45_-495 to 7V. These EST-STS markers combined with the two 6V specific markers previously in our institute were used to identify the H. villosa chromosome and chromosome segments of backcrossed generations derived from pollen irradiation. A set of 1V to 7V addition lines and 18 translocation chromosomes involved in different H. villosa chromosomes were identified. Therefore, these chromosome-specific EST-STS markers could be used to detect chromosomes and chromosome segments of H. villosa in common wheat background.

Key words: Wheat, Haynaldia villose, EST-STS marker, Wheat-H.villosa alien chromosome line, Wheat-H.villosa translocation chromosome