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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (1): 11-17.doi: 10.3724/SP.J.1006.2009.00011

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Characterization of a MBF1 Transcriptional Coactivator Factor in Wheat Induced by Stripe Rust Pathogen

  

  • Received:2008-06-23 Revised:2008-07-09 Online:2009-01-12 Published:2008-11-17
  • Contact: KANG Zhen-Sheng

Abstract:

To better understand wheat (Triticum aestivum L.) defense responses to Puccinia striiformis f. sp. tritici, the compatible interaction cDNA library of wheat leaves infected by Puccinia striiformis f. sp. tritici is constructed in our laboratory. A total of 594 genes have been identified and 399 genes have been annotated. On the basis of previous study, a new MBF1 gene was isolated from this cDNA library through in silico cloning and RT-PCR approaches. The gene was tentatively designated as TaMBF1a, whose open reading frame was 429 bp in length and encoded 142 amino acids containing a conserved MBF1 transcription activation domain. The amino acid sequence of TaMBF1a shares 92% identify with OsMBF1 in rice and 80% identify with AtMBF1a in Arabidopsis thaliana. The expression of TaMBF1a gene was at a similar level in leaves, stems, and roots. TaMBF1a protein is possibly a nuclear protein in wheat. The expression patterns results revealed that TaMBF1a was up-regulated in both compatible and incompatible interactions. However, the expression in incompatible interaction was higher than that in compatible interaction. The expression of TaMBF1a was also induced by salicylic acid (SA), ethylene, and abscisic acid (ABA), suggesting that the SA and ethylene pathways might be involved in regulating the host defence responses.

Key words: Stripe rust fungus, Wheat, Multiprotein bridging factor 1(MBF1), In silico cloning, Gene expression

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