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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (1): 18-24.doi: 10.3724/SP.J.1006.2009.00018

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Expression Profiles of Rice Soluble Starch Synthase(SSS) Genes in Response to High Temperature Stress at Filling stage

WEI Ke-Su1,ZHANG Qi-Fang1,CHEN Fang-Min1,*,ZHONG Lian-Jin1,CHEN Neng2,XIE Li-Hong2   

  1. 1College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029,China;2 Rice Product Quality Supervision and Inspection Center,Ministry of Agriculture/China National Rice Research Institute,Hangzhou 310006,China
  • Received:2008-05-14 Revised:2008-07-17 Online:2009-01-12 Published:2008-11-17

Abstract:

The expression responses of eight soluble starch synthase (SSS) isoform genes involved in starch synthesis metabolism in rice endosperms were detected by using two temperature treatments (32℃ and 22℃ for the mean daily temperature, respectively) in chambers and real-time fluorescence quantitative PCR (FQ-PCR). The result showed that the expression patterns of SSS genes in rice endosperms under high temperature stress were isoform-dependent, with relatively high expression levels and the up-regulated patterns for some isoform genes (SSSIIb, SSSIIc, SSSIIIb, and SSSIVa, etc), but relatively low expression levels and the down-regulated patterns for other isoform genes (SSSIIa and SSSIIIa, etc); SSSI and SSSIIIa were highly expressed in rice endosperms during whole filling stages in two temperature treatments , implying that SSSI and SSSIIIa are two major isoform genes of SSS; the expressions of SSSIIb, SSSIIIa, and SSSIVa genes were much more sensitive to high temperature stress compared to those ofSSSI, SSIIc, SSSIIIb, and SSSIVb.

Key words: Real-time fluorescence quantitative PCR(FQ-PCR), Rice (Oryza sativa L.), High temperature, Soluble starch synthase(SSS), Isoform, Expression pattern

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