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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (2): 211-218.doi: 10.3724/SP.J.1006.2009.00211


Establishment of Molecular ID in Soybean Varieties in Heilongjiang

GAO Yun-Lai1,ZHU Rong-Sheng4,**,LIU Chun-Yan1,2,LI Wen-Fu1,JIANG Hong-Wei1,LI Can-Dong1,YAO Bing-Chen1,HU Guo-Hua2,3,*,CHEN Qing-Shan1,*   

  1. 1College of agriculture, Northeast Agricultural University, Harbin 150030,China;2The Crop Research and Breeding Center of Land-Reclamation,Harbin 150090, China; 3The National Research Center of Soybean Engineering and technology, Harbin 150050,China;4College of Science, Northeast Agricural University, harbin 150030, Heilongjiang, China
  • Received:2008-03-15 Revised:2008-09-05 Online:2009-02-12 Published:2008-12-10
  • Contact: HU Guo-Hua


A total of 1 300 cultivars have been bred in China from 1923 to 2005. To well evaluate and use the cultivars, it is necessary to identify scientifically and accurately them by DNA molecular markers due to traditional identification methods can no longer meet requirements. In the paper, 83 cultivars developed by 13 breeding units from six temperature summation zone in Heilongjiang were investigated, 157 polymorphic alleles were detected with 43 SSR primers covering 19 linkage groups of soybean genetic map, for each primer, 2 to 7 allele variations were detected in all cultivars, and 3.65 on average. According to fragment size of allele variation, the data numeralized from the PAGE bands were analyzed by the software ID Analysis 1.0. Nine primers (Satt100, Sat_218, Satt514, Satt551, Satt380, Satt193, Satt191, Satt442, and Sat_084) could be used to identify all 83 soybean cultivars. The PAGE pattern of each cultivar amplified with the 9 primers could be used as a molecular ID of the cultivars. A set of molecular ID were established for 83 soybean cultivars in Heilongjiang.

Key words: Soybean, SSR, Molecular ID

[1] Xiong D-J(熊冬金), Zhao T-J(赵团结), Gai J-Y(盖钧镒). Geo-graphical sources of germplasm and their nuclear and cytoplas-mic contribution to soybean cultivars released during 1923 to 2005 in China. Acta Agron Sin (作物学报), 2008, 34(2): 175-183(in Chinese with English abstract)
[2] She H-D(佘花娣), Chen J-T(陈景堂), Huang Y-Q(黄亚群), Zhu L-Y(祝丽英), Chi S-M(池书敏). Progress on variety identifica-tion of crop using DNA fingerprinting. J Agric Univ Hebei (河北农业大学学报), 2003, 26(suppl): 28-32(in Chinese with English abstract)
[3] Powell W M, Morgante J J, Doyle J W, Mcnicol S V. Genepool variation in genus Glycine subgenus soja revealed by polymor-phic nuclear and chloroplast microsatellites. Genetics, 1996, 144: 793-803
[4] Russell J R, Fuller J D, Macaulau M, Hatz B G, Jahoor A, Powell W, Waugh R. Direct Comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theor Appl Genet, 1997, 9: 714-722
[5] McGregor C E, Lambert C A, Greyling M M, Louw J H, Warnich L. Aocomparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tu-berosum L.) germplasm. Euphytica, 2000, 113: 135-144
[6] Cui Y-H(崔艳华), Qiu L-J(邱丽娟), Chang R-Z(常汝镇), Lü W-H(吕文河). A study of genetic diversity of Huanghuai summer sowing soybean in China. Sci Agric Sin (中国农业科学), 2004, 37(1): 15-22(in Chinese with English abstract)
[7] Wang B(王彪), Chang R-Z(常汝镇), Tao L(陶莉), Guan R-X(关荣霞), Yan L(阎丽), Zhang M-H(张明恢), Feng Z-F(冯忠孚), Qiu L-J(邱丽娟). Identification of SSR primer numbers for ana-lyzing genetic diversity of Chinese cultivated soybean. Mol Plant Breed (分子植物育种), 2003, 1(1): 82-88 (in Chinese with Eng-lish abstract)
[8] Wang L-X(王丽侠), Li Y-H(李英慧), Li W(李伟), Zhu L(朱莉), Guan Y(关媛), Ning X-C(宁学成), Guan R-X(关荣霞), Liu Z-X(刘章雄), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). Establish-ment of a core collection of Changjiang spring growing soybean. Biodivers Sci (生物多样性), 2004, 122(6): 578-385(in Chinese with English abstract)
[9] Xie H(谢华), Guan R-X(关荣霞), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). Genetic diversity of Chinese summer soybean germplasm revealed by SSR markers. Chin Sci Bull (科学通报), 2005, 50(6): 526-535(in Chinese)
[10] Song Q J, Merek L F, Shoemaker R C, Lark K G, Concibido V C, Delannay X, Specht J E, Cregan P B. A new integrated genetic linkage map of the soybean. Theor Appl Genet, 2004, 109: 122-128
[11] Xin J-S(辛景树), Guo J-L(郭景伦), Zhang R-B(张软斌). Com-pare and analysis of a determination report in the variety authen-ticity and the purity of seeds by molecular maker. Seeds (种子), 2005, 24(1): 58-60(in Chinese)
[12] Wang L-X(王立新), Li Y-F(李云伏), Chang L-F(常利芳), Huang L(黄岚), Li H-B(李宏博), Ge L-L(葛玲玲), Liu L-H(刘丽华), Yao J(姚骥), Zhao C-P(赵昌平). Method of ID constitution for wheat cultivars. Acta Agron Sin (作物学报), 2007, 33(10): 1738-1740(in Chinese with English abstract)
[13] Lei T-G(雷天刚), Zhang X-K(张学昆), Lu H(陆合), Li J-N(李加纳), Xu X-F(徐新福), Fan Q-X(范其新), Chen L(谌利). SSR finger printing of yellow seeded rapeseed varieties. J Southwest Agric Univ (西南农业大学学报), 2005, 27(3): 361-364(in Chi-nese with English abstract)
[14] Chen B-Y(陈碧云), Zhang X-D(张冬晓), Wu X-M(伍晓明), Liu F-L(刘凤兰), Lu G-Y(陆光远), Gao G-Z(高桂珍). AFLP analy-sis of 89 cultivars attending the national regional varieties trial. Chin J Oil Crop Sci (中国油料作物学报), 2007, 29(2): 9-14(in Chinese with English abstract)
[15] Zhao H-K(赵洪锟), Li Q-Y(李启云), Wang Y-M(王玉民), Zhuang B-C(庄炳昌). Use of RAPD for identification of soybean cultivars. J Jilin Agric Sci (吉林农业科学), 1998, (4): 6-8(in Chinese with English abstract)
[16] Zhao H-K(赵洪锟), Li Q-Y(李启云), Wang Y-M(王玉民), Zhang M(张明), Zhuang B-C(庄炳昌). DNA fingerprint of the main parents and current varieties of soybean in Jilin province. Chin J Oil Crop Sci (中国油料作物学报), 2000, 22(4): 12-16(in Chi-nese with English abstract)
[17] Shi F(史锋), Zhang Y-Z(张耀洲), Zeng G-W(曾广文). Study on identification of soybean cultivars using RAPD markers. Acta Agric Zhejiangensis (浙江农业学报), 1998, 10(4): 16-19(in Chinese with English abstract)
[18] Chen Q-S(陈庆山), Zhang Z-C(张忠臣), Liu C-Y(刘春燕), Xin D-W(辛大伟), Shan D-P(单大鹏), Qiu H-M(邱红梅), Shan C-Y(单彩云). QTL analysis of major agronomic traits in soybean. Sci Agric Sin (中国农业科学), 2007, 40(1): 41-47(in Chinese with English abstract)
[19] Chen Q-S(陈庆山). Comparative analysis of specialty index and genetic similarity on soybean germplasm with resistance to Cer-cospora sojina Hara. China Biotechnol (中国生物工程杂志), 2005, 25(suppl): 155-158(in Chinese with English abstract)
[20] Xue J(薛津). New soybean breeding examined in Heilongjiang province in 2003. Soybean Sci (大豆科学), 2003, 22(2): 154-159(in Chinese)
[21] Russell J, Fuller J, Young G, Thomas B, Taramino G, Macaulay M, Waugh R, Powell W. Discrimination between barley genotypes using microsatelite markers. Genome, 1997, 40: 442-450
[22] Akagi H, Yokozeki Y, Inagaki A, Fujimura T. Highly polymor-phic microsatellites of rice consist of AT repeats and a classifica-tion of closely related cultivars with these microsatellite loci. Theor Appl Genet, 1997, 94: 61-67
[23] Liu B-Y(刘本英), Cheng H(成浩). Molecular fingerprinting technology and its application in tea germplasm resources. J Tea (茶叶), 2007, 33(4): 198-202(in Chinese with English abstract)
[24] Guo W-Z(郭旺珍), Zhang T-Z(张天真), Pan J-J(潘家驹), He J-L(何金龙). Analysis of RAPD finger printing on main cotton cultivars in China. J Agric Biotechnol (农业生物技术学报), 1996, 4(2): 129-134(in Chinese with English abstract
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