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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (2): 211-218.doi: 10.3724/SP.J.1006.2009.00211

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Establishment of Molecular ID in Soybean Varieties in Heilongjiang

GAO Yun-Lai1,ZHU Rong-Sheng4,**,LIU Chun-Yan1,2,LI Wen-Fu1,JIANG Hong-Wei1,LI Can-Dong1,YAO Bing-Chen1,HU Guo-Hua2,3,*,CHEN Qing-Shan1,*   

  1. 1College of agriculture, Northeast Agricultural University, Harbin 150030,China;2The Crop Research and Breeding Center of Land-Reclamation,Harbin 150090, China; 3The National Research Center of Soybean Engineering and technology, Harbin 150050,China;4College of Science, Northeast Agricural University, harbin 150030, Heilongjiang, China
  • Received:2008-03-15 Revised:2008-09-05 Online:2009-02-12 Published:2008-12-10
  • Contact: HU Guo-Hua

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Abstract:

A total of 1 300 cultivars have been bred in China from 1923 to 2005. To well evaluate and use the cultivars, it is necessary to identify scientifically and accurately them by DNA molecular markers due to traditional identification methods can no longer meet requirements. In the paper, 83 cultivars developed by 13 breeding units from six temperature summation zone in Heilongjiang were investigated, 157 polymorphic alleles were detected with 43 SSR primers covering 19 linkage groups of soybean genetic map, for each primer, 2 to 7 allele variations were detected in all cultivars, and 3.65 on average. According to fragment size of allele variation, the data numeralized from the PAGE bands were analyzed by the software ID Analysis 1.0. Nine primers (Satt100, Sat_218, Satt514, Satt551, Satt380, Satt193, Satt191, Satt442, and Sat_084) could be used to identify all 83 soybean cultivars. The PAGE pattern of each cultivar amplified with the 9 primers could be used as a molecular ID of the cultivars. A set of molecular ID were established for 83 soybean cultivars in Heilongjiang.

Key words: Soybean, SSR, Molecular ID

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