Adzuki bean (Vigna angularis),SSR,Genetic diversity,"/> Screening and Application of SSR Molecular Markers for Genetic Diversity Analysis of Chinese Adzuki Bean germplasm Resources
Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (2): 219-227.doi: 10.3724/SP.J.1006.2009.00219

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Screening and Application of SSR Molecular Markers for Genetic Diversity Analysis of Chinese Adzuki Bean germplasm Resources

XU Ning,CHENG Xu-Zhen,WANG Li-Xia,WANG Su-Hua,LIU Chang-You,SUN Lei,MEI Li   

  1. Institute of Crop Sciences,Chinese Academy of Agricultural Sciences, Beijing 100081,China
  • Received:2008-05-08 Revised:2008-10-15 Online:2009-02-12 Published:2008-12-10
  • Contact: CHENG Xu-Zhen

PDF

2116

Cited

14

Abstract:

Adzuki bean [Vigna angularis (Willd) Ohwi & Ohashi] is a major food legume in China, with special functions in people’ life and agricultural production. In order to evaluate the genetic diversity of adzuki bean germplasm and provide information for gene mining and plant breeding, a total of 375 accessions of adzuki bean core collection were analyzed with 13 pairs of microsatellite (SSR) markers screened from SSR primers of adzuki bean and its relatives. A wide genetic variation was observed in all collections. Four to nineteen alleles per SSR primer pair were detected with an average of 10.23 alleles. The mean of polymorphism information content (PIC) and the percentage of polymorphic loci (P) for the collections in China were 0.561 and 93.523%, respectively. The dendrogram by UPGMA cluster analysis among populations showed that there were correlations between the groups clustered and their putative geographic origins, and the collections from northeast had a closer genetic relationship with that from middle-south region of China. SSR data showed that PIC in Hubei, Shannxi, and Anhui provinces was the first three high, which almost located in the central part of the three dimensions of PCO (principal coordinate analysis). We concluded that Hubei, Shannxi, and Anhui provinces were the origin or diversity center of cultivated adzuki bean in China. The results could be available for collection, conservation, and utilization of adzuki bean germplasm resources.

Key words: Adzuki bean (Vigna angularis)')">Adzuki bean (Vigna angularis), SSR, Genetic diversity

[1] Zheng Z-J(郑卓杰), Wang S-M(王述民), Zong X-X(宗绪晓). China Food Legume Science(中国食用豆类学). Beijing: China Aquaculture Press, 1997. pp 173-195(in Chinese)
[2] Wang S-M(王述民), Hu Y-K(胡英考), Hu J-P(胡家蓬). Study on genetic diversity of Adzuki bean (Vigna angularis Willd. Ohwi & Ohashi) germplasm based on RAPD markers. J Plant Genet Resour (植物遗传资源学报), 2002, 3(1): 14-19(in Chi-nese with English abstract)
[3] Jin W-L(金文林), Guo B(郭蓓), Wen Z-X(文自翔), Li N(李囡), Pu S-J(濮绍京), Zhao B(赵波). Study on germplasm resources of wild Adzuki bean (V. angularis var. nipponensis) based on RAPD analysis. Acta Agric Boreali-Sin (华北农学报), 2004, 19(2): 28-31(in Chinese with English abstract)
[4] Jin W-L(金文林), Wen Z-X(文自翔), Pu S-J(濮绍京), Zhao B(赵波). Genetic diversity and evolution of Adzuki bean (Vigna angularis) germplasm resources based on RAPD markers. Sci Agric Sin (中国农业科学), 2005, 38(2): 241-249(in Chinese with English abstract)
[5] Mimura M, Yasuda K, Yamaguchi H. RAPD variation in wild, weedy and cultivated azuki beans in Asia. Genet Resour Crop Evol, 2000, 47: 603-610
[6] Xu R Q, Tomooka N, Vaughan D A, Doi K. The Vigna angularis complex: Genetic variation and relationships revealed by RAPD analysis, and their implications for in situ conservation and do-mestication. Genet Resour Crop Evol, 2000, 47: 123-134
[7] Wang S-M(王述民), Zhang C-H(张赤红). Genetic diversity of Adzuki bean (Vigna angularis Willd. Ohwi & Ohashi) germ-plasm revealed by AFLP markers. J Plant Genet Resour (植物遗传资源科学), 2002, 3(3): 1-5(in Chinese with English abstract)
[8] Zong X-X(宗绪晓), Vaughan D, Kaga A, Tomooka N, Wang X-W(王新望), Guan J-P(关建平), Wang S-M(王述民). Prelimi-nary study on geographical distribution and evolutionary rela-tionships between cultivated and wild Adzuki bean (Vigna angu-laris var. angularis and var. nipponensis) by AFLP analysis. Sci Agric Sin (中国农业科学), 2003, 36(4): 367-374(in Chinese with English abstract)
[9] Zong X-X(宗绪晓), Vaughan D, Kaga A, Tomooka N, Wang X-W(王新望), Guan J-P(关建平), Wang S-M(王述民). Genetic diversity in Vigna angularis revealed by AFLP analysis. Acta Agron Sin (作物学报), 2003, 29(4): 562-568(in Chinese with English abstract)
[10] Su S-Q(粟生群), Rong T-Z(荣廷昭), She Y-H(佘跃辉), Liu J(刘坚). Genetic diversity in cultivated azuki bean (Vigna angularis var. angularis) germplasm resources revealed by AFLP markers. J Sichuan Agric Univ (四川农业大学学报), 2005, 23(2): 156-162(in Chinese with English abstract)
[11] Xu R Q, Tomooka N, Vaughan D A. AFLP markers for charac-terizing the Azuki bean complex. Crop Sci, 2000, 40: 808-815
[12] Yoon M S, Lee J, Kim C Y, Baek H J. Genetic relationships among cultivated and wild Vigna angularis Willd. Ohwi et Oha-shi and relatives from Korea based on AFLP markers. Genet Re-sour Crop Evol, 2007, 54: 875-883
[13] Wang X W, Kaga A, Tomooka N, Vaughan D A. The develop-ment of SSR markers by a new method in plants and their appli-cation to gene flow studies in Adzuki bean (Vigna angularis Willd. Ohwi & Ohashi). Theor Appl Genet, 2004, 109: 352-360
[14] Li M-F(李明芳), Zheng X-Q(郑学勤). Research progress of methods of SSR primers development. Hereditas (遗传), 2004, 26(5): 769-776(in Chinese with English abstract)
[15] Rod P, Simon G, Wes K, Michele M, Antoni R. Cross-species amplification of soybean (Glycine max) simple sequence repeats (SSRs) within the genus and other legume genera: implications for the transferability of SSRs in plants. Mol Biol Evol, 1998, 15: 1275-1287
[16] Dikshit H K, Jhang T, Singh N K, Koundal K R, Bansal K C, Chandra N, Tickoo J L, Sharma T R. Genetic differentiation of Vigna species by RAPD, URP and SSR markers. Biol Plant, 2007, 51: 451-457
[17] Xu N(徐宁), Cheng X-Z(程须珍), Wang S-H(王素华), Wang L-X(王丽侠), Zhao D(赵丹). Establishment of an Adzuki bean (Vigna angularis) core collection based on geographical distribu-tion and phenotypic data in China. Acta Agron Sin (作物学报), 2008, 34(8): 1366-1373(in Chinese with English abstract)
[18] Bassam B, Gaetano A J, Gresshoff P M. Fast and sensitive silver staining of DNA in polycrylamide gels. Anal Biol, 1991, 196: 80-83
[19] Van Toai T T, Peng J, Martin S. Optimization of silver staining AFLP technique for Soybean. Soybean Genet Newsl, 1996, 3: 206-209
[20] Gwag J G, Chung J W, Chung H K, Lee J H, Ma K H, Dixit A, Park Y J, Cho E G, Kim T S, Lee S H. Characterization of new microsatellite markers in mungbean, Vigna radiate L. Mol Ecol Notes, 2006, 6: 1-3
[21] Kumar S V, Gtan S G, Quah S C, Yusoff K. Isolation and charac-terization of seven tetranucleotide microsatellite loci in mung-bean, Vigna radiate. Mol Ecol Notes, 2002, 2: 293-295
[22] Li C D, Christian A F, Benjamin U, Singh B B, Scoles G J. Determining genetic similarities and relationships among cowpea breeding lines and cultivars by microsatellite markers. Crop Sci, 2001, 41: 189-197
[23] Zhang X-Y(张晓燕). The gene pool tracing and genetic diversity assessment of common bean (Phaseolus vulgaris L.) germplasm resources from China. Ph.D Dissertation of Chinese Academy of Agricultural Sciences, 2007(in Chinese with English abstract)
[24] Nei M. Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA, 1973, 70: 3321-3323
[25] Yeh F Y, Boyle R, Ye T, Mao Z. POPGENE, the user-friendly shareware for population genetic analysis, version 1.31. Molecu-lar Biology and Biotechnology Centre, Alberta: University of Alberta, 1997
[26] Rohlf F. NTSYS-pc Numerical Taxonomy System, Exeter Pub-lishing. NY: Setauket, 2002
[27] Yu P(余萍), Li Z-C(李自超), Zhang H-L(张洪亮), Li D-Y(李道远), Wang M-X(王美兴), Sun J-L(孙俊立), Wang X-K(王象坤). Genetic diversity of common wild rice (Oryza rufipogon Griff.) by using SSR markers and phenotypic traits in Guangxi province. Acta Genet Sin (遗传学报), 2004, 31(9): 934-940(in Chinese with English abstract)
[28] Zhou Y-Q(周延清). The Application of DNA Molecular Markers in Study on Plants (分子标记技术在植物研究中的应用). Bei-jing: Chemical Industry Press, 2005. pp 131-143 (in Chinese)
[29] Chen J-F(陈军方), Ren Z-L(任正隆), Gao L-F(高丽锋), Jia J-Z(贾继增). Developing new SSR markers from EST of wheat. Acta Agron Sin (作物学报), 2005, 31(2): 154-158(in Chinese with English abstract)
[30] Liu C-Y(刘长友), Cheng X-Z(程须珍), Wang S-H(王素华), Wang L-X(王丽侠), Sun L(孙蕾), Mei L(梅丽), Xu N(徐宁). Screening of SSR and STS markers for genetic diversity analysis of mungbean germplasm. J Plant Genet Resour (植物遗传资源科学), 2007, 8(3): 298-302(in Chinese with English abstract)
[31] Xu Y-H(徐雁鸿), Guan J-P(关建平), Zong X-X(宗绪晓). Ge-netic diversity analysis of cowpea germplasm resources by SSR. Acta Agron Sin (作物学报), 2007, 33(7): 1206-1209(in Chinese with English abstract)
[32] Wang S-M(王述民). The Core Collection Establishment and Its Genetic Diversity Assessment and Classification of Adzuki Bean
[Vigna angularis (Willd) Ohwi & Ohashi] Germplasm Resources. PhD Dissertation of China Agricultural University, 2001(in Chi-nese with English abstract)
[1] Zhang Ying-Xing, Bheel Chander Kumar, Song Yu-Zhen, Wang Yue, Cao Yue, Khound Rituraj, Santra Dipak Kumar, Cao Xiao-Ning, Wang Rui-Yun. Screening and phenotypic characterization of EMS-induced mutants with elite agronomic traits in broomcorn millet [J]. Acta Agronomica Sinica, 2026, 52(5): 1388-1400.
[2] Tian Chun-Yan, Lu Xin, Wu Cai-Wen, Xu Chao-Hua, Liu Jia-Yong, Bian Xin, Tao Lian-An. Genetic diversity analysis and breeding potential evaluation of innovative sugarcane germplasm based on fluorescent SSR [J]. Acta Agronomica Sinica, 2026, 52(4): 1057-1072.
[3] WANG Hao-Chen, WANG Ke-Jing, HAN Juan, LI Xiang-Hua. Spatial genetic structure characteristics of natural populations of Glycine tomentella in two representative habitats in the southeast coast, China: a study of intrapopulation sampling strategy [J]. Acta Agronomica Sinica, 2025, 51(11): 2875-2885.
[4] ZHENG Dong, ZHOU Xian-Li, TENG Chang-Cai, HOU Wan-Wei, ZHANG Hong-Yan, LIU Yu-Jiao. Genetic relationship analysis and fingerprints construction of faba bean varieties in Qinghai province based on SSR markers [J]. Acta Agronomica Sinica, 2025, 51(1): 79-90.
[5] KUANG Bo-Wen, WEI Ni, LIU Jin-Dian, CHEN Mei-Yan, MAO Xing-Jie, DUAN Wei-Xing, YANG Xi-Ping. Development of SSR markers and database based on genomes of sugarcane and its relatives [J]. Acta Agronomica Sinica, 2025, 51(1): 103-116.
[6] DING Yi-Bing, XIN Xu-Xia, FENG Zhi-Zun, GUO Juan, CAO Yue, CHEN Xi-Ming, WANG Xiao-Dan, CAO Xiao-Ning, SANTRA Dipak K, CHEN Ling, QIAO Zhi-Jun, WANG Rui-Yun. Core germplasm identification of proso millet in spring sowing area of Northeast China using fluorescent microsatellite markers [J]. Acta Agronomica Sinica, 2024, 50(7): 1728-1739.
[7] DING Yi-Bing, XIN Xu-Xia, FENG Zhi-Zun, CAO Yue, GUO Juan, Dipak K SANTRA, WANG Rui-Yun, CHEN Xi-Ming. Core germplasm and DNA molecular identity card of proso millet in Northeast Spring sowing region in China [J]. Acta Agronomica Sinica, 2024, 50(5): 1181-1192.
[8] TIAN Chun-Yan, BIAN Xin, LANG Rong-Bin, YU Hua-Xian, TAO Lian-An, AN Ru-Dong, DONG Li-Hua, ZHANG Yu, JING Yan-Fen. Association analysis of three breeding traits with SSR markers and exploration of elite alleles in sugarcane [J]. Acta Agronomica Sinica, 2024, 50(2): 310-324.
[9] CHEN Tian, LI Yu-Ying, RONG Er-Hua, WU Yu-Xiang. Character identification and floral organ transcriptome analysis on artificial allotetraploids of Gossypium hirsutum L. [J]. Acta Agronomica Sinica, 2024, 50(2): 325-339.
[10] CHEN Zhi-Kai, ZHOU Xian-Li, ZHANG Hong-Yan, TENG Chang-Cai, HOU Wan-Wei. SSR association analysis of the protein content of 320 faba bean germplasms [J]. Acta Agronomica Sinica, 2024, 50(11): 2775-2786.
[11] CAO Yue, Zhang Li-Yuan, XIN Xu-Xia, FENG Zhi-Zun, GUO Juan, WANG Xiao-Dan, CAO Xiao-Ning, SANTRA Dipak K, CHEN Ling, QIAO Zhi-Jun, WANG Rui-Yun. Construction of DNA molecular identity card of proso millet in Ningxia based on fluorescent SSR [J]. Acta Agronomica Sinica, 2024, 50(11): 2699-2711.
[12] GUO Juan, XIN Xu-Xia, FENG Zhi-Zun, CAO Yue, WANG Xiao-Dan, CAO Xiao-Ning, SANTRA Dipak K, CHEN Ling, QIAO Zhi-Jun, WANG Rui-Yun. Molecular identification of broomcorn millet germplasm resources in Shaanxi based on SSR [J]. Acta Agronomica Sinica, 2024, 50(10): 2643-2653.
[13] WANG Qian, ZHANG Li-Yuan, XU Yue, LI Hai, LIU Shao-Xiong, XUE Ya-Peng, LU Ping, WANG Rui-Yun, LIU Min-Xuan. High motif EST-SSR markers development and genetic diversity evaluation for 200 core germplasms in proso millet [J]. Acta Agronomica Sinica, 2023, 49(8): 2308-2318.
[14] CHEN Yi-Hang, TANG Chao-Chen, ZHANG Xiong-Jian, YAO Zhu-Fang, JIANG Bing-Zhi, WANG Zhang-Ying. Construction of core collection of sweetpotato based on phenotypic traits and SSR markers [J]. Acta Agronomica Sinica, 2023, 49(5): 1249-1261.
[15] LI Ying, LIU Hai-Cui, SHI Lyu, SHI Xiao-Xu, HAN Xiao, LIU Jian, WEI Ya-Feng. Genetic diversity and population structure analysis of naked barley germplasm resources in Jiangsu province [J]. Acta Agronomica Sinica, 2023, 49(10): 2687-2697.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd