Adzuki bean (Vigna angularis),SSR,Genetic diversity,"/> Screening and Application of SSR Molecular Markers for Genetic Diversity Analysis of Chinese Adzuki Bean germplasm Resources
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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (2): 219-227.doi: 10.3724/SP.J.1006.2009.00219

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Screening and Application of SSR Molecular Markers for Genetic Diversity Analysis of Chinese Adzuki Bean germplasm Resources

XU Ning,CHENG Xu-Zhen,WANG Li-Xia,WANG Su-Hua,LIU Chang-You,SUN Lei,MEI Li   

  1. Institute of Crop Sciences,Chinese Academy of Agricultural Sciences, Beijing 100081,China
  • Received:2008-05-08 Revised:2008-10-15 Online:2009-02-12 Published:2008-12-10
  • Contact: CHENG Xu-Zhen

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Abstract:

Adzuki bean [Vigna angularis (Willd) Ohwi & Ohashi] is a major food legume in China, with special functions in people’ life and agricultural production. In order to evaluate the genetic diversity of adzuki bean germplasm and provide information for gene mining and plant breeding, a total of 375 accessions of adzuki bean core collection were analyzed with 13 pairs of microsatellite (SSR) markers screened from SSR primers of adzuki bean and its relatives. A wide genetic variation was observed in all collections. Four to nineteen alleles per SSR primer pair were detected with an average of 10.23 alleles. The mean of polymorphism information content (PIC) and the percentage of polymorphic loci (P) for the collections in China were 0.561 and 93.523%, respectively. The dendrogram by UPGMA cluster analysis among populations showed that there were correlations between the groups clustered and their putative geographic origins, and the collections from northeast had a closer genetic relationship with that from middle-south region of China. SSR data showed that PIC in Hubei, Shannxi, and Anhui provinces was the first three high, which almost located in the central part of the three dimensions of PCO (principal coordinate analysis). We concluded that Hubei, Shannxi, and Anhui provinces were the origin or diversity center of cultivated adzuki bean in China. The results could be available for collection, conservation, and utilization of adzuki bean germplasm resources.

Key words: Adzuki bean (Vigna angularis)')">Adzuki bean (Vigna angularis), SSR, Genetic diversity

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