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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (5): 786-794.doi: 10.3724/SP.J.1006.2009.00786

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Characterization of a Novel Wheat Glycoside Hydrolase Gene TaGlc2 Induced by Powdery Mildew Pathogen(Erysiphe graminis) Infection

Ramesh N PUDAKE**,XIN Ming-Ming**,YIN Yu-Jing1,XIE Chao-Jie,NI Zhong-Fu,SUN Qi-Xin*   

  1. State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization,Ministry of Education/Key Laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University,Beijing 100193,China
  • Received:2008-10-07 Revised:2009-02-17 Online:2009-05-12 Published:2009-03-20
  • Contact: SUN Qi-Xin E-mail:qxsun@cau.edu.cn
  • Supported by:

    The work was supported by the National High Technology Research and Development Program of China(2006AA10A104),Fok Ying-Tung Education Foundation(94021),and Natural Science Foundation of Beijing, China(6061003 and 30871528)

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Abstract:

Fungal diseases cause serious yield losses of wheat worldwide. Up to date, numerous genes involved in the wheat-pathogen response have been identified. These include pathogenesis related (PR) genes and antifungal hydrolases such as glucanase and chitinase genes. Recently, there has been increasing number of studies providing evidence of the potential involvement of 1,3-β-glucanase in defense against fungal infection. In this study we identified a cDNA encoding a 1,3-β-glucanase, designated TaGlc2, from wheat cDNA library. The deduced peptide sequence of TaGlc2 is similar to a glycoside hydrolase family 17. Using real time PCR, the expression pattern of TaGlc2 in wheat seedlings inoculated with powdery mildew pathogen (Erysiphe graminis) was determined. The results showed that TaGlc2 is inducible in response to fungal infection. The 5' genomic region of TaGlc2 was isolated and it contains some cis-elements which are reported to be involved in pathogenesis response.

Key words: 1,3-ß-glucanase, Powdery mildew, Fungal inoculation, Gene expression, Wheat


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