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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (5): 861-866.doi: 10.3724/SP.J.1006.2009.00861

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Increasing Density of Wheat Genetic Linkage Map with Molecular Makers

LI Yan-Qiu1，SU Zhi-Fang12**，WANG Li-Xin1，JI Wei1，YAO Ji3，ZHAO Chang-Ping1*   

  1. 1Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing 100097,China;2Farming-grazing Science Department of Hetao University,Linhe 015000,China;3College of Life Science and technology,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2008-10-15 Revised:2008-12-31 Online:2009-05-12 Published:2009-03-23
  • Contact: ZHAO Chang-Ping E-mail:bjhwc2003@yahoo.com.cn

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Abstract:

High density genetic linkage map is the groundwork for mapping gene or quantitative trait loci, map-based cloning and marker-assisted selection. To increase the marker density on genetic linkage map of wheat (Triticum aestivum L.), the double haploid (DH) population derived from Jinghua 1/Xiaobai Dongmai and the recombinant inbred lines (RILs) of Nongda 015/Fuzhuang 30 were used in this study. A total of 339 polymorphic markers between the DH lines and 343 polymorphic markers between the RIL lines were detected. Using the DH population, 208 markers were mapped on 21 chromosomes, covering 3 493.6 cM; and using the RIL population, 299 markers were mapped on 34 linkage groups with the average distance of 15.5 cM. The two linkage maps had 56 consistent markers in the similar regions of chromosomes. Using Joinmap 4.0 software, ten linkage groups from the two linkage maps were integrated. This linkage map was composed of 217 markers and covered 956.2 cM of wheat genome with an average distance of 4.4 cM between markers. The proportion of segregation distortion loci was 3.2–55.6% on eight chroosomes. Most markers in this map had the consistent locations with those mentioned in previous report, however, five SSR markers were located on different chromosomes. The results enhance the density of wheat linkage map and provide more information for users.

Key words: Wheat, SSR, EST-SSR, SCAR, Linkage map


[1] Chao S, Sharp P J, Worland A J, Koebner R M D, Gale M D. RFLP-based genetic maps of homoeologous group 7 chromosomes. Theor Appl Genet, 1989, 78: 495–504

[2] Devos K M, Millan T, Gale M D. Comparative RFLP maps of homoeologous group 2 chromosomes of wheat, rye, and barley. Theor Appl Genet, 1993, 85: 784–792

[3] Devos K M, Gale M D. Comparative genetics in the grasses. Plant Mol Boil, 1997, 35: 3–15

[4] Williams J G K, Kubelik A R, Livak K J, Rafalski J A, Tingey S V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res, 1990, 18: 6531–6535

[5] Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M. AFLP: A new technique for DNA fingerprinting. Nucl Acid Res, 1995, 23: 4407–4414

[6] R?der M S, Korzun V, Wandehake K, Planschke J, Tixier M H, Leroy P, Gannal M W. A microsatellite map of wheat. Genetics, 1998, 149: 2007–2023

[7] Pestsova E, Ganal M W, R?der M S. Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome, 2000, 43: 689–697

[8] Gupta P, Balyan H, Edwards K, Isaac P, Korzun V, R?der M, Gautier M F, Joudrier P, Schlatter A, Dubcovsky J, De la Pena R, Khairallah M, Penner G, Hayden M, Sharp P, Keller B, Wang R, Hardouin J, Jack P, Leroy P. Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor Appl Genet, 2002, 105: 413–422

[9] Somers D J, Isaac P, Edwards K. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet, 2004, 109: 1105–1114

[10] Yu J K, Dake T M, Singh S, Benscher D, Li W, Gill B, Sorrells M E. Development and mapping of EST-derived simple sequence repeat markers for hexaploid wheat. Genome, 2004, 47: 805–818

[11] Gao L F, Jing R L, Huo N X, Li Y, Li X P, Zhou R H, Chang X P, Tang J F, Ma Z Y, Jia J Z. One hundred and one new microsatellite loci derived from ESTs (EST-SSRs) in bread wheat. Theor Appl Genet, 2004, 108: 1392–1400

[12] Chen H-M(陈海梅), Li L-Z(李林志), Wei X-Y(卫宪云), Li S-S(李斯深), Lei T-D(雷天东), Hu H-Z(胡海州), Wang H-G(王洪刚), Zhang X-S(张宪省). Exploitation, chromosomal location and genetic mapping of EST-SSRs in wheat. Chin Sci Bull (科学通报), 2005, 50(20): 2208–2216 (in Chinese)

[13] Levi A, Thomas C, Joobeur T, Zhang X, Davis. A genetic linkage map for watermelon derived from a testcross population: (Citrullus lanatus var. citroides × C. lanatus var. lanatus ) × Citrullus colocynthis. Theor Appl Genet, 2002, 105: 555–563

[14] Wu S B, Collins G, Sedgley M. A molecular linkage map of olive (Olea europaea L.) based on RAPD, microsatellite and SCAR markers. Genome, 2001, 47: 26–35

[15] Fischer B, Salakhutdinov I, Akkurt M, Eibach R, Edwards K, T?pfer R, Zyprian E. Quantitative trait locus analysis of fungal disease resistance factors on a molecular map of grapevine. Theor Appl Genet, 2004, 108: 501–515

[16] Ma J-C (马骥超), Chang N-T(常迺滔), Jiang J-L(姜俊龙), Li D-H(李大海),You X-Y(尤雪颜). Application of SSR marker in wheat resistant disease QTL and locating resistant gene. China Plant Prot (中国植保导刊), 2007, 27(6): 11–15(in Chinese with English abstract)

[17] Yang S-Z(杨随庄). Advance on study of molecular marker, gene location and gene engineering in drought resistance in wheat. Chin Agric Sci Bull (中国农学通报), 2007, 23(4): 59–63 (in Chinese with English abstract)

[18] Li W-C(李文才), Li T(李涛), Zhao F-T(赵逢涛), Li X-F(李兴锋), Wang H-G(王洪刚). QTL of wheat yield traits in D genome. Acta Agric Boreali-Sin (华北农学报), 2005, 20(1): 23–26 (in Chinese with English abstract)

[19] Li W-H(李卫华), Liu W(刘伟), Liu L(刘丽), Cao L-P(曹连莆). A review of qtl research for wheat quality traits. J Shihezi Univ (Nat Sci) (石河子大学学报·自然科学版), 2005, 23 (3): 389–394 (in Chinese with English abstract)

[20] Ji W(季伟), Wang L-X(王立新), Sun H(孙辉), Wang M-Y(王茅雁), Zhao C-P(赵昌平). Predigestion of wheat SSR analysis protocol. J Agric Biotechnol (农业生物技术学报), 2007, 15(5): 907–908 (in Chinese with English abstract)

[21] Li W-H(李卫华), Liu W(刘伟), You M-S(尤明山), Xu J(许杰), Liu C-L(刘春雷), Li B-Y(李保云), Liu G-T(刘广田). Construction of wheat molecular linkage map using different SSR markers and the polymorphism of the markers. J Triticeae Crops (麦类作物学报), 2007, 27(1): 1–6 (in Chinese with English abstract)

[22] Wang Z-L(王竹林), Liu S-D(刘曙东), Liu H-Y(刘惠远), He Z-H(何中虎), Xia X-C(夏先春), Chen X-M(陈新民). Acta Bot Boreal-Occident Sin (西北植物学报), 2006, 26(5): 886–892(in Chinese with English abstract)

[23] Nelson J C, Sorrells M E, Van-Deynze A E, Lu Y H, Atkinson M, Bernard M, Leroy P, Faris J D, Anderson J A. Molecular mapping of wheat: Major genes and rearrangements in homeologous 4, 5 and 7. Genetics, 1995, 141: 721–731

[24] Kojima T, Nagaoka T, Noda K. Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers. Theor Appl Genet, 1998, 96: 37–45

[25] Blanco A, Bellomo M P, Cenci A, De Giovanni C, D’Ovidio R, Iacono E, Laddomada B, Pagnotta M A, Porceddu E, Sciancalepore A, Simeone R, Tanzarella O. A genetic linkage map of durum wheat. Theor Appl Genet, 1998, 97: 721–728

[26] Song X-L(宋宪亮), Sun X-Z(孙学振), Zhang T-Z(张天真). Segregation distortion and its effect on genetic mapping in plants. J Agric Biotechnol (农业生物技术学报), 2006, 14(2): 286–292 (in Chinese with English abstract)
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