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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (11): 2064-2072.doi: 10.3724/SP.J.1006.2009.02064

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Proteome Changes in Wheat Varieties Brock and Jing 411 after Inoculating Blumenia graminis

YU Zhen1,LI Qian1,ZHAO Jian-Ye1,JIANG Fan1,WANG Zhen-Ying1,*,PENG Yong-Kang1*,XIE Chao-Jie2,LIU Zhi-Yong2,SUN Qi-Xin2,YANG Zuo-Min2   

  1. 1College of Chemistry and Life Science/Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation,Tianjin Normal University,Tianjin 300387,China;2College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China
  • Received:2009-02-12 Revised:2009-05-08 Online:2009-11-12 Published:2009-09-10
  • Contact: WANG Zhen-Ying, E-mail: wzycell@yahoo.com.cn;PENG Yong-Kang, E-mail: pykcell@yahoo.com.cn

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Abstract:

The protein of resistant wheat (Triticum aestivum L.) variety Brock and susceptible variety Jing 411 was extracted from leaves and separated using two-dimensional polyacrylamid gel electrophoresis (2-DE) at 12 h, 3 d, and 5 d after inoculating prevalent race No. 15 of B. graminis f.sp. tritici. In Brock, compared with contral with no pathogen inoculation, at least six protein spots of 43 kD/pI 6.7, 43 kD/pI 6.9, 43 kD/pI 7.2, 28 kD/pI 5.8, 26 kD/pI 5.5 and 26 kD/pI 6.5 obviously increased in content at 12 h time point; and five protein spots of 48 kD/pI 5.6, 43 kD/pI 6.9, 43 kD/pI 7.2, 28 kD/pI 5.8 and 26 kD/pI 5.5 increased in content at 3 d time point. At 5d after inoculation, 12 novel proteins were induced, viz. 16 kD/pI 7.6, 42 kD/pI 6.5, 40 kD/pI 4.8, 40 kD/pI 4.6, 31 kD/pI 5.7, 16 kD/pI 4.6, 20 kD/pI 8.3, 50 kD/pI 6.7, 48 kD/pI 6.6, 28 kD/pI 5.7, 23 kD/pI 4.8 and 25 kD/pI 4.7; simultaneously, two protein spots that were observed earlier disappeared. In Jing 411, three protein spots of 21 kD/pI 6.4, 18 kD/pI 5.4, and 14 kD/pI 7.0 increased in content at 12 h after inoculation. At the 3 d time point, two protein spots of 80 kD/pI 5.4 and 14 kD/pI 7.0 increased in content, however, one protein spot (16 kD/pI 5.4) showed decrease in protein abundance. At the 5 d time point, three protein spots of 50 kD/pI 7.3, 40 kD/pI 7.3, and 24 kD/pI 7.2 and two protein spots of 40 kD/pI 4.8 and 14 kD/pI 7.2 showed increase and decrease in protein abundance, respectively, but there were no novel protein spots induced. Among the 12 novel protein spots induced in Brock, six spots were identified using MALDI-TOF-MS and NCBI database searching, which were F-box and leucine-rich repeat protein, heavy metal transport/detoxification protein, endo-beta-1,3-glucanase (two isozymes), beta-1,3-glucanase precursor and zinc finger protein. These proteins are involved in a wide range of physiological processes, such as cell cycle control, development, phytohormone response, and resistance to fungal disease. Thus, the proteome changes in Brock and Jing 411 leaves are probably associated with the resistance and susceptibility to powder mildew, respectively.

Key words: Powdery mildew, Wheat, Proteome, Beta-1,3-glucanase, MALDI-TOF-MS


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