Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (11): 2064-2072.doi: 10.3724/SP.J.1006.2009.02064

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Proteome Changes in Wheat Varieties Brock and Jing 411 after Inoculating Blumenia graminis

YU Zhen1,LI Qian1,ZHAO Jian-Ye1,JIANG Fan1,WANG Zhen-Ying1,*,PENG Yong-Kang1*,XIE Chao-Jie2,LIU Zhi-Yong2,SUN Qi-Xin2,YANG Zuo-Min2   

  1. 1College of Chemistry and Life Science/Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation,Tianjin Normal University,Tianjin 300387,China;2College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China
  • Received:2009-02-12 Revised:2009-05-08 Online:2009-11-12 Published:2009-09-10
  • Contact: WANG Zhen-Ying, E-mail: wzycell@yahoo.com.cn;PENG Yong-Kang, E-mail: pykcell@yahoo.com.cn

PDF

1550

Cited

5

Abstract:

The protein of resistant wheat (Triticum aestivum L.) variety Brock and susceptible variety Jing 411 was extracted from leaves and separated using two-dimensional polyacrylamid gel electrophoresis (2-DE) at 12 h, 3 d, and 5 d after inoculating prevalent race No. 15 of B. graminis f.sp. tritici. In Brock, compared with contral with no pathogen inoculation, at least six protein spots of 43 kD/pI 6.7, 43 kD/pI 6.9, 43 kD/pI 7.2, 28 kD/pI 5.8, 26 kD/pI 5.5 and 26 kD/pI 6.5 obviously increased in content at 12 h time point; and five protein spots of 48 kD/pI 5.6, 43 kD/pI 6.9, 43 kD/pI 7.2, 28 kD/pI 5.8 and 26 kD/pI 5.5 increased in content at 3 d time point. At 5d after inoculation, 12 novel proteins were induced, viz. 16 kD/pI 7.6, 42 kD/pI 6.5, 40 kD/pI 4.8, 40 kD/pI 4.6, 31 kD/pI 5.7, 16 kD/pI 4.6, 20 kD/pI 8.3, 50 kD/pI 6.7, 48 kD/pI 6.6, 28 kD/pI 5.7, 23 kD/pI 4.8 and 25 kD/pI 4.7; simultaneously, two protein spots that were observed earlier disappeared. In Jing 411, three protein spots of 21 kD/pI 6.4, 18 kD/pI 5.4, and 14 kD/pI 7.0 increased in content at 12 h after inoculation. At the 3 d time point, two protein spots of 80 kD/pI 5.4 and 14 kD/pI 7.0 increased in content, however, one protein spot (16 kD/pI 5.4) showed decrease in protein abundance. At the 5 d time point, three protein spots of 50 kD/pI 7.3, 40 kD/pI 7.3, and 24 kD/pI 7.2 and two protein spots of 40 kD/pI 4.8 and 14 kD/pI 7.2 showed increase and decrease in protein abundance, respectively, but there were no novel protein spots induced. Among the 12 novel protein spots induced in Brock, six spots were identified using MALDI-TOF-MS and NCBI database searching, which were F-box and leucine-rich repeat protein, heavy metal transport/detoxification protein, endo-beta-1,3-glucanase (two isozymes), beta-1,3-glucanase precursor and zinc finger protein. These proteins are involved in a wide range of physiological processes, such as cell cycle control, development, phytohormone response, and resistance to fungal disease. Thus, the proteome changes in Brock and Jing 411 leaves are probably associated with the resistance and susceptibility to powder mildew, respectively.

Key words: Powdery mildew, Wheat, Proteome, Beta-1,3-glucanase, MALDI-TOF-MS


[1] Liu J-Y(刘金元), Tao W-J(陶文静), Duan X-Y(段霞瑜), Xiang Q-J(向齐君), Liu D-J(刘大钧), Chen P-D(陈佩度). Molecular marker assisted identification of Pm genes involved in the powdery mildew resistant wheat cultivars (lines). Acta Phytopathol Sin (植物病理学报), 2000, 30(2): 133-139 (in Chinese with English abstract)

[2] Liu J-Y(刘金元), Liu D-J(刘大钧). Progress of the study on wheat powdery mildew resistant genes. Acta Phytopathol Sin (植物病理学报), 2000, 30(4): 289-295 (in Chinese with English abstract)

[3] Yu L, Niu J S, Ma Z Q, Chen P D, Qi L L, Liu D J. Cloning, characterization and chromosome localization of two powdery mildew resistance-related gene sequences from wheat.Acta Bot Sin, 2002, 44: 1438-1444

[4] Yahiaoui N, Srichumpa P, Dudler R, Keller B. Genome analysis at different ploidy levels allows cloning of the powdery mildew resistance gene Pm3b from hexaploid wheat. Plant J, 2004, 37: 528-538

[5] Wang Z Y, Zheng Q, Peng Y K, Xie C J, Sun Q X, Yang Z M. Identification of random amplified polymorphism DNA and simple sequence repeat markers linked to powdery mildew resistance in common wheat cultivar Brock. Plant Prod Sci, 2004, 7: 319-323

[6] Wang Z Y, Zhao P, Chen H, Peng Y K, Xie C J, Sun Q X, Yang Z M. Random amplified polymorphic DNA and sequence characterized amplified region marker linked to unknown powdery mildew resistance gene in wheat cultivar Brock. Plant Prod Sci, 2005, 8: 578-585

[7] Wang Z-Y(王振英), Zhao H-M(赵红梅), Hong J-X(洪敬欣), Chen L-Y(陈丽媛), Zhu J(朱婕), Li G(李刚), Peng Y-K(彭永康), Xie C-J(解超杰), Liu Z-Y(刘志勇), Sun Q-X(孙其信), Yang Z-M(杨作民). Identification and analysis of four novel molecular markers linked to powdery mildew resistance gene Pm21 in 6VS chromosome short arm of Haynaldia villosa. Acta Agron Sin (作物学报), 2007, 33: 605-611 (in Chinese with English abstract)

[8] Zhang W-J(张维佳), Li C-Z(李纯正), Huang H-Q(黄海泉), Wang Z-Y(王振英), Peng Y-K(彭永康). Different proteins in mitochondrial proteome of T-tape maize cytoplasmic male-sterile line and its maintainer line. J Mol Cell Biol (分子细胞生物学报), 2007, 40(6): 410-418 (in Chinese with English abstract)

[9] Balmer Y, Vensel W H, DuPont F M, Buchanan B B, Hurkman W J. Proteome of amyloplasts isolated from developing wheat endosperm presents evidence of broad metabolic capability. J Exp Bot, 2006, 57: 1591-1602

[10] Zhao C F, Wang J Q, Cao M L, Zhao K, Shao J M, Lei T T, Yin J N, Hill G G, Xu N Z, Liu S Q. Proteomic changes in rice leaves during development of field-grown rice plants. Proteomics, 2005, 5: 961-972

[11] Cui S, Huang F, Wang J, Ma X, Cheng Y, Liu J. A proteomic analysis of cold stress responses in rice seedlings. Proteomics, 2005, 5: 3162-3172

[12] Yan S P, Tang Z C, Su W A, Sun W N. Proteomic analysis of salt stress-responsive proteins in rice root. Proteomics, 2005, 5: 235-244

[13] Salekdeh G H, Siopongco J, Wade L J, Ghareyazie B, Bennett J. Proteomic analysis of rice leaves during drought stress and recovery. Proteomics, 2002, 2: 1131-1145

[14] Frédérique R, Pascale G, Dominique V, Michel Z. Protein changes in response to progressive water deficit in maize. Plant Physiol, 1998, 117: 1253-1263

[15] Taylor N L, Heazlewood J L, Day D A, Millar A H. Differential impact of environmental stresses on the pea mitochondrial proteome. Mol Cell Proteomics, 2005, 4: 1122-1133

[16] Antonio J C, Christine C, Nathalie Z, Christian M, Emmanuelle L, Alain V D, Christophe C. Proteomic analysis of grapevine (Vitis vinifera L.) tissues subjected to herbicide stress. J Exp Bot, 2005, 56: 2783-2795

[17] Sun T Kim, Sang G K, Du H H, Sun Y K, Han J K, Byung H L, Jeung J L, Kyu Y K. Proteomic analysis of pathogen-responsive proteins from rice leaves induced by rice blast fungus Magnaporthe grisea.Proteomics, 2004, 4: 3569-3578

[18] Curto M, Camafeita E, Lopez J A, Maldonado A M, Rubiales D, Jorrín J V. A proteomic approach to study pea (Pisum sativum)responses to powdery mildew (Erysiphe pisi). Proteomics, 2006, 6: 163-174

[19] Wang Y, Yang L M, Xu H B, Li Q F, Ma Z Q, Chu C G. Differential proteomic analysis of proteins in wheat spikes induced by Fusarium graminearum. Proteomics, 2005, 5: 4496-4503

[20]Feng D-S(封德顺), Xu Q-Y(徐勤迎), Wang H-G(王洪刚), Tian J-C(田纪春). Changes of protein in wheat leaf after the infection of powdery mildew. Acta Agric Boreali-Sin (华北农学报)，2007, 22:123-126 (in Chinese with English abstract)

[21] Wang Z, Zhao P, Chen H, Peng Y, Xie C, Sun Q, Yang Z. Identification of RAPD markers and development of SCAR markers linked to a powdery mildew resistance gene, and their location on chromosome in wheat cultivar Brock. Plant Prod Sci, 2005, 8: 578-585

[22] Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anual Biochem,1976, 72: 248-254

[23] O’Farrell P H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem,1975, 250: 4007-4021

[24] Laemmli U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, 227: 680-685

[25] Neuhoff V, Arold N, Taube D, Ehrhardt W. Improved staining of proteins in polyacrylami de gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie brilliant blue G-250 and R-250. Electrophoresis, 1988, 9: 255-262

[26] Xie D X, Feys B F, James S, Nieto M, Turner J G.COI1: An Arabidopsis gene required for jasmonate regulateddefense and fertility. Science, 1998, 280: 1091-1094

[27] Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper J W, Elledge S J. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell, 1996, 86: 263-274

[28] Ward E R, Payne G B, Moyer M B. Differential regulation of β-1,3-glucanase messenger RNAs in response to pathogen infection. Plant Physiol, 1991, 96: 390-397

[29] Selitrennikoff C P. Antifungal proteins. Appl Environ Microbiol, 2001, 67: 2883-2894

[30] Anfoka G, Buchenauer H. Systemic acquired resistance in tomato against Phytophthora infestans by pre-inoculation with tobacco necrosis virus. Physiol Mol Plant Pathol, 1997, 50: 85-101

[31] Esquerré-Tugaé M T, Boudart G, Dumas B. Cell wall degrading enzymes, inhibitory proteins, and oligosaccharides participate in the molecular dialogue between plants and pathogens. Plant Physiol Biochem, 2000, 38: 157-163

[32] Klarzynski O, Plesse B, Joubert J M. Linear β-1,3-glucanase are elicitors of defense responses in tobacco. Plant Physiol, 2000, 124: 1027-1037

[33] Ham K S, Wu S C, Darvill A G. Fungal pathogens secrete an inhibitor protein that distinguishes isoforms of plant pathogenesis-related endo-β-1,3-glucanase. Plant J, 1997, 11: 169-179
[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] GUO Xing-Yu, LIU Peng-Zhao, WANG Rui, WANG Xiao-Li, LI Jun. Response of winter wheat yield, nitrogen use efficiency and soil nitrogen balance to rainfall types and nitrogen application rate in dryland [J]. Acta Agronomica Sinica, 2022, 48(5): 1262-1272.
[3] LEI Xin-Hui, WAN Chen-Xi, TAO Jin-Cai, LENG Jia-Jun, WU Yi-Xin, WANG Jia-Le, WANG Peng-Ke, YANG Qing-Hua, FENG Bai-Li, GAO Jin-Feng. Effects of soaking seeds with MT and EBR on germination and seedling growth in buckwheat under salt stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1210-1221.
[4] SHI Yu-Qin, SUN Meng-Dan, CHEN Fan, CHENG Hong-Tao, HU Xue-Zhi, FU Li, HU Qiong, MEI De-Sheng, LI Chao. Genome editing of BnMLO6 gene by CRISPR/Cas9 for the improvement of disease resistance in Brassica napus L [J]. Acta Agronomica Sinica, 2022, 48(4): 801-811.
[5] FU Mei-Yu, XIONG Hong-Chun, ZHOU Chun-Yun, GUO Hui-Jun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, ZHAO Shi-Rong, DING Yu-Ping, XU Yan-Hao, LIU Lu-Xiang. Genetic analysis of wheat dwarf mutant je0098 and molecular mapping of dwarfing gene [J]. Acta Agronomica Sinica, 2022, 48(3): 580-589.
[6] FENG Jian-Chao, XU Bei-Ming, JIANG Xue-Li, HU Hai-Zhou, MA Ying, WANG Chen-Yang, WANG Yong-Hua, MA Dong-Yun. Distribution of phenolic compounds and antioxidant activities in layered grinding wheat flour and the regulation effect of nitrogen fertilizer application [J]. Acta Agronomica Sinica, 2022, 48(3): 704-715.
[7] LIU Yun-Jing, ZHENG Fei-Na, ZHANG Xiu, CHU Jin-Peng, YU Hai-Tao, DAI Xing-Long, HE Ming-Rong. Effects of wide range sowing on grain yield, quality, and nitrogen use of strong gluten wheat [J]. Acta Agronomica Sinica, 2022, 48(3): 716-725.
[8] YAN Yan, ZHANG Yu-Shi, LIU Chu-Rong, REN Dan-Yang, LIU Hong-Run, LIU Xue-Qing, ZHANG Ming-Cai, LI Zhao-Hu. Variety matching and resource use efficiency of the winter wheat-summer maize “double late” cropping system [J]. Acta Agronomica Sinica, 2022, 48(2): 423-436.
[9] WANG Yang-Yang, HE Li, REN De-Chao, DUAN Jian-Zhao, HU Xin, LIU Wan-Dai, GU Tian-Cai, WANG Yong-Hua, FENG Wei. Evaluations of winter wheat late frost damage under different water based on principal component-cluster analysis [J]. Acta Agronomica Sinica, 2022, 48(2): 448-462.
[10] CHEN Xin-Yi, SONG Yu-Hang, ZHANG Meng-Han, LI Xiao-Yan, LI Hua, WANG Yue-Xia, QI Xue-Li. Effects of water deficit on physiology and biochemistry of seedlings of different wheat varieties and the alleviation effect of exogenous application of 5-aminolevulinic acid [J]. Acta Agronomica Sinica, 2022, 48(2): 478-487.
[11] XU Long-Long, YIN Wen, HU Fa-Long, FAN Hong, FAN Zhi-Long, ZHAO Cai, YU Ai-Zhong, CHAI Qiang. Effect of water and nitrogen reduction on main photosynthetic physiological parameters of film-mulched maize no-tillage rotation wheat [J]. Acta Agronomica Sinica, 2022, 48(2): 437-447.
[12] MA Bo-Wen, LI Qing, CAI Jian, ZHOU Qin, HUANG Mei, DAI Ting-Bo, WANG Xiao, JIANG Dong. Physiological mechanisms of pre-anthesis waterlogging priming on waterlogging stress tolerance under post-anthesis in wheat [J]. Acta Agronomica Sinica, 2022, 48(1): 151-164.
[13] MENG Ying, XING Lei-Lei, CAO Xiao-Hong, GUO Guang-Yan, CHAI Jian-Fang, BEI Cai-Li. Cloning of Ta4CL1 and its function in promoting plant growth and lignin deposition in transgenic Arabidopsis plants [J]. Acta Agronomica Sinica, 2022, 48(1): 63-75.
[14] WEI Yi-Hao, YU Mei-Qin, ZHANG Xiao-Jiao, WANG Lu-Lu, ZHANG Zhi-Yong, MA Xin-Ming, LI Hui-Qing, WANG Xiao-Chun. Alternative splicing analysis of wheat glutamine synthase genes [J]. Acta Agronomica Sinica, 2022, 48(1): 40-47.
[15] LI Ling-Hong, ZHANG Zhe, CHEN Yong-Ming, YOU Ming-Shan, NI Zhong-Fu, XING Jie-Wen. Transcriptome profiling of glossy1 mutant with glossy glume in common wheat (Triticum aestivum L.) [J]. Acta Agronomica Sinica, 2022, 48(1): 48-62.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd