理化诱变小豆京农6号突变体的鉴定

doi:10.3724/SP.J.1006.2010.00565

Abstract

Abstract:

Mutational approaches have been widely exploited in breeding, genetics and gene function researches. We firstly developed a large collection of mutants from adzuki bean (Vigna angularisi) cultivar Jingnong 6 treated by 0.5%, 0.9%, and 1.4% EMS for 12 h and 24 h, 400 Gy ⁶⁰Co-γ ray and 100 Gy, 300 Gy, and 600 Gy electron beam. The results indicated that the maximum mutantions were induced by 0.5% or 0.9% EMS for 24 h. More seed color mutants including cream-colored and light red seeds, pod color mutants such as brown, dark brown and black pods were produced by o.5% EMS. Needle leaf, sword, kidney-shaped and small heart-shaped leaf mutants were obtained by 0.9% EMS treatment. The percentage of mutants with 100, 300, and 600 Gy electron beam treatment were 4.09%, 3.64%, and 2.22% respectively. The percentage of mutants treated by 400 Gy ⁶⁰Co-γ ray was 7.23%. Nine hundred thirty-seven EMS-induced M₃ lines and nine hundred thirty-four M₂ lines radiated by ⁶⁰Co-γ ray and electron beam were generated. A total of 1 490 mutants were collected. Mutations included in plant height, plant architecture, leaf shape and color, leaf size, seed shape and color, seed size, pod color, branching type and number, bushy leaf or branch, sprawl, definite growth, sterile, early and late mature, flowering time. The mutant populations are very useful to genetic analysis of gene, gene mapping and cloning, and further the research on functional genomics. These mutants will be useful to serve the adzuki bean improvement.

Key words: Adzuki bean(Vigna angularisi), EMS and irradiation mutagenesis, Mutants, Mutant identification

TONG Xing,ZHAO Bo,JING Wen-Lin,ZENG Chao-Wu,LIU Hong-Xia,WU Bao-Mei,PU Shao-Jing. Identification of Mutants from Adzuki Bean (Vigna angularisi) Jingnong 6 seed Induced by Physical and Chemical Agents[J].Acta Agron Sin, 2010, 36(4): 565-573.

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Identification of Mutants from Adzuki Bean (Vigna angularisi) Jingnong 6 seed Induced by Physical and Chemical Agents

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