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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (05): 833-839.doi: 10.3724/SP.J.1006.2010.00833


Effect of Intracellular Calcium Stores on Hypersensitive Reaction Induced by Wheat Leaf Rust Fungus

ZHANG Bei,YAN Ai-Hua,LIU Gang,LIU Meng,HOU Chun-Yan,WANG Dong-Mei*   

  1. College of Life Science,Agricultural University of Hebei,Baoding 071001,China
  • Received:2009-11-09 Revised:2010-02-07 Online:2010-05-12 Published:2010-03-15
  • Contact: WANG Dong-Mei,E-mail:dongmeiwang63@hotmail.com E-mail:zjkzhangbei@sohu.com


With injecting drugs to affect intracellular calcium stores and calcium channel in Wheat (Triticum aestivum) leaves, we investigated hypersensitive reaction (HR) changes in wheat leaves invaded by leaf rust (Puccinia triticina). The results showed that the intracellular calcium chelators (BAPTA-AM) could obviously reduce the area of dying cells caused by HR. The higher the concentration was, the smaller the area of dying cell was. While intracellular calcium activator (caffeine) was injected into wheat leaves, HR could also be detected. The HR area increased with caffeine concentration. Calcium channel blockers (heparin, RR, and 8-Br-cADPR) were separately injected into wheat leaves before inoculation. The results showed that there was a concentration-depended effect on HR with herapin, and no obviously effect with RR and 8-Br-cADPR. Therefore, we deduced that intracellular calcium might involve in the formation of calcium signal transduction through IP3 pathway during infection of wheat leaf rust fungus.

Key words: Wheat(Triticum aestivum L.), Puccinia triticina, Calcium, Hypersensitive response

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