利用SPAD和Dualex快速、无损诊断玉米氮素营养状况

doi:10.3724/SP.J.1006.2010.00840

Abstract

Abstract:

Nitrogen (N) is one of the most important nutrients influencing both yield and grain quality, and N supply is one of the few production factors that can be controlled and which is known to effectively influence crop performance. Both SPAD reading and Dualex reading are shown to be highly correlated with leaf N concentration, and crop N status can be assessed through the SPAD reading and Dualex reading. The purpose of this study was to evaluate the suitable N rate at topdressing time. In order to evaluate the N application rates on SPAD reading and Dualex reading of corn after silking, corn field experiment was conducted with application of 45 kg N ha^-1 at sowing and planting density of 79 000 plants ha^-1 in 2008 on the L’Acadie experimental farm (Quebec, Canada). Topdressing N was performed with 0, 34, 68, 101, 135, 169 and 203 kg N ha^-1 at jointing stage. N-saturated reference plots with topdressing of 180 kg N ha^-1 at 10 days after sowing, besides 45 kg N ha^-1 at sowing, was established. With increasing topdressing N rates, SPAD reading, aboveground dry biomass and corn grain yield increased, and Dualex reading decreased. The SPAD-NSI of topdressing N treatments with 101, 135, 169 and 203 kg N ha^-1was always higher than 0.95 at all samplings dates. The Dualex-NSI of topdressing N treatments with 101 kg N ha^-1 was higher than 0.95 from 18 d to 46 d; the Dualex-NSI of topdressing N treatments with 135, 169 and 203 kg N ha^-1 was also higher than 0.95 at all samplings dates. SPAD reading, Dualex reading, SPAD-NSI and Dualex-NSI were strongly correlated with topdressing N rates. The maximum yield was reached when topdressing N was 101 or 135 kg N ha^-1at jointing stage. When maximum yield was reached, any additional N was no significant effect on SPAD reading, Dualex reading, aboveground dry biomass and corn grain yield. Topdressing N rate of 101 or 135 kg N ha^-1 was enough for corn growth after silking and getting maximum grain yield. There was no significant difference between splitting the N into two applications and N-saturated application on grain yield; however, splitting the N into two applications significantly decreased the total N application rate and growers’ inputs. In the present study, the suitable topdressing N rate was 101 or 135 kg N ha^-1with 45 kg N ha^-1at sowing. SPAD and Dualex could be integrated to evaluate in-season N status of corn.

Key words: Topdressing N application rates, Corn, SPAD reading, Dualex reading, Corn grain yield

YU Huan,WU Hua-Song,WANG Zhi-Jie. Evaluation of SPAD and Dualex for In-Season Corn Nitrogen Status Estimation[J].Acta Agron Sin, 2010, 36(05): 840-847.

References

[1] Elia A, Santamaria P, Serio F. Nitrogen nutrition, yield and quality of spinach. J Sci Food Agric, 1988,76: 341–346

[2] Samonte S O P B, Wilson L T, Medley J C, Pinson S R M, McClung A M, Lales J S. Nitrogen utilization efficiency: Relationships with grain yield, grain protein, and yield-related traits in rice. Agron J,2006, 98: 168–176

[3] Ferguson R B, Hergert G W, Schepers J S, Gotway C A, Cahoon J E, Peterson T A. Site-specific nitrogen management of irrigated maize: Yield and soil residual nitrate effects. Soil Sci Soc Am J,2002, 66: 544–553

[4] Arora Y, Juo A S R. Leaching of fertilizer ions in a Kaolinitic Ultisol in the high rainfall tropics: Leaching of nitrate in field plots under cropping and bare fallow. ,1982, 46: 1212–1218Soil Sci Soc Am J

[5] Randall G W, Vetsch J A, Huffman J R. Corn production on a subsurface-drained mollisol as affected by time of nitrogen application and nitrapyrin. Agron J, 2003, 95: 1213–1219

[6] Fox R H, Kern J M, Piekielek W P. Nitrogen fertilizer source, and method and time of application effects on no-till corn yields and nitrogen uptake. Agron J, 1986, 78: 741–746

[7] Russelle M P, Deibert E J, Hauck R D, Stevanovic M, Olson R A. Effects of water and nitrogen management on yield and ¹⁵N-depleted fertilizer use efficiency of irrigated corn. Soil Sci Soc Am J, 1981, 45: 553–558

[8] Welch L F, Mulvaney D L, Oldham M G, Boone L V, Pendleton J W. Corn yields with fall, spring, and sidedress nitrogen. Agron J, 1971, 63: 119–123

[9] Wood C W, Reeves D W, Duffield R R, Edmisten K L. Field chlorophyll measurements for evaluation of corn nitrogen status. J Plant Nutr,1992, 15: 487–500

[10] Arregui L M, Lasa B, Lafarga A, Iranneta I, Baroja E, Quemada M. Evaluation of chlorophyll meters as tools for N fertilization in winter wheat under humid Mediterranean conditions. Eur J Agron,2006, 24: 140–148

[11] Turner F T, Jund M F. Chlorophyll meter to predict nitrogen topdress requirement for semidwarf rice. Agron J, 1991, 83: 926–928

[12] Varvel G E, Schepers J S, Francis D D. Ability for in-season correction of nitrogen deficiency in corn using chlorophyll meters. Soil Sci Soc Am J,1997, 6: 1233–1239

[13] Schepers J S, Francis D D, Vigil M, Below F E. Comparison of corn leaf nitrogen concentration and chlorophyll meter readings. Commun Soil Sci Plan,1992,23: 2173–2187

[14] Hussain F, Bronson K F, Yadvinder S, Bijay S, Peng S. Use of chlorophyll meter sufficiency indices for nitrogen management of irrigated rice in Asia. Agron J, 2000, 92: 875–879

[15] Blackmer T M, Schepers J S. Use of a chlorophyll meter to monitor nitrogen status and schedule fertigation for corn. J Prod Agric,1995, 8: 56–60

[16] Tremblay N, Bélec C. Adapting nitrogen fertilization to unpredictable seasonal conditions with the least impact on-the environment. Horttechnology,2006, 16: 408–412

[17] Shapiro C A. Using a chlorophyll meter to manage nitrogen applications to corn with high nitrate irrigation water. Commun Soil Sci Plan, 1999, 30: 1037–1049

[18] Caldwell M M, Gold W G, Harris G, Ashurst C W. A modulated lamp system for solar UV-B (280–320 nm) supplementation studies in the field. Photochem Photobiol,1983, 37: 479–485

[19] Cerovic Z G, Ounis A, Cartelat A, Latouche G, Goulas Y, Meyer S, Moya I. The use of chlorophyll fluorescence excitation spectra for the non-destructive in situassessment of UV-absorbing compounds in leaves. Plant Cell Environ, 2002, 25: 1663–1676

[20] Cartelat A, Cerovic Z G, Goulas Y, Meyer S, Lelarge C, Prioul J L, Barbottin A, Jeuffroy M H, Gata P, Agati G, Moya I. Optically assessed contents of leaf polyphenolics and chlorophyll as indicators of nitrogen deficiency in wheat (Triticum aestivum L.). Field Crops Res,2005, 91: 35–49

[21] Goulas Y, Cerovic Z G, Cartelat A, Moya I. Dualex: a new instrument for field measurements of epidermal UV-absorbance by chlorophyll fluorescence. Appl Optics, 2004, 43: 4488–4496

[22] Tremblay N, Wang Z J, Bélec C. Evaluation of the Dualex for the assessment of corn nitrogen status. J Plant Nutr, 2007, 30: 1355–1369

[23] Delgado J A, Ristau R J, Dillon M A, Duke H R, Stuebe A, Follett R F, Shaffer M J, Riggenbach R R, Sparks R T, Thompson A, Kawanabe L M, Kunugi A, Thompson K. Use of innovative tools to increase nitrogen use efficiency and protect environmental quality in crop rotations. Commun Soil Sci Plan, 2001, 32: 1321–1354

[24] Ma B L, Dwyer L M, Gregorich E G. Soil N amendment, effects on seasonal N mineralization and N cycling in maize production. Agron J, 1999, 91: 1003–1009

Jia L-L(贾良良), Chen X-P(陈新平), Zhang F-S(张福锁). Non-destructive measurement of crop nitrogen nutrition diagnosis. World Agric (世界农业), 2001, (6): 36–37 (in Chinese with English abstract)

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Evaluation of SPAD and Dualex for In-Season Corn Nitrogen Status Estimation

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