不同品质类型玉米籽粒充实期的胚乳细胞增殖与生理活性比较

doi:10.3724/SP.J.1006.2010.00848

Abstract

Abstract:

The multiplication of endosperm cell and the physiological characteristics in developing kernels were investigated among normal corn, glutinouscorn and pop corn in this study. The number of endosperm cell, the traits of carbohydrate accumulation, the change of hormone content and the activities of enzymes related to starch corn, the process of kernel endosperm cell proliferation was earlier than that of the dry matter increase; the number of kernel endosperm cells reached the maximum at 24, 18, 13 days after pollination, while kernel weight at 45, 40, 30 d respectively. In the process of starch accumulation, the total content of starch of glutinous corn was higher than that of normal corn and pop corn at the same period, especially at 30 d after pollination and the difference was obvious, and that between normal corn and pop corn was not distinct; the difference of the speed of kernel starch accumulation among different types was mainly manifested in two aspects faster or slower and earlier or later for accumulation. The maximum of glutinous corn starch accumulation rate was 1.61, 1.34 times higher than that of normal corn and pop corn. The maximum rate of starch accumulation of glutinous corn appeared at 10 d after pollination, while that of normal corn and pop corn at 20 d after pollination. The number of endosperm cell was significantly correlated with the filling rate of kernel in normal corn at the 0.01 probability level, and in glutinous corn at 0.05 level, while not significantly correlated in pop corn. In kernel-filling period, the content of ZR was higher in normal corn than in glutinous corn and pop corn at the same period, moreover, in the glutinous corn than in pop corn. The differences among the three typesin the content of IAA, ABA, and GA₃ were exactly opposite to those of ZR_.The difference of SS activity among the three quality types was not significant, but in later kernel-filling stage, the SS activity declined slowly in normal corn and fast in pop corn fast. The average activity of SSS and synthesis were compared in different types of corn. The results showed that for normal, glutinous and popADPG-PPase of glutinous corn was significantly higher than that of normal corn and pop corn in the kernel-filling period. The average activity of GBSS of pop corn was higher than that of normal corn and glutinous corn. The activity of GBSS for pop corn kernel was the highest one in the three corn types.

Key words: Corn, Quality type, Kernel, Endosperm cell, Hormone, Enzyme activities

ZUO Zhen-Peng, WANG Jing, DONG Lu-Hao, MA Deng-Chao, SUN Qing-Quan, DONG Shu-Ting. Comparison of Multiplication of Endosperm Cell and Physiological Activity in Developing Kernels among Normal Corn, Glutinous Corn and Pop Corn[J].Acta Agronomica Sinica, 2010, 36(05): 848-855.

References

[1]Sun Q-Q(孙庆泉), Hu C-H(胡昌浩), Dong S-T(董树亭), Wang K-J(王空军). Advances of physiological characters studies about leaf source and kernel sink on cultivars in maize. J Shandong Agric Univ (山东农业大学学报), 1999, 30(4): 484-492 (in Chinese with English abstract)
[2]Jia S-F(贾士芳), Dong S-T(董树亭), Wang K-J(王空军), Zhang J-W(张吉旺), Li C-F(李从锋). Effect of shading on kernel quality at different stages from flowering to maturity in maize. Acta Agron Sin (作物学报), 2007, 33(12): 1960-1967 (in Chinese with English abstract)
[3]Sun Q-Q(孙庆泉), Wu Y-Q(吴元奇), Hu C-H(胡昌浩), Dong S-T(董树亭), Rong T-Z(荣廷昭), Zhang Y(张颖). Physiological activities and multiplication of endosperm cell at filling stage of kernels with different yield potential in maize. Acta Agron Sin (作物学报), 2005, 31(5): 612-618 (in Chinese with English abstract)
[4]Li S-C(李绍长), Lu J-H(陆嘉惠), Meng B-M(孟宝民). The relationship between endosperm cell differentiating and kernel filling. J Maize Sci (玉米科学), 2000, 8(4): 45-47 (in Chinese with English abstract)
[5]Wang X-Y(王晓燕), Dong S-T(董树亭), Gao R-Q(高荣岐), Zhang H-Y(张海艳). Endosperm cell proliferating and its relation to kernel weight in different types of maize. Acta Agric Boreal-Sin (华北农学报), 2006, 21(2): 23-26 (in Chinese with English abstract)
[6]Zhang H Y, Dong S T, Gao R Q, Sun Q Q. Starch accumulation and enzyme activities associated with starch synthesis in maize kernels. Agric Sci China, 2007, 6(7): 808-815
[7]Pinto. Influence of endosperm cell number on kernel size and weight in maize. Dissertation Abstracts International. B. Science and Engineering. 1986, 46(11): 3652-3653
[8]Liu P(刘鹏), Hu C-H(胡昌浩), Dong S-T(董树亭), Wang K-J(王空军), Zhang J-W(张吉旺), Zhang B-R(张保仁). Comparison of enzymes activity associated with sucrose metabolism in the developing kernels between sweet corn and normal corn. Sci Agric Sin (中国农业科学), 2005, 38(1): 52-58 (in Chinese with English abstract)
[9]Zuo Z-P(左振朋), Sun Q-Q(孙庆泉), Dong L-H(董鲁浩), Wang J(王婧), Ma D-C(马登超), Dong S-T(董树亭). Comparison of photosynthetic characteristics of leaves in pop corn, sweet corn and glutinous corn during the late growing period
[J]. Acta Agron Sin (作物学报.2009, 35(10):1930-1935

[10]Wang W-H(王蔚华), Guo W-S(郭文善), Fang M-K(方明奎). Endosperm cell proliferating and kernel filling dynamics in wheat. Acta Agron Sin (作物学报), 2003, 29(5): 779-784 (in Chinese with English abstract)
[11]Song J-M(宋健民), Dai S(戴双), Li H-S(李豪圣), Liu A-F(刘爱峰), Cheng D-G(程敦公), Chu X-S(楚秀生), Ian J T, Michael J E. Expression of a wheat endosperm 14-3-3 protein and its interactions with starch biosynthetic enzymes in amyloplasts
[J]. Acta Agron Sin (作物学报.2009, 35(8):1445-1450

[12]Yang J-C(杨建昌), Wang G-Z(王国忠), Wang Z-Q(王志琴), Liu L-J(刘立军), Zhu Q-S(朱庆森). Kernel-filling characteristics and changes of hormonal content in the kernels of dry-cultivated rice during kernel-filling. Acta Agron Sin (作物学报), 2002, 28(5): 615-621 (in Chinese with English abstract)
[13]Yang J-C(杨建昌), Wang Z-Q(王志琴), Zhu Q-S(朱庆森). Regulation of abscisic acid to the kernel filling of indica-japanica hybrid rice. J Jiangsu Agric Coll (江苏农学院学报), 1995, 16(4): 126-129 (in Chinese with English abstract)
[14]Yang J-C(杨建昌), Qiu M(仇明), Wang Z-Q(王志琴), Liu L-J(刘立军), Zhu Q-S(朱庆森). The relation between endosperm cell division and ZR content of kernel in rice. Acta Agron Sin (作物学报), 2004, 30(31): 11-17 (in Chinese with English abstract)
[15]Zhong L-J(钟连进), Cheng F-M (程方民). Varietal differences in amylose accumulation and activities of major enzymes associated with starch synthesis during grain filling in rice. Acta Agron Sin (作物学报), 2003, 29(3): 452-456 (in Chinese with English abstract)
[16]Cheng F-M(程方民), Jiang D-A(蒋德安), Wu P(吴平), Shi C-H(石春海). The dynamic change of starch synthesis enzymes during the kernel filling stage and effects of temperature upon it. Acta Agron Sin (作物学报), 2001, 27(2): 201-206 (in Chinese with English abstract)
[17]Douglas C D, Tsung M K, Frederick C F. Enzymes of sucrose and hexose metabolism in developing kernels of two inbreds of maize
[J].Plant Physiol
[18]Nakamura Y, Yuki K, Park S Y. Carbohydrate metabolism in the developing endosperm of rice kernels. Plant Cell Physiol, 1989, 30: 833-839
[19]Wu S-R(吴颂如), Chen W-F(陈婉芬), Zhou X(周燮). Enzyme linked immunosorbent assay for endogenous plant hormones. Plant Physiol Commun (植物生理学通讯), 1988, (5): 53-57 (in Chinese)
[20]Li B-H(李伯航), Cui Y-H(崔彦宏). Studies on the relationship between endosperm cell development and kernel weight of summer-maize (Zea mays L.). J Hebei Agric Coll (河北农业大学学报), 1989, 12(4): 39-45 (in Chinese with English abstract)
[21]Reddy V M. Endosperm characteristics associated with rate of kernel filling and kernel size in corn. Madica, 1983, 38: 339-355
[22]Zhang J-J, Hu Y-F, Zhou H, Huang Y-B. Starch accumulation and activities of key enzymes involved in starch synthesis in the kernels of maize inbred lines with different starch contents. J Plant Physiol Mol Biol,2007, 33: 123-130
[23]Zhang H Y, Dong S T, Gao R Q, Li Y Q. Comparison of starch synthesis and related enzyme activities in developing kernels among different types of maize. J Plant Physiol Mol Biol, 2007, 33: 25-32
[24]Tsai C Y, Nelson O E. Starch-deficient maize mutant lacking adenosine diphosphate glucose pyrophosphorylase activity. Science, 1966, 151: 341-343
[25]Nelson O E, Pan D. Starch synthesis in maize endosperms. Annu Rev Plant Physiol Plant Mol Biol, 1995, 46: 475-496
[26]Zhang J-W(张吉旺), Dong S-T(董树亭), Wang K-J(王空军), Hu C-H(胡昌浩), Liu P(刘鹏). 作物学报), 2008, 34(8): 1470-1474 (in Chinese with English abstract)Effects of shading in field on key enzymes involved in starch synthesis of summer maize. Acta Agron Sin (
[27]Blauth S L, Kim K N, Klucinec J, Shannon J C, Thompson D, Guiltinan M. Identification of mutator insertional mutants of starch branching enzyme1 (sbe1) in Zea mays L
[J].Plant Mol Biol
[28]Wang R-Y(王瑞英), Yu Z-W(于振文), Pan Q-M(潘庆民), Xu Y-M(许玉敏). Changes of endogenous plant hormone contents during kernel development in wheat. Acta Agron Sin (作物学报), 1999, 25(2): 225-231 (in Chinese with English abstract)
[29]Borras L, Cura J A, Otegui M E. Maize kernel composition and post-flowering source-sink ratio
[J].Crop Sci
[30]Yang J-C(杨建昌), Liu K(刘凯), Zhang S-F(张慎凤), Wang X-M(王学明), Wang Z-Q(王志琴), Liu L-J (刘立军). Hormones in rice spikelets in responses to water stress during meiosis
[J].Acta Agron Sin (作物学报.2008, 34(1):111-118
[31]Tao L-X(陶龙兴), Wang X(王熹), Huang X-L(黄效林). Effects of endogenous IAA on kernel filling of hybrid rice. Chin J Rice Sci (中国水稻科学), 2003, 17(2): 149-155 (in Chinese with English abstract)
[32]Rook F, Corker F, Card R. Impaired sucrose-induction mutants reveal the modulation of sugar-induced starch biosynthetic gene expression by abscisic acid signaling
[J].Plant J

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Comparison of Multiplication of Endosperm Cell and Physiological Activity in Developing Kernels among Normal Corn, Glutinous Corn and Pop Corn

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