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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (06): 968-978.doi: 10.3724/SP.J.1006.2010.00968

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Differentially Expressed Genes in Oleic Acid Synthesis of Brassica napus by Gene Chip

GUAN Mei, LI Xun, GUAN Chun-Yun   

  1. Oil Crops Research Institute,Hunan Agricultural University,Changshe 410128,China
  • Received:2009-10-10 Revised:2010-03-19 Online:2010-06-12 Published:2010-04-14
  • Contact: LI Xun,E-mail: lixun392007@163.com; Tel: 0731-84618782

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Abstract:

The oil from rapeseed with high oleic oil content is insensitive to oxidation and longer shelf life. In daily diet high oleic acid oil can reduce low-density lipoprotein cholesterol levels in blood and prevent arteriosclerosis. In addition, high oleic acid content in edible oil is an important fat indicator. Oleic acid can be effectively converted to methyl ester and is beneficial to produce biodiesel. Oleic acid content in conventional rapeseed is only 17%. However, oleic acid content in low erucic acid rapeseed reaches around 60%. Differential expressed genes of oleic acid have been found in thehigh oleic acid line (71.71%) and low oleic acid line (55.6%) of Brassica napus by gene chip technic. The results showed that there were 562 differential expressed genes relating to high and low oleic acid contents in rapeseed, among them 194 were up-regulated genes and 368 were down-regulated genes. The up-regulated gene (NM_100489) and the down-regulated gene (NM_130183) were then taken as the materials. Real time RT-PCR was further used to confirm the results of gene chip, and the results of real time RT-PCR analysis agreed with the results by gene chip. According to the results of gene chip, 562 differentially expressed genes were annotated functionally by Go Annotation System. The molecular function of differential expressed genes mainly included catalysis of enzymes, binding, transcriptional regulation, metabolism and others which were unknown. Some genes relating to sugar metabolism and fatty acid synthesis were identified as differential expressed genes. They are pyruvate kinase, fructose diphosphate, acyl transfer/acyl-ACP S lipase, hydrolase acting on ester bond, D9 stearoyl acid-N-carrier protein desaturase (ADS1), D9 acyl-lipid desaturase 2 (ADS2), ω-3 fatty acid desaturation enzymes (fad3) and so on.

Key words: Gene chip, Rapeseed, Oleic acid, Differential expressed genes

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