Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (11): 1877-1882.doi: 10.3724/SP.J.1006.2010.01877

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Effects of Anti-Sense Thioredoxin s on Grain Yield and Quality Properties in Two Wheat Cultivars with Different Quality Types

REN  Jiang-Ping,WANG  Na,WANG  Xin-Guo,LI  Yong-Chun,NIU  Hong-Bin,WANG  Xiang,YIN  Jun   

  1. Henan Agricultural University/National Engineering Research Centre for Wheat,Zhengzhou 450002,China
  • Received:2010-03-16 Revised:2010-05-29 Online:2010-11-12 Published:2010-08-04
  • Contact: Yin Jun,E-mail:xmzxyj@126.com;Tel:0371-63558203

PDF

1806

Cited

3

Abstract: Thioredoxin h (Trxh) is a small protein catalyzing thiol-disulfide interchange, which is involved in many processes, such as activation or deactivation of enzymes and enzyme inhibition and germination. Wheat pre-harvest sprouting is caused by a series of hydrolyses, in which α-amylase plays a key role. The anti-trxs gene has been transferred into wheat (Triticum aestivum L.) cultivars “Yumai 18” and “Yumai 34” since 2003 to decrease Trxhreductive capability and α-amylase activity in wheat seed. From the T4 generations, two transgenic lines, TY18 and TY34 were obtained. To investigate the effects of anti-sense thioredoxin s on grain yield and quality properties, TY18 and TY34 were tested in field experiments in 2007–2009 growing seasons with comparisons to their cultivars Yumai 18 (weak gluten) and Yumai 34 (strong gluten ). The result showed that the grain yield and quality properties of both lines were greatly affected. Compared with the wild controls, grain number per spike, grain yield and the starch quality in seeds of the transgenic lines were significantly promoted. The total starch content, amylopectin content, peak viscosity, trough viscosity, and final viscosity were significantly increased in transgenic lines, and the ratio amylase-to-amylopectin reduced, accordingly. The effects of transferring gene on protein quality were different in the two cultivars. The contents of total protein, albumin, globulin, and glutelin decreased significantly and the contents of gliadin increased significantly in the two transgenic lines of TY18. The development time and stability time of flour decreased significantly. However, these protein quality indexes had no significant differences between transgenic lines and controls, except contents of albumin, gliadin, and glutelin, which were decreased in transgenic lines TY34. Therefore, the anti-trxs gene transferred into wheat improved the starch quality of both wheat cultivars and the braking quality of weak-gluten cultivar.

Key words: Wheat, Anti-sense thioredoxin s gene, Yield, Processing quality

[1]Xiao S-H(肖世和), Yan C-S(闫长生), Zhang H-P(张海萍), Sun G-Z(孙果忠). Study on Wheat Pre-harvest Sprouting (小麦穗发芽研究). Beijing: China Agricultural Science and Technology Press, 2004. pp 199–218 (in Chinese)
[2]Jia J-Z(贾继增), Zhang Z-B(张正斌), Devos K, Gale M D. RFLP mapping of wheat chromosome 21 locus analysis of genetic di-    versity. Sci China (Ser C)(中国科学×C辑), 2001, 31(1): 13–21 (in Chinese)
[3]Hou W-S(侯文胜), Guo S-D(郭三堆), Lu M(路明). Gene transformation for plant improvement. Biotechnol Inf (生物技术通报), 2002, (1): 10–15 (in Chinese with English abstract)
[4]Joudrier P, Gautier M, Lamotte F D, Kobrehel K. The thioredoxin h system: potential applications. Biotechnol Adv, 2005, 23: 81– 85
[5]Kobrehel K, Yee B C, Buchanan B B. Role of the NADP/thioredoxin system in reduction of α-amylase and trypsin inhibitor proteins. J Biol Chem, 1991, 266: 16135–16140
[6]Kobrehel K, Wong J H, Balogh A, Kiss F, Yee B C, Buchanan B B. Specific reduction of wheat storage proteins by thioredoxin h. Plant Physiol, 1992, 99: 919–924
[7]Ballicora M A, Frueauf J B, Fu Y, Schurmann P, Preiss J. Activation of the potato tuber ADP-glucose pyrophosphatase by thioredoxins. J Biol Chem, 1999, 275: 1315–1320
[8]Wong J H, Kobrehel K, Nimbona C, Yee B C, Balogh A, Kiss F. Thioredoxin and bread wheat proteins. Cereal Chem, 1993, 70: 113–114
[9]Li X M, Nield J, Hayman D, Langredge P. Cloning a putative self-incompatibility gene from the pollen of the grass Phalaris coerulescens. Plant Cell, 1994, 6: 1923–1932
[10]Li X M, Nield J, Hayman D, Langridge P. A self-fertile mutant of Phalaris produces an S protein with reduced thioredoxin activity. Plant J, 1996, 10: 505–513
[11]Yin J(尹钧), Ren J-P(任江萍), Song L(宋丽), Li L(李磊), Shi X-Z(师学珍), Guo X-Y(郭向云). Regeneration culture from bombarded immature embryos of wheat and transgenic plant. Acta Bot Boreal-Occident Sin (西北植物学报), 2003, 23(9): 1565–1570 (in Chinese with English abstract)
[12]Liu L(刘雷), Yin J(尹钧), Ren J-P(任江萍), Han J-F(韩锦峰). Effects of antisense trxs on germination of transgenic wheat seeds. Acta Agron Sin (作物学报), 2004, 30(8): 801–805 (in Chinese with English abstract)
[13]Ren J-P(任江萍), Guo J-J(郭建军), Wang X-G(王新国), Li L(李磊), Yin J(尹钧). Inheriting analysis of anti-trxs gene and pre-harvest sprouting resistant characteristic in transgenic line 01TY18. Acta Bot Boreal-Occident Sin (西北植物学报), 2008, 28(6): 941–945 (in Chinese with English abstract)
[14]He Z-F(何照范). Analysis Techniques for Grain Quality in Ce- reals and Oils (粮油籽粒品质及其分析技术). Beijing: China  Agriculture Press 1985. pp 131–133 (in Chinese)
[15]Serrato A J, Cejudo F J. Type-h thioredoxins accumulate in the nucleus of developing wheat seed tissues suffering oxidative stress. Planta, 2003, 217: 392–399
[16]Zhang C-Q(张春庆), Li Q-Q(李晴祺). A study on the main quality characters influencing the steamed bread processing quality of T. aestivum. Sci Agric Sin (中国农业科学), 1993, 26(2): 39–46 (in Chinese with English abstract)
[17]Yao D-N(姚大年), Li B-Y(李保云), Zhu J-B(朱金宝). Study on main starch properties an d predictive indexes of noodle quality in common wheat (Triticum aestivum). Sci Agric Sin (中国农业科学), 1999, 32(6): 84-88 (in Chinese with English abstract)
[18]Zhang G-Q(张国权), Wei Y-M(魏益民), Ou-Yang S-H(欧阳韶辉), Xi M-L(席美丽), Hu X-Z(胡新中), Sietz W. Noodle quality characters of wheat varieties. J Chin Cereals Oils Assoc (中国粮油学报), 2000, 15(3): 5–7 (in Chinese with English abstract)
[19]Zeng M, Morris C F, Batey I L, Wrigley C W, Zeng M. Sources of variation for starch gelatinization, pasting, and gelation properties in wheat. Cereal Chem, 1997, 74: 63–71
[20]Guo H-X(郭红祥), Yin J(尹钧), Ren J-P(任江萍), Wang Z-Y(王振云). Effects of antisense trxs on starch hydrolases of wheat (Triticum aestivum L.) seeds in germination. Plant Physiol Commun (植物生理学通讯), 2007, 43(2): 273–276 (in Chinese with English abstract)
[21]Li Z-X(李志西), Wei Y-M(魏益民), Zhang J-G(张建国), Zhang G-Q(张国权). Study on the effects of wheat protein components on dough properties and baking quality. J Chin Cereals Oils Assoc (中国粮油学报), 1998, 13(3): 1–5 (in Chinese with English abstract)
[22]Ma C-X(马传喜), Wu Z-S(吴兆苏). Effect of variation of protein fractions and HMW glutenin subunits on SDS sedimentation volume in wheat varieties. Acta Agron Sin (作物学报), 1993, 19(6): 562–567 (in Chinese with English abstract)
[23]Xu Z-F(徐兆飞), Zhang H-Y(张惠叶), Zhang D-Y(张定一). Wheat Quality and Improvement (小麦品质及其改良). Beijing: Meteorological Press, 1999. pp 22–26 (in Chinese)
[24]Li Y-Q(李永强), Zhai H-M(翟红梅), Tian J-C(田纪春). Effect of protein and starch contents on wheat dough rheological properties. Acta Agron Sin (作物学报), 2007, 33(6): 937–941 (in Chinese with English abstract)
[25]Grasp W, Carpenter H C, Anderssen R S. Modelling the developmental rheology of wheat flour dough using extension tests. J Cereal Sci, 2000, 31: 1–13
[1] Zhai Sheng-Nan, Cao Xin-You, Li Hao-Sheng, Li Ji-Hu, Li Fa-Ji, Liu Jin-Dong, Xia Xian-Chun, Lyu Ying-Ying, Ma Rui-Feng, Wang Ying, Geng Hong-Wei, Liu Jian-Jun. Analysis of the genetic effects of allelic variation at the Pod-A1, Pod-D1, and Pod-2D loci on peroxidase activity in wheat grains [J]. Acta Agronomica Sinica, 2026, 52(6): 1593-1603.
[2] Xi Qian-Hui, Xu Zi-Yuan, Liu Meng-Meng, Wang Hong-Yi, Lang Kai-Lin, Jing Zhen-Hai, Chen Feng, Zhao Lei. Genome-wide association study and candidate gene prediction of grain copper content in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1604-1617.
[3] Mao Jia-Qi, Huang Peng-Yu, Zhao Jia-Jia, Zheng Xing-Wei, Wu Bang-Bang, Hao Yu-Qiong, Qu Fei, Liu Cheng, Ma Peng-Tao, Zheng Jun. Evaluation of powdery mildew resistance in wheat cultivars and molecular detection of resistance genes in Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(6): 1669-1681.
[4] Hu Chuan, Zhao Kai-Nan, Huang Xiu-Li, Wu Jin-Zhi, Ren Kai-Ming, Wang He-Zheng, Fu Guo-Zhan, Huang Ming, Li You-Jun. Effects of tillage methods and nitrogen rates on yield and quality of dryland wheat under one-off irrigation [J]. Acta Agronomica Sinica, 2026, 52(6): 1830-1846.
[5] Chen Xue-Yan, He Hua-Chuan, Li Zheng-Jia, Dong Xin-Pan, Li Ou-Qi, Liu Xiao-Yun, Li Dan-Ping, Chen Zhi-Wei, Liu Guo-Xia, Lyu Sheng-Yuan, Wu Yin-Ying, Zhao Zhen-Dong, Cao Xin-You, Wan He-Ping. Dynamic changes in root organic acid secretion and its transcriptional regulatory mechanisms in ‘Jimai 60’ seedlings under combined salinity-alkalinity stress in hydroponics [J]. Acta Agronomica Sinica, 2026, 52(6): 1859-1875.
[6] Gao Pei-Yang, Li Jin-Xuan, Dong Yu-Kui, Shi Yu, Zhang Zhen, Zhang Yong-Li. Response of wheat tillering and spike formation to nitrogen rate under supplementary irrigation based on soil moisture content [J]. Acta Agronomica Sinica, 2026, 52(6): 1847-1858.
[7] Ma Sheng-Qian, Wang Zhi-Ping, Chen Hao-Tian, Dou Shu-Xian, Zhang Yan, Deng Ai-Xing, Zhang Wei-Jian, Yuan Xiang-Yang, Song Zhen-Wei. Effects of tillage methods and nitrogen application rate on maize yield and soil aggregates in northeastern China under straw returning [J]. Acta Agronomica Sinica, 2026, 52(6): 1802-1816.
[8] Zhang Xian-Feng, Guo Li-Jian, Li Kang-Chun, Kong Bin-Xue, Liu Yu-Fang, Che Zhuo, Yang De-Long. Identification of the ABHD6 gene family and development of functional markers for grain weight in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1711-1727.
[9] Zhang Ning-Ning, Teng Yu-Fei, Ren Na-Na, Wei Xing-Zhuo, Yan Shu-Hao, Fan Ke-Xin, Wang Yong-Hong, Chen Wen-Kang, Zhang Xing-Hua, Zhu Wan-Chao, Xu Shu-Tu, Xue Ji-Quan. Phenotypic evaluation and plasticity analysis of drought resistance in 201 maize inbred lines [J]. Acta Agronomica Sinica, 2026, 52(5): 1309-1325.
[10] Zhang Si-Si, Zhao Xiang-Hui, Zhou Yang, Yao Yun-Feng, Zhu Rong-Yu, Dong Yuan-Jie, Hu Guo-Qing, Xu Tong, Liu Zhao-Xin. Effects of plowing and green manure returning in winter fallow period on soil physicochemical properties and yield in continuously cropped peanut [J]. Acta Agronomica Sinica, 2026, 52(5): 1472-1486.
[11] Wang Zhuang-Zhuang, Wu Zi-Jun, Zhang Yong-Xin, Zhang Xin-Yuan, Yuan Li-Xue, Chen Ru-Xue, Liu Shi-Ju, Duan Jian-Zhao, Feng Wei, Wang Tong-Chao, Wang Yong-Hua. Optimized water-nitrogen synergy enhances winter wheat yield and nitrogen use efficiency in clay-loam fluvo-aquic soils of southeastern Henan, China [J]. Acta Agronomica Sinica, 2026, 52(5): 1501-1521.
[12] Liu Xin-Meng, Ren Hao, Zhang Ji-Bo, Zhang Ji-Wang, Zhao Bin, Ren Bai-Zhao, Liu Peng, Wang Hong-Zhang. Physiological mechanisms of methyl jasmonate (MeJA) alleviating the effects of heat stress on ear differentiation in maize [J]. Acta Agronomica Sinica, 2026, 52(5): 1561-1572.
[13] Zhang Hong-Rong, Wang Fei-Er, Li Pan, Qiu Hai-Long, Zhu Jing, Zhao Lian-Hao, Nan Yun-You, He Wei, Fan Zhi-Long, Hu Fa-Long, Chai Qiang, Yin Wen. Photosynthetic characteristics of 20% reduced irrigation combined with 25% organic substitution for chemical fertilizer in increasing silage maize yield [J]. Acta Agronomica Sinica, 2026, 52(5): 1487-1500.
[14] Zhao Jia-Xue, Zhou Long-Hao, Guo Qi-Yuan, Shang Lun-Xiao, Wang Han, Liu Zhi-Tao, Chen Xi, Zhang Xiao-Pei, Song Xian-Liang, Ahmedov Miraziz Baltaevich, Mao Li-Li. Long-term stubble return and subsoiling enhance cotton yields in coastal saline-alkali soils by improving soil conditions and photosynthetic characteristics [J]. Acta Agronomica Sinica, 2026, 52(5): 1548-1560.
[15] Guo Xing-Yu, Hu Dan, Lin Su-Qi, Wang Meng-Kai, Tan Wen-Feng, Huang Chuan-Qin. Biochar combined with chemical fertilizer increases maize yield and soil ecosystem multifunctionality in an intercropped maize-soybean [J]. Acta Agronomica Sinica, 2026, 52(5): 1536-1547.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd