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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (11): 1883-1890.doi: 10.3724/SP.J.1006.2010.01883

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Differential Expression of Genes in Gibberellin-Induced stalk elongation of Sugarcane Analyzed with cDNA-ScoT

WU Jian-Ming1, 2, 3,LI Yang-Rui2, 3, 4 *,WANG Ai-Qin3,YANG Liu2, 3,YANG Li-Tao3   

  1. 1 Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 2 Guangxi Crop Genetic Improvement and Biotechnology Lab, Nanning 530007, China; 3 Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning 530004, China; 4 Sugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China
  • Received:2010-01-04 Revised:2010-06-28 Online:2010-11-12 Published:2010-08-30
  • Contact: LI Yang-Rui,E-mail:liyr@gxaas.net

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Abstract: The present study was conducted to investigate the molecular mechanisms of gibberellin-induced stalk internodes elongation in sugarcane. Using sugarcane variety ROC22, the plant leaves were treated with spraying gibberellic acid (GA3) at 200 mg L–1 at early elongation stage, and spraying water as a control. Samples of young stalks were taken and the differentially expressed genes were analyzed with cDNA-SCoT technique. The results showed that 30 fragments of differentially expressed genes were obtained by screening 700 cDNA fragments with 46 single primers. The expressions of 18 genes were up-regulated, and 12 genes down-regulated by GA3 induction, and the sequences of 16 TDFs showed high similarity to the registered genes in the NCBI database. These genes were divided into six categories by functional analysis, including energy and metabolism-related genes (accounting for 20.0%), unknown functional proteins (accounting for 20.0%), unknown genes (accounting for 46.7%), signal transduction-related genes (accounting for 6.7%), transcription factor-related genes (accounting for 3.3%), and cell apoptosis (accounting for 3.3%). This suggested that the differential expression of genes in the stalks can be analyzed with SCoT technique, and some fragments may be related to the stalk elongation of surgarcane.

Key words: Gibberellin, Sugarcane, cDNA-SCoT, Differential expression

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