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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (11): 1883-1890.doi: 10.3724/SP.J.1006.2010.01883

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Differential Expression of Genes in Gibberellin-Induced stalk elongation of Sugarcane Analyzed with cDNA-ScoT

WU Jian-Ming1, 2, 3,LI Yang-Rui2, 3, 4 *,WANG Ai-Qin3,YANG Liu2, 3,YANG Li-Tao3   

  1. 1 Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 2 Guangxi Crop Genetic Improvement and Biotechnology Lab, Nanning 530007, China; 3 Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning 530004, China; 4 Sugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China
  • Received:2010-01-04 Revised:2010-06-28 Online:2010-11-12 Published:2010-08-30
  • Contact: LI Yang-Rui,E-mail:liyr@gxaas.net

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Abstract: The present study was conducted to investigate the molecular mechanisms of gibberellin-induced stalk internodes elongation in sugarcane. Using sugarcane variety ROC22, the plant leaves were treated with spraying gibberellic acid (GA3) at 200 mg L–1 at early elongation stage, and spraying water as a control. Samples of young stalks were taken and the differentially expressed genes were analyzed with cDNA-SCoT technique. The results showed that 30 fragments of differentially expressed genes were obtained by screening 700 cDNA fragments with 46 single primers. The expressions of 18 genes were up-regulated, and 12 genes down-regulated by GA3 induction, and the sequences of 16 TDFs showed high similarity to the registered genes in the NCBI database. These genes were divided into six categories by functional analysis, including energy and metabolism-related genes (accounting for 20.0%), unknown functional proteins (accounting for 20.0%), unknown genes (accounting for 46.7%), signal transduction-related genes (accounting for 6.7%), transcription factor-related genes (accounting for 3.3%), and cell apoptosis (accounting for 3.3%). This suggested that the differential expression of genes in the stalks can be analyzed with SCoT technique, and some fragments may be related to the stalk elongation of surgarcane.

Key words: Gibberellin, Sugarcane, cDNA-SCoT, Differential expression

[1]Hironori T, Mut Y A, Makoto M. The gibberellin signaling pathway is regulated by the appearance and disappearance of SLENDER RICE1 in nuclei. Plant Cell, 2002, 14: 57–70
[2]Moore P H, Buren L L. Gibberellin studies with sugarcane: I. Cultivar differences in growth responses to gibberellic acid. Crop Sci, 1978, 18: 443–446
[3]Buren L L, Moore P H, Yamasaki Y. Gibberellin studies with sugarcane: II. Hand-sampled field trials. Crop Sci, 1979, 19: 425–428
[4]Most B H, Vlitos A J. Gibberellins of sugarcane. Plant Physioogyl, 1966, 41: 1090–1094
[5]Reid J B, Botwright A N, Smith J J, O’Neill D P, Kerckhoffs L H J. Control of gibberellin levels and gene expression during deetiolation in pea. Plant Physiol, 2002, 123: 734–741
[6]Stavang J A, Lindgard B, Emtsen A, Lid S E, Moe R, Olsen J E. Thermoperiodic stem elongation involves transcriptional regulation of gibberellin deactivation in pea. Plant Physiol, 2005, 138: 2344–2354
[7]Dai M, Zhao Y, Ma Q, Hu Y F, Hedden P, Zhang Q F, Zhou D X. The rice YABBY1 gene is involved in the feedback regulation of gibberellin metabolism. Plant Physioy, 2007, 144: 121–133
[8]Weller J L, Hecht V, Vander Schoor J K, Davidson S E, Ross J J. Light regulation of gibberellin biosynthesis in pea is mediated through the COP1/HY5 pathway. Plant Cell, 2009, 10: 1–14
[9]Collard B C Y, Mackill D J. Start codon targeted (SCoT) polymorphism: A simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Mol Biol Rep, 2009, 27: 86–93
[10]Joshi C, Zhou H, Huang X Q Chiang V L. Context sequences of translation initiation codon in plants. Plant Mol Biol, 1997, 35: 993–1001
[11]Sawant S V, Singh P K, Gupta S K, Madnala R, Tuli R. Conserved nucleotide sequences in highly expressed genes in plants. J Genet, 1999, 78: 123–131
[12]Xiong F-Q(熊发前), Tang R-H(唐荣华), Chen Z-L(陈忠良), Pan L-H(潘玲华), Zhuang W-J(庄伟建). SCOT: a novel gene targeted marker technique based on the translation start codon. Mol Plant Breed (分子植物育种), 2009, 7(3): 635–638 (in Chinese with English abstract)
[13]Giranton J L, Dumas C, Cock J M, Gaude T. The integral membrane S-locus receptor kinase of Brassica has serine/threonine kinase activity in a membranous environment and spontaneously forms oligomers in planta. Proc Natl Acad Sci USA, 2000, 97: 3759–3764
[14]Hajouj T, Michelis R, Gepstein S. Cloning and characterization of a receptor-like protein kinase gene associated with senescence. Plant Physiol, 2000, 124: 1305–1314
[15]Clark S E, Williams R W, Meyerowitz E M. The CLAVATA1 gene encodes a putative receptor kinase that controls shoot and floral meristem size in Arabidopsis. Cell, 1997, 89: 575–585
[16]Sessa G D, Ascenzo M, Loh Y T, Martin G B. Biochemical properties of two protein kinases involved in disease resistance signaling in tomato. J Biol Chem, 1998, 273: 15860–15865
[17]Torii K U. The Arabidopsis ERECTA gene encodes aputative receptor protein kinase with extracellular leucine-rich repeats. Plant Cell, 1996, 8: 735–746
[18]Hong S W, Jon J H, Kwak J M, Nam H G. Identification of a receptor-like protein kinase gene rapidly induced by abscisic acid, dehydration, high salt and cold treatments in Arabidopsis thaliana. Plant Physiol, 1997, 113: 1203–1212
[19]Wu J-M(吴建明), Li Y-R(李杨瑞), Yang L(杨柳), Wang A-Q(王爱勤), Yang L-T(杨丽涛). Relationship between gibberellin-induced internode elongation and endogenous hormone changes in sugarcane. Chin J Trop Crops (热带作物学报), 2009, 30(10): 1452–1456 (in Chinese with English abstract)
[20]Lalty J H, Lee B M, Wright P E. Zinc finger proteins: new insights into structural and functional diversity. Curr Opin Struct Biol, 2001, 11: 39–46
[21]Liu H-M(刘梅芳), Zhou S-F(周淑芬), Xu G-L(徐桂磊), Liu H-Q(刘华清), Wang F(王锋). Expression analysis of a rice CCCH-type zinc finger gene. Fujian J Agric Sci (福建农业学报), 2008, 23: 225–230 (in Chinese with English abstract)
[22]Yue C-W(岳昌武), Xiao J(肖静), Ling X(凌锌), Zeng N(曾霓). Effect of low temperature stress on sweet potato S-adenosyl methionine synthetase gene expression. Agric Sci & Technol (农业科学与技术), 2008, 9(1): 11–14 (in Chinese with English)
[23]Jia L-N(贾丽娜), Zhang H-J(张慧杰), Zhang Y-M(张晏萌), Yang Y-R(杨玉荣), Yu A-L(余爱丽). Cloning and sequence analysis of SAMS gene from Zea mays. J Hebei Agric Sci (河北农业科学), 2008, 12(8): 53–55 (in Chinese with English abstract)
[24]Murase K, Hirano Y, Sun T P, Hakoshima T. Gibberellin-induced DELLA recognition by the gibberellin receptor GID1. Nature, 2008, 456: 459–463
[25]Ueguchi-Tanaka M, Ashikari M, Nakajima M, Itoh H, Katoh E, Kobayashi M, Chow T Y, Hsing Y C, Kitano H, Yamaguchi I, Matsuoka M. GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin. Nature, 2005, 437: 693–698
[26]Ueguchi-Tanaka M, Nakajima M. Katoh E, Ohmiyaa H, Asanoa K, Sajic S, Hongyuc X, Ashikaria M, Kitanoa H, Yamaguchid I, Matsuoka M. Molecular interactions of a soluble gibberellin receptor, GID1, with a rice DELLA protein, SLR1, and gibberellin. Plant Cell, 2007, 19: 2140–2155
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