Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (11): 1891-1901.doi: 10.3724/SP.J.1006.2010.01891

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Up and Down Regulation Genes and Relative Pathway during Cotton Fiber Elongation

LENG Xue,JIA Yin-Hua,DU Xiong-Ming*   

  1. Cotton Research Institute,Chinese Academy of Agricultural Sciences,Anyang 455000,China
  • Received:2010-03-03 Revised:2010-05-23 Online:2010-11-12 Published:2010-08-10
  • Contact: DU Xiong-ming,E-mail:duxm@cricaas.com.cn,Tel:0372-2525352

PDF

1904

Cited

1

Abstract: The fiber development is a crucial factor, influencing cotton yield and quality. The elongation period of fiber development is the key time to determine the fiber’s final length. The mechanism of fiber elongation is not known clearly now. We hope to find some information about it by using wild type and mutant microarray. It is an effective method to studying relevant genes of fiber development by comparing mutants and wild-type gene expression profiles. Cotton fiber formed by single-celled trichomes is the perfect material for studying cell elongation mechanism. Ligon lintless (Li1) is a dominant mutant of upland cotton (Gossypium hirsutum L.) and its fiber is extremely short on mature seed, about 4–6 mm in length, but the wild type (li) is normal for plant and fibers. Recently, we constructed a cDNA microarray used the ovule total RNAs of the mutant and its wild-type. The results showed that many genes expressed up- (or down-) regulated between the mutant and wild type from –1 to +7 DPA (day post anthesis), and two genes (XET, CIPK)were validated by RT-PCR and qRT-PCR analysis. These genes were analyzed via the gene ontology and pathway with Molecule Annotation System (MAS) developed by Capitalbio Co, indicating that these differential expressed genes influenced some metabolism pathways including fatty acid metabolism, glycerolipid metabolism, reductive carboxylate cycle et al. The abnormal expression of these genes in the mutant Li1 maybe result in the change of the fatty acids and fat content, and further influence the fiber development.

Key words: Pathway, Fatty acid, cDNA microarray

[1]Zhu Y-Q(朱勇清), Xu K-X(许可香), Chen X-Y(陈晓亚). The polarity transport of IAA in Ligon lintless mutant is weaken. J Plant Physiol Mol Biol (植物生理与分子生物学报), 2003, 29(1): 15–20 (in Chinese with English abstract)
[2]Liu X-J(刘晓杰), Zhang J(张杰), Jia Y-H(贾银华), Du X-M(杜雄明). Effects of plant hormone on the regulation of ligon lintless mutant in cotton. J Anhui Agric Sci (安徽农业科学), 2008, 36(35): 15460–15469 (in Chinese with English abstract)
[3]Cheng C-H(程超华), Wang X-D(王学德), Yao Y-L(姚艳玲). Inducement of fiber cell elongation from ovule of lintless mutant (Ligon cotton, Gossypium hirsutum L.) in vitro with IAA and GA3. Acta Agron Sin (作物学报), 2005, 31(2): 229–233 (in Chinese with English abstract)
[4]Jiang S-L(蒋淑丽), Wang X-D(王学德). The accumulation of biochemical components in ovule of cotton fiber mutants during the ovule development. J Zhejiang Univ (Agric & Life Sci) (浙江大学学报·农业与生命科学版), 2002, 28(1): 16–21 (in Chinese with English abstract)
[5]Kohel R J, Quisenberry J E, Benedict C R. Incorporation of
[14C] glucose into crystalline cellulose in aberrant fibers of a cotton mutant. Crop Sci, 1993, 33: 1036–1040
[6]Cheng C H, Wang X D, Ni X Y. Observation of fiber ultrastructure of Ligon lintless mutant in upland cotton during fiber elongation. Chin Sci Bull, 2005, 50(2): 126–130
[7]Chen J G, Du X M, Zhou X. Levels of cytokinins in the ovules of cotton mutants with altered fiber development. J Plant Growth Regul, 1997, 16: 181–185
[8]Dixon D C, Seagull R W, TriPlett B A. Changes in the acellroulation of a- and p-tubulinisotypes during cotton fiber development. Plant Physiol, 1994, 105: 1347–1353
[9]Ji S J, Lu Y C, Li J, Wei G, Liang X J, Zhu Y X. A beta-tubulin- like cDNA expressed specifically in elongating cotton fibers induces longitudinal growth of fission yeast. Biochem Biophys Res Commun, 2002, 296: 1245–1250
[10]Ferguson D L, Turley R B, Kloth R H. Identification of a d-TIP cDNA clone and determination of related A and D genome subfamilies in Gossypium species. Plant Mol Biol, 1997, 34: 111–118
[11]Harmer S E, Orford S J, Timmis J N. Characterization of six alpha-expansin genes in Gossypium hirsutum (upland cotton). Mol Gene Genome, 2002, 268: 1–9
[12]Song P, Allen R D. Identification of a cotton fiber-specific acyl carrier protein cDNA by differential display. Biochem Biophys Acta, 1997, 1351: 305–312
[13]Wilkins T A. Vacuolar H+-ATPase 69-kilodalton catalytic subunit cDNA from developing cotton (Gossypium hirsutum) ovules. Plant Physiol, 1993, 102: 679–680
[14]Wan C Y, Wilkins T A. Isolation of multiple cDNAs encoding the vacuolar H+-ATPase subunit B from developing cotton (Gossypium hirsutum L.) ovules. Plant Physiol, 1994, 106: 393–394
[15]Hasenfratz M P, Tsou C L, Wilkins T A. Expression of two related vacuolar H+-ATPase 16-kilodalton proteolipid genes is differentially regulated in a tissue-specific manner. Plant Physiol, 1995, 108: 1395–1404
[16]Andrawis A, Solomon M, Delmer D P. Cotton fiber annexins: a potential role in the regulation of callose synthase. Plant J, 1993, 3: 763–772
[17]Shin H, Brown Jr R M. GTPase activity and biochemical characterization of a recombinant cotton fiber annexin. Plant Physiol, 1999, 119: 925–934
[18]Kawai M, Aotsuka S, Uchimiya H. Isolation of a cotton CAP gene: a homologue of adenylyl cyclase-associated protein highly expressed during fiber elongation. Plant Cell Physiol, 1998, 39: 1380–1383
[19]Wang S, Zhao G H, Jia Y H, Du X M. Molecular cloning, and characterization of an adenylyl cyclase-associated protein from Gossypium arboreum L. Agric Sci China, 2009, 8(7): 777–783
[20]Shi Y H, Zhu S W, Mao X Z, Feng J X, Qin Y M, Zhang L, Cheng J, Wei L P, Wang Z Y, Zhu Y X. Transcriptome profiling, molecular biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation. Plant Cell, 2006, 18: 651–664
[21]Qin Y M , Hu C Y, Pang Y, Kastaniotis A J, Hiltunen J K, Zhu Y X. Saturated very-long-chain fatty acids promote cotton fiber and Arabidopsis cell elongation by activating ethylene biosynthesis. Plant Cell, 2007, 19: 3692–3704
[22]Bolton J J, Soliman K M, Wilkins T A, Jenkins J N. Aberrant expression of critical genes during secondary cell wall biogenesis in a cotton mutant, Ligon lintless-1 (Li-1). Comparative Function Genom, DOI: 10.1155/2009/659301
[23]Yang Y H, Dudoit S, Luu P, Lin D M, Peng V, Ngai J, Speed T P. Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation. Nucl Acids Res, 2002, 30: e15
[24]Tusher V, Tibshirani R, Chu G. Significance analysis of microarrays applied to transcriptional responses to ionizingradiation. Proc Natl Acad Sci USA, 2001, 98: 5116–5121
[25]Murry E, Soonpaa M H, Reinecke H. Haematopoietic stem cells do not transdifferentiate into cardiac myocytes in myocardial infarcts. Nature, 2004, 428: 664-668
[26]Page R A, Okada S, Harwood J L. Acetyl-CoA carboxylase exerts strong flux control over lipid synthesis in plants. Biochim Biophys Acta, 1994, 1210: 369–372
[27]Post-Beittenmiller D, Roughan P G, Ohlrogge J B. Regulation of plant fatty acid biosynthesis. Analysis of acyl-Coenzyme A and acyl-acyl carrier protein substrate pools in spinach and pea chloroplasts. Plant Physiol, 1992, 100: 923–930
[28]Kanehisa M, Goto S. KEGG: Kyoto encyclopedia of genes and genomes. Nucl Acids Res, 2000, 28, 27–30
[29]De Fabiani E, Mitro N, Godio C, Gilardi F, Caruso D, Crestani M. Bile acid signaling to the nucleus: finding new connections in the transcriptional regulation of metabolic pathways. Biochimie, 2004, 86: 771–778
[30]Wanjie S W, Welti R, Moreau R A, Chapman K D. Identification and quantification of glycerolipidsin cotton fibers: reconciliation with metabolicpathway predictions from DNA databases. Lipids, 2005, 40: 8
[31]Qin Y M, Pujol F A, Shi Y H, Feng J X, Liu Y M, Kastaniotis A J, Hiltunen J K, Zhu Y X. Cloning and functional characterization of two cDNAs encoding NADPH-dependent 3-ketoacyl-CoA reductases from developing cotton fibers. Cell Res, 2005, 15: 465–473
[32]Gou J Y, Wang L J, Chen S P, Hu W L, Chen X Y. Gene expression and metabolite profiles of cotton fiber during cell elongation and secondary cell wall synthesis. Cell Res, 2007, 17: 422–434
[1] XU Jing, GAO Jing-Yang, LI Cheng-Cheng, SONG Yun-Xia, DONG Chao-Pei, WANG Zhao, LI Yun-Meng, LUAN Yi-Fan, CHEN Jia-Fa, ZHOU Zi-Jian, WU Jian-Yu. Overexpression of ZmCIPKHT enhances heat tolerance in plant [J]. Acta Agronomica Sinica, 2022, 48(4): 851-859.
[2] ZHANG Yan-Bo, WANG Yuan, FENG Gan-Yu, DUAN Hui-Rong, LIU Hai-Ying. QTLs analysis of oil and three main fatty acid contents in cottonseeds [J]. Acta Agronomica Sinica, 2022, 48(2): 380-395.
[3] SHI Lei, MIAO Li-Juan, HUANG Bing-Yan, GAO Wei, ZHANG Zong-Xin, QI Fei-Yan, LIU Juan, DONG Wen-Zhao, ZHANG Xin-You. Characterization of the promoter and 5'-UTR intron in AhFAD2-1 genes from peanut and their responses to cold stress [J]. Acta Agronomica Sinica, 2021, 47(9): 1703-1711.
[4] XUE Xiao-Meng, WU JIE, WANG Xin, BAI Dong-Mei, HU Mei-Ling, YAN Li-Ying, CHEN Yu-Ning, KANG Yan-Ping, WANG Zhi-Hui, HUAI Dong-Xin, LEI Yong, LIAO Bo-Shou. Effects of cold stress on germination in peanut cultivars with normal and high content of oleic acid [J]. Acta Agronomica Sinica, 2021, 47(9): 1768-1778.
[5] QIU Hong-Mei, CHEN Liang, HOU Yun-Long, WANG Xin-Feng, CHEN Jian, MA Xiao-Ping, CUI Zheng-Guo, ZHANG Ling, HU Jin-Hai, WANG Yue-Qiang, QIU Li-Juan. Research progress on genetic regulatory mechanism of seed color in soybean (Glycine max) [J]. Acta Agronomica Sinica, 2021, 47(12): 2299-2313.
[6] CHEN Tong-Rui, LUO Yan-Jun, ZHAO Pan-Ting, JIA Hai-Yan, MA Zheng-Qiang. Overexpression of TaJRL53 enhances the Fusarium head blight resistance in wheat [J]. Acta Agronomica Sinica, 2021, 47(1): 19-29.
[7] LU Geng,TANG Xin,LU Jun-Xing,LI Dan,HU Qiu-Yun,HU Tian,ZHANG Tao. Cloning and function analysis of a type 2 diacylglycerol acyltransferase (DGAT2) from Perilla frutescens [J]. Acta Agronomica Sinica, 2020, 46(8): 1283-1290.
[8] Jian-Bin GUO,Li HUANG,Nian LIU,Huai-Yong LUO,Xiao-Jing ZHOU,Wei-Gang CHEN,Bei WU,Dong-Xin HUAI,Xiao-Ping REN,Hui-Fang JIANG. Novel peanut genotype with low behenic acid developed from recombinant inbred lines [J]. Acta Agronomica Sinica, 2020, 46(5): 661-667.
[9] SUN Qi, ZHAO Zhi-Chao, ZHANG Jin-Hui, ZHANG Feng, CHENG Zhi-Jun, ZOU De-Tang. Genetic analysis and fine mapping of a sheathed panicle mutant sui2 in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2020, 46(11): 1734-1742.
[10] XIANG Li-Yuan,XU Kai,SU Jing,WU Chao,YUAN Xiong,ZHENG Xing-Fei,DIAO Ying,HU Zhong-Li,LI Lan-Zhi. Genetic dissection of combining ability and heterosis of rice agronomic traits based on pathway analysis [J]. Acta Agronomica Sinica, 2019, 45(9): 1319-1326.
[11] WANG Yan-Hua,XIE Ling,YANG Bo,CAO Yan-Ru,LI Jia-Na. Flowering genes in oilseed rape: identification, characterization, evolutionary and expression analysis [J]. Acta Agronomica Sinica, 2019, 45(8): 1137-1145.
[12] CHEN Ying,ZHANG Sheng-Rui,WANG Lan,WANG Lian-Zheng,LI Bin,SUN Jun-Ming. Characteristics of oil components and its relationship with domestication of oil components in wild and cultivated soybean accessions [J]. Acta Agronomica Sinica, 2019, 45(7): 1038-1049.
[13] Zhi-Hong HOU,Yan WU,Qun CHENG,Li-Dong DONG,Si-Jia LU,Hai-Yang NAN,Zhuo-Ran GAN,Bao-Hui LIU. Creation of high oleic acid soybean mutation plants by CRISPR/Cas9 [J]. Acta Agronomica Sinica, 2019, 45(6): 839-847.
[14] Jian-Bin GUO,Bei WU,Wei-Gang CHEN,Li HUANG,Yu-Ning CHEN,Xiao-Jing ZHOU,Huai-Yong LUO,Nian LIU,Xiao-Ping REN,Hui-Fang JIANG. Stability of major fatty acids contents of peanut varieties grown in different ecological regions [J]. Acta Agronomica Sinica, 2019, 45(5): 676-682.
[15] HUANG Bing-Yan,QI Fei-Yan,SUN Zi-Qi,MIAO Li-Juan,FANG Yuan-Jin,ZHENG Zheng,SHI Lei,ZHANG Zhong-Xin,LIU Hua,DONG Wen-Zhao,TANG Feng-Shou,ZHANG Xin-You. Improvement of oleic acid content in peanut (Arachis hypogaea L.) by marker assisted successive backcross and agronomic evaluation of derived lines [J]. Acta Agronomica Sinica, 2019, 45(4): 546-555.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd