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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (11): 1891-1901.doi: 10.3724/SP.J.1006.2010.01891

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Up and Down Regulation Genes and Relative Pathway during Cotton Fiber Elongation

LENG Xue,JIA Yin-Hua,DU Xiong-Ming*   

  1. Cotton Research Institute,Chinese Academy of Agricultural Sciences,Anyang 455000,China
  • Received:2010-03-03 Revised:2010-05-23 Online:2010-11-12 Published:2010-08-10
  • Contact: DU Xiong-ming,E-mail:duxm@cricaas.com.cn,Tel:0372-2525352

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Abstract: The fiber development is a crucial factor, influencing cotton yield and quality. The elongation period of fiber development is the key time to determine the fiber’s final length. The mechanism of fiber elongation is not known clearly now. We hope to find some information about it by using wild type and mutant microarray. It is an effective method to studying relevant genes of fiber development by comparing mutants and wild-type gene expression profiles. Cotton fiber formed by single-celled trichomes is the perfect material for studying cell elongation mechanism. Ligon lintless (Li1) is a dominant mutant of upland cotton (Gossypium hirsutum L.) and its fiber is extremely short on mature seed, about 4–6 mm in length, but the wild type (li) is normal for plant and fibers. Recently, we constructed a cDNA microarray used the ovule total RNAs of the mutant and its wild-type. The results showed that many genes expressed up- (or down-) regulated between the mutant and wild type from –1 to +7 DPA (day post anthesis), and two genes (XET, CIPK)were validated by RT-PCR and qRT-PCR analysis. These genes were analyzed via the gene ontology and pathway with Molecule Annotation System (MAS) developed by Capitalbio Co, indicating that these differential expressed genes influenced some metabolism pathways including fatty acid metabolism, glycerolipid metabolism, reductive carboxylate cycle et al. The abnormal expression of these genes in the mutant Li1 maybe result in the change of the fatty acids and fat content, and further influence the fiber development.

Key words: Pathway, Fatty acid, cDNA microarray

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